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The genetic evaluation of crossbred pig populations for heterosis research by microsatellite markers

Published online by Cambridge University Press:  20 March 2007

Zhang Jing-Hu ,
Xiong Yuan-Zhu ,
Deng Chang-Yan ,
Jiang Si-Wen ,
Lei Ming-Gang ,
Zuo Bo ,
Xu De-Qun ,
Li Jia-Lian ,
Li Feng-E  and
Zheng Rong
Zhang Jing-Hu
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China Department of Biology, Science and Technology, Zhangzhou Normal University, Zhangzhou 363000, China
Xiong Yuan-Zhu*
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Deng Chang-Yan
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Jiang Si-Wen
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Lei Ming-Gang
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Zuo Bo
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Xu De-Qun
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Li Jia-Lian
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Li Feng-E
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Zheng Rong
Affiliation:
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. E-mail: xiongyzh@public.wh.hb.cn
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Abstract

The molecular genetic characteristics were evaluated, and the genetic effects of marker loci on heterosis of three traits (birth weight, BWT; average daily gain, ADG; and feed and meat ratio, FMR) were analysed in the experimental pig populations: Yorkshire (Y, n=34), Landrace (L, n=46) and Meishan (M, n=55); Yorkshire×Landrace (YL, n=32) and its reciprocal (LY, n=36), Yorkshire×Meishan (YM, n=82) and its reciprocal (MY, n=47), by 39 microsatellite markers selected from pig chromosomes SSC4, 6, 7, 8 and 13. The results indicated that observed alleles ranged from 2 to 6, average 4.13, observed heterozygosity varied from 0.39 (Y) to 0.58 (YM+MY) and average polymorphism information content (PIC) varied from 0.33 (Y) to 0.5 (YM). There were two loci (sw2155 and sw1037) at which the alleles were fixed in Y and L, and there were three loci (sw2409, sw2454 and sw1691) at which the alleles were fixed in M. The results from heterozygosity and kinship analysis revealed an intrinsic genetic relationship among the seven populations. Furthermore, the results on genetic effect analysis indicate that several marker loci had a significant effect on heterosis of the three traits in the two different F1 crossbred populations (P⩽0.01), for example s0161, swr1130 and sw1856 for BWT, sw1856 and swr2036 for ADG, and sw1302 and swr2036 for FMR. The significant marker loci implied a deep genetic relationship between molecular marker loci and heterosis.

Keywords

pigmicrosatellite markerheterozygosityheterosissignificant marker loci
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 3 , December 2006 , pp. 243 - 248
Copyright
China Agricultural University and Cambridge University Press 2006

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