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Development and preliminary application of gE enzyme-linked immunosorbent assay for detection of the antibody to gE protein of Pseudorabies virus in pigs

Published online by Cambridge University Press:  13 June 2008

Tang Yong ,
Chen Huan-Chun ,
Qin Ya-Li ,
He Qi-Gai ,
Jin Mei-Lli ,
Wu Bin  and
Liu Zheng-Fei
Tang Yong
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China Department of Bioengineering, JiNan University, Guangzhou 51063, China
Chen Huan-Chun*
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Qin Ya-Li
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
He Qi-Gai
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Jin Mei-Lli
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Wu Bin
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Liu Zheng-Fei
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. Email: Hzauvet@public.wh.hb.cn
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Abstract

To differentiate pigs infected with Pseudorabies virus (PrV) from pigs vaccinated with gE-PrV, a glycoprotein E enzyme-linked immunosorbent assay (gE-ELISA) based on recombinant glycoprotein E (gE) (which was expressed by Escherichia coli, purified, denatured and renatured) was developed. By testing 115 serum samples, the diagnostic specificity and sensitivity of the developed gE-ELISA were evaluated to be 94.5% and 96.7%, respectively. Five serum samples were tested with plates from five lots, and the results had a coefficient of variation of less than 10%, showing good reproducibility of gE-ELISA. This gE-ELISA was compared with a commercial blocking ELISA by testing 356 serum samples. The agreement rate of the two assays was 92.13% (328/356). These results suggested that the gE-ELISA developed in our laboratory could be used in differentiating PrV-infected and gE-PrV-vaccinated pigs.

Keywords

Pseudorabies virusglycoprotein Eenzyme-linked immunosorbent assay (ELISA)
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 79 - 83
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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