Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-10T09:11:08.746Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of polymorphism in the goat callipyge gene (CLPG) and its associations with production traits

Published online by Cambridge University Press:  29 January 2010

Cao Gui-Ling ,
Li Biao ,
Tang Hui ,
Tang Pei-Rong ,
Wang Jian-Min  and
Jiang Yun-Liang
Cao Gui-Ling
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Li Biao
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Tang Hui
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Tang Pei-Rong
Affiliation:
Livestock Improvement Station of Alashan, Alashan Zuoqi, Inner Mongolia Autonomous Region, 750300, China
Wang Jian-Min
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Jiang Yun-Liang*
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
*
*Corresponding author. E-mail: yljiang723@yahoo.com.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The Dorset ram of the callipyge phenotype presents with muscular hypertrophy in the buttocks, and its inheritance is polar overdominant. A partial DNA fragment of 250 bp was obtained from the goat (Capra hircas) callipyge gene (CLPG; GenBank accession no. EU753362), which shared 96.04% and 88.65% identity with the corresponding regions of ovine (Ovis aries) and porcine CLPG, respectively. A polymorphism in the DNA fragment was detected by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Sequencing results indicated no A→C mutation corresponding to the ovine CLPG gene, although one A→C transversion was located 147 bp downstream from the CLPG site. The polymorphism, named SNP216 after its position (where SNP indicates single-nucleotide polymorphism), was investigated in Boer (n=63), Laiwu Black (n=70), Lubei White×Boer Hybrid (n=40), Lubei White (n=29) and Inner Mongolia Alashan White cashmere (n=115) goat populations. The results indicated that allele A was dominant in four of the goat populations, the Inner Mongolia Alashan White cashmere goats being the exception. The first four populations were in a state of Hardy–Weinberg equilibrium (P>0.05). In Inner Mongolia Alashan White cashmere goats, least-square means of birth weight, production of cashmere and body weight gain from birth to weaning did not differ significantly between the AA and AC phenotypes (P>0.5).

Keywords

goatCLPGpolymorphismproduction traits
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 235 - 240
Copyright
Copyright © China Agricultural University 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdulkhaliq, AM, Meyer, HH, Thompson, JM, Holmes, ZA, Forsberg, NE and Davis, SL (2002) Callipyge gene effects on lamb growth, carcass traits, muscle weights and meat characteristics. Small Ruminant Research 45: 89–93.Google Scholar
Cockett, NE, Jackson, SP, Shay, TL, et al. (1994) Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. Proceedings of the National Academy of Sciences of the USA 91: 3019–3023.Google Scholar
Cockett, NE, Jackson, SP, Snowder, GD, et al. (1999) The callipyge phenomenon: evidence for unusual genetic inheritance. Journal of Animal Science 77: 221–227.Google Scholar
Fahrenkrug, SC, Freking, BA, Rexroad, CE, Leymaster, KA, Kappes, SM and Smith, TPL (2000) Comparative mapping of the ovine CLPG locus. Mammalian Genome 11: 871–876.Google Scholar
Freking, BA, Keele, JW, Beattie, CW, et al. (1998) Evaluation of the ovine callipyge locus: I. Relative chromosomal position and gene action. Journal of Animal Science 76: 2062–2071.Google Scholar
Freking, BA, Murphy, SK, Wylie, AA, et al. (2002) Identification of the single base change causing the callipyge muscle hypertrophy phenotype, the only known example of polar overdominance in mammals. Genome Research 12: 1496–1506.Google Scholar
Koohmaraie, M, Shackelford, SD, Wheeler, TL, Lonergan, SM and Doumit, ME (1995) A muscle hypertrophy condition in lamb (callipyge): characterization of effects on muscle growth and meat quality traits. Journal of Animal Science 73: 3596–3607.Google Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual 2nd edn.New York: Cold Spring Harbor Laboratory Press.Google Scholar
Shay, TL, Berghmans, S, Segers, K, et al. (2001) Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus. Mammalian Genome 12: 141–149.Google Scholar
Smit, M, Segers, K, Carrascosa, LG, et al. (2003) Mosaicism of Solid Gold supports the causality of a noncoding A-to-G transition in the determinism of the callipyge phenotype. Genetics 163: 453–456.Google Scholar
Wang, HL, Li, XL, Zhou, RY, Li, LH and Guo, XL (2007) Detection of goat callipyge genotype. China Animal Husbandry & Veterinary Medicine 34: 49–51 (in Chinese with English abstract).Google Scholar
Wu, J, Shi, HC, Zhang, ZF and Jian, ZJ (2006) Detection of sheep callipyge genotype. Journal of Animal Science and Veterinary Medicine 25: 1–4 (in Chinese).Google Scholar