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The effects of diet protein levels on the growth, body composition and digestive enzyme activities of the Barbodes caldwelli juvenile

Published online by Cambridge University Press:  29 January 2010

Lv Yao-Ping ,
Chen Jian-Ming ,
Ye Jin-Yun ,
Huang Xu-Xiong ,
Lao Shen-Ying ,
Shen Bin-Qian ,
Yao Zi-Liang ,
Guo Jian-Lin  and
Ye Li-Ping
Lv Yao-Ping
Affiliation:
College of Chemistry and Life Sciences, Lishui University, Lishui 323000, China College of Animal Science, Zhejiang University, Hangzhou 310029, China
Chen Jian-Ming
Affiliation:
Institute of Freshwater Fisheries of Zhejiang Province, Huzhou 313001, China
Ye Jin-Yun
Affiliation:
Institute of Freshwater Fisheries of Zhejiang Province, Huzhou 313001, China
Huang Xu-Xiong
Affiliation:
College of Aqua-life Science & Technology, Shanghai Fisheries University, Shanghai 200090, China
Lao Shen-Ying
Affiliation:
College of Chemistry and Life Sciences, Lishui University, Lishui 323000, China
Shen Bin-Qian
Affiliation:
Institute of Freshwater Fisheries of Zhejiang Province, Huzhou 313001, China
Yao Zi-Liang
Affiliation:
Lishui Fishery Technology Promotion Station of Zhejiang Province, Lishui 323000, China
Guo Jian-Lin
Affiliation:
Institute of Freshwater Fisheries of Zhejiang Province, Huzhou 313001, China
Ye Li-Ping
Affiliation:
College of Chemistry and Life Sciences, Lishui University, Lishui 323000, China
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Abstract

Seven isoenergetic semi-purified test diets containing graded levels of protein ranging from 20 to 50% were formulated using fish meal and casein as the protein sources. Test diets were fed to triplicate groups of Barbodes caldwelli juveniles with initial body weights of 1.26±0.02 g for eight weeks. The results indicated no significant effect of dietary protein levels on survival rate, relative weight of the viscera and relative weight of the liver in the juvenile fish. The weight gain and specific growth rate of the fish were found to be greater as dietary protein levels increased from 20 to 35%, but were not affected significantly as dietary protein level increased from 35 to 50%. Feed efficiencies did not differ significantly when fish were fed diets with protein levels from 30 to 50%, but were significantly higher than those of fish fed diets with protein levels of 20 and 25%. The protein efficiency ratio (PER) was negatively correlated with diet protein level (x) (PER=3.006−0.03251x, R=0.9366). There was no significant effect of dietary protein levels on carcass moisture, crude protein and ash. However, carcass lipid levels (L) decreased with an increase in dietary protein level (x) (L=8.2169−0.0458x, R=0.8551). There was no significant variation in hepatopancreas protease activity among the tests. Intestine protease activity and hepatopancreas amylase activity were increased to some extent, and then decreased as dietary protein levels continued to rise. The intestine amylase activity (IAA) of the juveniles was negatively correlated with dietary protein level (x) (IAA=84.625−0.9147x, R=0.8463). It was estimated that the suitable protein level for the B. caldwelli juvenile is 34% (the broken-line model was used to regress the relationship of the weight gain of the juvenile and dietary protein level).

Keywords

Barbodes caldwelli juveniledietary protein requirementweight gainspecific growth ratefeed efficiencyprotein efficiency ratiodigestive enzyme activities
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 199 - 205
Copyright
Copyright © China Agricultural University 2009

