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Identification of polymorphism in the goat callipyge gene (CLPG) and its associations with production traits

Published online by Cambridge University Press:  29 January 2010

Cao Gui-Ling ,
Li Biao ,
Tang Hui ,
Tang Pei-Rong ,
Wang Jian-Min  and
Jiang Yun-Liang
Cao Gui-Ling
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Li Biao
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Tang Hui
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Tang Pei-Rong
Affiliation:
Livestock Improvement Station of Alashan, Alashan Zuoqi, Inner Mongolia Autonomous Region, 750300, China
Wang Jian-Min
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Jiang Yun-Liang*
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
*
*Corresponding author. E-mail: yljiang723@yahoo.com.cn
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Abstract

The Dorset ram of the callipyge phenotype presents with muscular hypertrophy in the buttocks, and its inheritance is polar overdominant. A partial DNA fragment of 250 bp was obtained from the goat (Capra hircas) callipyge gene (CLPG; GenBank accession no. EU753362), which shared 96.04% and 88.65% identity with the corresponding regions of ovine (Ovis aries) and porcine CLPG, respectively. A polymorphism in the DNA fragment was detected by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Sequencing results indicated no A→C mutation corresponding to the ovine CLPG gene, although one A→C transversion was located 147 bp downstream from the CLPG site. The polymorphism, named SNP216 after its position (where SNP indicates single-nucleotide polymorphism), was investigated in Boer (n=63), Laiwu Black (n=70), Lubei White×Boer Hybrid (n=40), Lubei White (n=29) and Inner Mongolia Alashan White cashmere (n=115) goat populations. The results indicated that allele A was dominant in four of the goat populations, the Inner Mongolia Alashan White cashmere goats being the exception. The first four populations were in a state of Hardy–Weinberg equilibrium (P>0.05). In Inner Mongolia Alashan White cashmere goats, least-square means of birth weight, production of cashmere and body weight gain from birth to weaning did not differ significantly between the AA and AC phenotypes (P>0.5).

Keywords

goatCLPGpolymorphismproduction traits
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 235 - 240
Copyright
Copyright © China Agricultural University 2009

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