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Effects of dietary energy levels on ovarian and uterine expression of IGF-1R and EGFR mRNA in prepubertal gilts

Published online by Cambridge University Press:  01 October 2008

Li Bo ,
Zhang He ,
Zhang Jing ,
Sun Bo-Xing ,
Chen Lu ,
Sun Yan-Ling  and
Zhou Xu
Li Bo
Affiliation:
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China National Feed Engineering Technology Research Center, Beijing 100094, China
Zhang He
Affiliation:
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
Zhang Jing
Affiliation:
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
Sun Bo-Xing
Affiliation:
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
Chen Lu
Affiliation:
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
Sun Yan-Ling
Affiliation:
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
Zhou Xu*
Affiliation:
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
*
*Corresponding author. E-mail: zhouxu@jluhp.edu.cn
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Abstract

Nine prepubertal gilts (JunMu No. 1) were randomly allocated into three groups (n=3) and fed with a high-energy diet (Group H), a low-energy diet (Group L), or a moderate-energy diet (Group M) for 14 days. Free access to water was provided throughout the research period. Ovaries and uteri were collected after the energy treatments, and processed for determination of the absolute quantities of insulin-like growth factor receptor (IGF-1R) and epidermal growth factor receptor (EGFR) mRNA, using real-time polymerase chain reaction (PCR). The expression of IGF-1R and EGFR mRNA in ovaries and uteri was significantly ranked as: Group H>Group M>Group L (P<0.05). This result suggests that high energy intake markedly enhanced the ovarian and uterine expression of IGF-1R and EGFR in prepubertal gilts, whereas insufficient energy intake markedly inhibited such expression. IGF-1R and EGFR may be involved in mediating the effects of energy intake on the development of the reproductive system in prepubertal gilts.

Keywords

dietary energy levelinsulin-like growth factor receptor (IGF-1R)epidermal growth factor receptor (EGFR)prepubertal gilts
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 159 - 164
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 225–229

References

Floves, JM, Sanchez, MA and Garcia, P (1998) Immunohistochemical localization of epidermal growth factor, transforming growth factor-α and growth factor-βs in the caprine peri-implantation period. Theriogenology 50: 931–944.Google Scholar
Fowden, AL (2003) The insulin-like growth factors and feto-placental growth. Placenta 24(8–9): 803–812.CrossRefGoogle ScholarPubMed
Heid, CA, Stevens, J, Livak, KJ and Williams, PM (1996) Real time quantitative PCR. Genome Research 6(10): 986–994.CrossRefGoogle ScholarPubMed
Martin, MA, Serradas, P, Ramos, S, et al. (2005) Protein-caloric food restriction affects insulin-like growth factor system in fetal Wistar rat. Endocrinology 146(3): 1364–1371.CrossRefGoogle ScholarPubMed
Muaku, SM, Beauloye, V, Thissen, JP, Underwood, LE, Ketesleqers, JM and Maiter, D (1995) Effects of maternal protein malnutrition on fetal growth, plasma insulin-like growth factors, insulin-like growth factor binding protein and liver insulin-like growth factor gene expression in the rat. Pediatric Research 37: 334–342.CrossRefGoogle ScholarPubMed
Reynolds, TS, Wathes, DC, Aitken, RP and Wallace, JM (1997) Effect of maternal nutrition on components of the insulin like growth factor (IGF) system and placental growth. Journal of Endocrinology 152(Suppl.): 248–249.Google Scholar
Shen, ZM, Seyfert, HM, Löhrke, B, et al. (2004) An energy-rich diet causes rumen papillae proliferation associated with more IGF type 1 receptors and increased plasma IGF-1 concentrations in young goats. Biochemical and Molecular Actions of Nutrients 134: 11–17.Google ScholarPubMed
Souaze, F, Ntodou-Thome, A, Tran, CY, Rostene, W and Forgez, P (1996) Quantitative RT-PCR: limits and accuracy. Biotechniques 21: 280–285.CrossRefGoogle ScholarPubMed
Trentacoste, SV, Friedmann, AS, Youker, RT, Breckenridge, CB and Zirkin, BR (2001) Atrazine effects on testosterone levels and androgen-dependent reproductive organs in peripubertal male rats. Journal of Andrology 22(1): 142–148.CrossRefGoogle ScholarPubMed
Wathes, DC, Dronfield, DM, Perks, CM, Murray, JF and Scaramuzzi, RJ (1996) Nutritional effect on insulin-like growth factor type 1 receptor mRNA in ovine follicles. Journal of Reproduction and Fertility Abstract Series 17: 14–15.Google Scholar