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Establishment of regeneration system and transformation of Zm401 gene in Lilium longiflorum×L. formosanum

Published online by Cambridge University Press:  01 October 2008

Li Qiu-Hua ,
Hong Bo ,
Tong Zheng ,
Ma Chao ,
Guan Ai-Nong ,
Yu Jing-Juan  and
Gao Jun-Ping
Li Qiu-Hua
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Hong Bo
Affiliation:
College of Landscape Architecture, Northeast Forestry University, Haerbing 150040, China
Tong Zheng
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Ma Chao
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Guan Ai-Nong
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
Yu Jing-Juan
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Gao Jun-Ping*
Affiliation:
Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: gaojp@cau.edu.cn
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Abstract

In vitro bulb scales of Lilium longiflorum×L. formosanum were used as explants to develop a highly efficient regeneration system. A high regeneration rate (100%) was reached through organogenesis on basal Murashige and Skoog (MS) medium supplemented with 1.0 mg/l 6-benzylaminopurine (6-BA) and 1.0 mg/l naphthaleneacetic acid (NAA). A genetic transformation system for the lily was developed using an Agrobacterium tumefaciens-mediated method. An improved genetic transformation rate (12‰) was obtained when the explants were pre-cultured for 3 days, immersed in bacterial suspension (OD600≈0.8) for 5 min, and co-cultivated for 5 days. The binary vector pBI121 containing Zm401, a maize pollen-specific gene, was introduced into the Agrobacterium strain LBA4404 and transformed into the explants using the genetic transformation system. Gene integration into the lily genome was confirmed by polymerase chain reaction (PCR) and PCR–Southern analysis. These results could lead to the production of new pollenless lily plants.

Keywords

Lilium longiflorum×L. formosanumZm401regeneration systemtransformation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 113 - 119
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 96–102

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