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Expression optimization of subunit fasG of 987P fimbriae in enterotoxigenic Escherichia coli (ETEC)

Published online by Cambridge University Press:  24 April 2009

Luo He-Feng ,
Wang Jie ,
Gou Zhong-Yong  and
Peng Jian
Luo He-Feng
Affiliation:
Department of Animal Nutrition and Feed Science, Huazhong Agricultural University, Wuhan 430070, China
Wang Jie
Affiliation:
Department of Animal Nutrition and Feed Science, Huazhong Agricultural University, Wuhan 430070, China
Gou Zhong-Yong
Affiliation:
Department of Animal Nutrition and Feed Science, Huazhong Agricultural University, Wuhan 430070, China
Peng Jian*
Affiliation:
Department of Animal Nutrition and Feed Science, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. E-mail: pengjian@mail.hzau.edu.cn
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Abstract

Four bioengineered bacteria having the fasG gene were constructed using two Escherichia coli expression strains (BL21 (DE3) and Rossetta (DE3)) with different tRNA profiles and two E. coli expression vectors (pET28a(+) and pGEX-KG) to examine the influence of rare codons on the expression of the fasG gene. The induction expression result of bioengineered bacteria showed that the expression levels of target proteins in Rossetta (DE3), which contained the tRNAs of the rare codons, were higher than in BL21 (DE3), either using pET-fasG or pKG-fasG.

Keywords

cloningenterotoxigenic E. coli (ETEC)fasG987P fimbriaeoptimizing expression
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 1 , April 2009 , pp. 19 - 25
Copyright
Copyright © China Agricultural University 2009

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