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References

AOAC (1995) Official Methods of Analysis of AOAC International, 16th edn, Cunniff, P (ed.). Arlington, VA: AOAC International.Google Scholar
Bernfeld, P (1955) Amylases, α and β. Methods in Enzymology 1: 149–158.Google Scholar
Bradford, MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Analytical Biochemistry 72: 248–254.Google Scholar
Cho, CY and Kaushik, SJ (1985) Effect of protein intake on metabolizable and energy values of fish diets. In: Cowey, CB, Mackie, AM and Bell, JB (editors). Nutrition Feeding in Fish. London: Academic Press. pp. 95–117.Google Scholar
Das, KN and Tripathi, SD (1991) Studies on digestive enzymes of grass carp, Ctenopharyngodon idella. Aquaculture 92: 2–32.Google Scholar
Elangovan, A and Shim, KF (1997) Growth response of juvenile Barbodes altus fed isocaloric diets with variable protein levels. Aquaculture 158: 321–329.CrossRefGoogle Scholar
Giri, SS, Sahoo, SK and Sahu, AK (2003) Effect of dietary protein level on growth, food feed utilization and body composition of hybrid Clarias catfish (Clarias batrachus×Clarias gariepinus). Animal Feed Science and Technology 104: 169–178.CrossRefGoogle Scholar
Gunasekera, RM, De Silva, SS, Collins, RA, et al. (2000) Effect of dietary protein level on growth and food utilization in juvenile Murray cod Maccullochella peelii peelii. Aquaculture Research 31: 181–187.Google Scholar
Jana, SN, Garg, SK, Barman, UK, et al. (2006) Effect of varying protein levels on growth and production of Chanos chanos in inland saline ground water: laboratory and field studies. Aquaculture International 14: 479–498.CrossRefGoogle Scholar
Kim, K, Kayes, TB and Amundson, CH (1991) Purified diet development and re-evaluation of the dietary protein requirements of Artic charr, Salvelinus alpinus. Journal of Fish Biology 22: 705–712.Google Scholar
Lin, D, Mao, YQ and Cai, FS (1980) Experiments on the protein requirements of grass carp (Ctenopharyngodon idella). Acta Hydrobiologica Sinica 7: 207–212 (in Chinese with English abstract).Google Scholar
Lochmann, RT and Phillips, H (1994) Dietary protein requirement of juvenile golden shiners (Notemigonus crysoleucas) and goldfish (Carassius auratus) in aquaria. Aquaculture 128: 277–285.Google Scholar
Lv, YP, Yao, ZL and Lao, SY (2007) The biological features and cage-culture technique of Barbodes caldwelli. Journal of Lishui University 29(4): 36–39 (in Chinese with English abstract).Google Scholar
Lv, YP, Huang, XX and Yang, YB (2008) Analysis and evaluation of the nutritive composition and quality in the muscle of Spinibarbus caldwelli Nichols. Journal of Huazhong Agricultural University 27(1): 86–90 (in Chinese with English abstract).Google Scholar
Mohanty, SS and Samantaray, K (1996) Effects of varying levels of dietary protein on the growth performance and feed conversion efficiency of snake head, Channa striata, fry. Aquaculture Nutrition 2: 89–94.Google Scholar
National Research Council (NRC). (1993) Nutrient Requirements of Fishes. Washington, DC: National Academy of Sciences.Google Scholar
Siddiqui, AQ, Howlader, MS and Adam, AA (1988) Effects of dietary protein levels on growth, feed conversion and protein utilization in fry and young Nile tilapia, Oreochromis nilotica. Aquaculture 70: 63–73.CrossRefGoogle Scholar
Satpathy, BB, Mukherjee, D and Ray, AK (2003) Effect of dietary protein and lipid levels on growth, feed conversion and body composition in rohu, Labeo rohita (Hamilton), fingerlings. Aquaculture Nutrition 9: 17–24.Google Scholar
Shiau, SY and Huang, SL (1989) Optimal dietary protein level of hybrid tilapia Oreochromis nilotica×Oreochromis aureus) reared in seawater. Aquaculture 81: 119–127.CrossRefGoogle Scholar
Shyong, WJ, Huang, CH and Chen, HC (1998) Effect of dietary protein concentration on growth and muscle composition of juvenile Zacco barbata. Aquaculture 167: 35–42.Google Scholar
Yang, SD, Lin, TS and Liou, CH (2003) Influence of dietary protein levels on growth performance, carcass composition and liver lipid classes of juvenile Spinibarbus hollandi. Aquaculture Research 34: 661–666.CrossRefGoogle Scholar