Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-28T20:05:59.040Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of isozyme in Pteria penguin R. and offspring from inbreeding and hybridized stocks of Pinctada martensii D.

Published online by Cambridge University Press:  12 February 2007

Wang Mei-Fang ,
Yu Xiang-Yong ,
Liu Yong ,
Mao Yong  and
Liang Fei-Long
Wang Mei-Fang
Affiliation:
Pearl Research Institute of Guangdong Ocean University, Zhanjiang 524025, China
Yu Xiang-Yong*
Affiliation:
Pearl Research Institute of Guangdong Ocean University, Zhanjiang 524025, China
Liu Yong
Affiliation:
Pearl Research Institute of Guangdong Ocean University, Zhanjiang 524025, China
Mao Yong
Affiliation:
Pearl Research Institute of Guangdong Ocean University, Zhanjiang 524025, China
Liang Fei-Long
Affiliation:
Pearl Research Institute of Guangdong Ocean University, Zhanjiang 524025, China
*
*Corresponding author. Email: yuxyong@tom.com
Get access Rights & Permissions [Opens in a new window]

Abstract

The genetic variation of isozymes [esterase (EST), superoxide dismutase (SOD), malate dehydrogenase (MDH), malic enzyme (ME) and glucose-6-phosphate dehydrogenase (G6PD)] extracted from two different adult tissues (adductor muscle and gill) of Pteria penguin R. and offspring from two stocks of Pinctada martensii D. were analysed by vertical polyacrylamide gel electrophoresis. The result showed an obvious and stable variation in the isozyme phenotypes in two different pearl oyster species. The SOD and EST isozymes from gill and MDH, ME and G6PDH from adductor muscle were species-specific. The electrophoretograms of these isozymes could be used as markers to differentiate the two pearl oysters. Between inbreeding and hybridized stocks of P. martensii, the electrophoresis patterns of isozymes were alike, but the similarities of electrophoretograms in the inbreeding progenies were higher than in the hybridized ones. The characteristic electrophoretograms of isozymes could be applied as molecular markers in the breeding of pearl oysters.

Keywords

Pinctada martensiiPteria (Magnavicula) penguinisozymeszymogram analysis
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 2 , August 2006 , pp. 127 - 133
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cai, YY, Zhang, Y and Wei, RF (1995) Summation of Conchology. Shanghai: Shanghai Science and Technology Press, pp. 209–210 (in Chinese).Google Scholar
Durand, P, Wada, KT and Komaru, A (1990) Triploidy induction by caffeine-heat shock treatments in the Japanese pearl oyster Pinctada fucada martensii. Nippon Suisan Gakkaishi 56(9): 1423–1425.CrossRefGoogle Scholar
Durand, P, Wada, KT and Blanc, F (1993) Genetics variation in wild and hatchery stock of the black pearl oyster, Pinctada margaritifera, from Japan. Aquaculture 110: 27–40.CrossRefGoogle Scholar
He, MX, Shen, Q and Lin, YG (2002) Comparison of heterozygosity and growth between diploid, triploid and aneuploid pearl oyster, Pinctada martensii (D.). Journal of Tropic Oceanology 21(4): 55–62 (in Chinese with English abstract).Google Scholar
Huang, Y (1998) Molecular Systematics—Principle, Method and its Application. Peking: Chinese Agriculture Press, pp. 258–271 (in Chinese).Google ScholarPubMed
Li, GL, Du, XD and Ye, FL (2001) Analysis on isozymes electrophoresis of Pincatada martensii. Journal of Fishery Sciences of China 8(2): 17–22 (in Chinese with English abstract).Google Scholar
Liang, FL, Mao, Y and Yu, XY (2001) A preliminary observation on the growth of the artificial spats of Pteria penguin. Journal of Zhanjiang Ocean University 21(1): 6–9 (in Chinese with English abstract).Google Scholar
Liu, BQ and Dai, JX (1998) Study on genetic diversity in Oysters crassotrea. Journal of Fisheries of China 22(3): 193–197 (in Chinese with English abstract).Google Scholar
Mao, SX and Xiang, H (2000). Genetic Ideology of Isozyme. Peking: Capical Normal University Press, pp. 12–26 (in Chinese).Google Scholar
Mao, Y, Liang, FL, Fu, S, Yu, XY, Ye, FL and Deng, CM (2004) Preliminary studies on rainbow-pearl of Pteria penguin. Chinese Journal of Zoology 39(1): 100–102 (in Chinese with English abstract).Google Scholar
Wang, AM, Yan, B, Lan, GB and Ye, L (2003) Comparison of three methods for the induction of tetraploidy in Pearl Oyster (Pinctada martensii D.). Journal of Agricultural Biotechnology 11(1): 64–69 (in Chinese with English abstract).Google Scholar
Wang, MF, Yu, XY, Liu, Y, Bao, LC and Feng, JH (2004) Comparison of mucous protein and isozymes from mantle tissue in two pearl oysters, Pinctada martensii Dunker and Pteria penguin Röding. Journal of Huazhong Agricultural University 23: 555–559 (in Chinese with English abstract).Google Scholar
Wang, ZR (1996) Plant Allozyme Analysis. Peking: Chinese Science Press, pp 14–36, 98-100, 140-144 (in Chinese).Google Scholar
Yu, XY, Wang, MF and Ye, FL (2000) Development and artificial propagation of Pteria penguin. Natural Science Journal of Hainan University 18(3): 266–269 (in Chinese with English abstract).Google Scholar
Yu, XY, Wang, MF, Liu, Y, Liang, FL, Mao, Y and Gui, JF (2004) The peculiarities of zymogram and their genetic analysis in Pteria penguin. Journal of Fisheries of China 28(4): 375–383 (in Chinese with English abstract).Google Scholar
Yu, XY, Wang, MF, Liang, FL and Liu, Y (2005) Differentiation of isozymes from different tissues of Pteria penguin Röding. Journal of Fishery Science of China 12: 201–206 (in Chinese with English abstract).Google Scholar
Zhu, LF (1992) Gradient gel electrophoresis on polyacrylamide of isozymes and proteins of fishes. Acta Hydrobiologica Sinica 16 (2): 183–185 (in Chinese).Google Scholar