Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-11T09:36:23.320Z Has data issue: false hasContentIssue false
  • English
  • Français

Establishment of regeneration system and transformation of Zm401 gene in Lilium longiflorum×L. formosanum

Published online by Cambridge University Press:  01 October 2008

Li Qiu-Hua ,
Hong Bo ,
Tong Zheng ,
Ma Chao ,
Guan Ai-Nong ,
Yu Jing-Juan  and
Gao Jun-Ping
Li Qiu-Hua
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Hong Bo
Affiliation:
College of Landscape Architecture, Northeast Forestry University, Haerbing 150040, China
Tong Zheng
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Ma Chao
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Guan Ai-Nong
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Yu Jing-Juan
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Gao Jun-Ping*
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: gaojp@cau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

In vitro bulb scales of Lilium longiflorum×L. formosanum were used as explants to develop a highly efficient regeneration system. A high regeneration rate (100%) was reached through organogenesis on basal Murashige and Skoog (MS) medium supplemented with 1.0 mg/l 6-benzylaminopurine (6-BA) and 1.0 mg/l naphthaleneacetic acid (NAA). A genetic transformation system for the lily was developed using an Agrobacterium tumefaciens-mediated method. An improved genetic transformation rate (12‰) was obtained when the explants were pre-cultured for 3 days, immersed in bacterial suspension (OD600≈0.8) for 5 min, and co-cultivated for 5 days. The binary vector pBI121 containing Zm401, a maize pollen-specific gene, was introduced into the Agrobacterium strain LBA4404 and transformed into the explants using the genetic transformation system. Gene integration into the lily genome was confirmed by polymerase chain reaction (PCR) and PCR–Southern analysis. These results could lead to the production of new pollenless lily plants.

Keywords

Lilium longiflorum×L. formosanumZm401regeneration systemtransformation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 113 - 119
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 96–102

References

Bytebier, B, Deboeck, F, Greve, H, de Montagu, M and van Hernalsteens, JP (1987) T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. Proceedings of National Academy of Sciences of the USA 84(15): 5345–5349.CrossRefGoogle ScholarPubMed
Chan, MT, Chang, HH, Ho, SL, Tong, WF and Yu, SM (1993) Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/β-glucuronidase gene. Plant Molecular Biology 22: 491–506.CrossRefGoogle ScholarPubMed
Cheng, M, Fry, JE, Pang, S, et al. (1997) Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiology 115: 971–980.CrossRefGoogle ScholarPubMed
Cohen, A and Meredith, CP (1992) Agrobacterium-mediated transformation of Lilium. Acta Horticulturae 325: 611–618.CrossRefGoogle Scholar
Dai, XY, Yu, JJ, Zhao, Q, Zhu, DY and Ao, GM (2004) Non-coding RNA for ZM401, a pollen-specific gene of maize. Acta Botanica Sinica 46: 497–504.Google Scholar
Delbreil, B, Guerche, P and Jullien, M (1993) Agrobacterium-mediated transformation of Asparagus officinalis L. long-term embryogenic callus and regeneration of transgenic plants. Plant Cell Reports 12(3): 129–132.CrossRefGoogle ScholarPubMed
Grassotti, A and Mercuri, A (1996) Lilium elegans: selection of pollenless clones. Acta Horticulturae 414: 125–128.CrossRefGoogle Scholar
Grimsley, N, Hohn, T, Davies, JW and Hohn, B (1987) Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325: 177–179.CrossRefGoogle Scholar
Hiei, Y, Ohta, S, Komari, T and Kumashiro, T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal 6: 271–282.CrossRefGoogle ScholarPubMed
Hong, B, Zhang, ChQ, Li, QH, Kasuga, M, Yamaguchi-Shinozaki, K and Gao, JP (2005) Agrobacterium-mediated transformation of groundcover chrysanthemum (Dendranthema grandiflorum cv. White Snow) with transcription factor DREB1A gene. Journal of Agricultural Biotechnology 13(3): 304–309.Google Scholar
Hong, B, Tong, Z, Ma, N, et al. (2006a) Heterologous expression of the AtDREB1A gene in chrysanthemum increases drought and salt stress tolerance. Science in China 49(5): 436–445.CrossRefGoogle ScholarPubMed
Hong, B, Tong, Z, Ma, N, Kasuga, M, Yamaguchi-Shinozaki, K and Gao, JP (2006b) Expression of DREB1A gene in transgenic chrysanthemum enhances tolerance to low temperature. Journal of Horticultural Science and Biotechnology 81(6): 1002–1008.CrossRefGoogle Scholar
Hong, B, Tong, Z, Li, QH, et al. (2006c) Regeneration and transformation through somatic embryogenesis, and determination of cold stress tolerance in ground cover chrysanthemum cv. Fall Color. Scientia Agricultura Sinica 39(7): 1443–1450.Google Scholar
Hoshi, Y, Kondo, M, Mori, S, Adachi, Y, Nakano, M and Kobayashi, H (2004) Production of transgenic lily plants by Agrobacterium-mediated transformation. Plant Cell Reports 22(6): 359–364.CrossRefGoogle ScholarPubMed
Hoshi, Y, Kondo, M and Kobayashi, H (2005) Agrobacterium-mediated transformation of Lilium longiflorum. Acta Horticulturae 673(2): 543–547.CrossRefGoogle Scholar
Langeveld, SA, Gerrits, MM, Derks, AFLM, Boonekamp, PM and Bol, JF (1995) Transformation of lily by Agrobacterium. Euphytica 85: 97–100.CrossRefGoogle Scholar
Li, ChX, Liu, JQ, Yu, JJ, Zhao, Q and Ao, GM (2001) Cloning and expression analysis of pollen-specific cDNA ZM401 from Zea mays. Journal of Agricultural Biotechnology 9(4): 374–377.Google Scholar
Ma, JX, Zhao, Q, Yu, JJ and Ao, GM (2005) Ectopic expression of a maize pollen specific gene, ZM401, results in aberrant anther development in tobacco. Euphytica 144: 133–140.CrossRefGoogle Scholar
Mariani, C, Beuckeleer, M, de Truettner, J, Leemans, J and Goldberg, RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347(6295): 737–741.CrossRefGoogle Scholar
Mitsuda, N, Seki, M, Shinozaki, K and OhmeTakagi, M (2005) The NAC transcription factors NST1 and NST2 regulate secondary wall thickening and are required for anther dehiscence. Plant Cell Reports 17(11): 2993–3006.CrossRefGoogle ScholarPubMed
Watad, AA, Yun, DJ, Matsumoto, T, et al. (1998) Microprojectile bombardment-mediated transformation of Lilium longiflorum. Plant Cell Reports 17: 262–267.CrossRefGoogle ScholarPubMed
Yamagishi, M (2003) A genetic model for a pollenless trait in Asiatic hybrid lily and its utilization for breeding. Scientia Horticulturae 98: 293–297.CrossRefGoogle Scholar