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Isolation and analysis of genes induced by rehydration after serious drought in broomcorn millet (Panicum miliaceum L.) by SSH

Published online by Cambridge University Press:  20 March 2007

Lin Fan-Yun ,
Hu Yin-Gang ,
Song Guo-Qi ,
Zhang Hong ,
Liu Tian-Ming  and
He Bei-Ru
Lin Fan-Yun
Affiliation:
College of Agronomy, Northwest A & F University, Yangling 712100, China
Hu Yin-Gang*
Affiliation:
College of Agronomy, Northwest A & F University, Yangling 712100, China Yangling Branch of China National Wheat Improvement Center, Northwest A & F University, Yangling 712100, China Shaanxi Key Lab of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China
Song Guo-Qi
Affiliation:
College of Agronomy, Northwest A & F University, Yangling 712100, China
Zhang Hong
Affiliation:
College of Agronomy, Northwest A & F University, Yangling 712100, China Yangling Branch of China National Wheat Improvement Center, Northwest A & F University, Yangling 712100, China
Liu Tian-Ming
Affiliation:
College of Agronomy, Northwest A & F University, Yangling 712100, China
He Bei-Ru
Affiliation:
College of Agronomy, Northwest A & F University, Yangling 712100, China Yangling Branch of China National Wheat Improvement Center, Northwest A & F University, Yangling 712100, China
*
*Corresponding author. E-mail: huyingang8126@yahoo.com
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Abstract

In order to investigate the molecular mechanism of rehydration after serious drought in broomcorn millet (Panicum miliaceum L.), a forward subtracted cDNA library was constructed between normal watered leaves and rehydrated leaves after serious drought conditions, using the suppressive subtraction hybridization (SSH) technique. A total of 60 positive clones were picked out at random from the subtracted library and sequenced, and redundancy sequences were removed after sequence alignment. Based on the results of sequence homologous comparison and function querying, 32 expressed sequence tags (EST) were highly homologous with known ESTs. Most of those sequences were related to either abiotic or biotic stress in plants. Of those sequences, 11 ESTs were homologous with ESTs in rat (Rattus norvegicus) liver after partial hepatectomy. The Blast result of proteins revealed that 28 ESTs were similar to known proteins. The functions of these proteins mainly involve signal transduction, transcription and protein processing. This experiment demonstrated that a range of specific genes was induced and expressed in broomcorn millet during the rehydration stage after serious drought.

Keywords

broomcorn milletdrought stressrehydrationgene expressionsuppressive subtraction hybridization
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 3 , December 2006 , pp. 237 - 242
Copyright
China Agricultural University and Cambridge University Press 2006

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References

Bernacchia, G, Salamini, F and Bartels, D (1996) Molecular characterization of the rehydration process in the resurrection pIant Craterostigma plantagineum. Plant Physiology 111: 1043–1050.CrossRefGoogle Scholar
Bush, DS (1995) Calcium regulation in plant cells and its role in signaling. Annual Review of Plant Physiology and Plant Molecular Biology 46: 95–122.CrossRefGoogle Scholar
Diatchenko, L, Lauy, FC, Campbell, AP, et al. (1996) Suppression subtractive hybridization: a method for generating differentially regulated or tissue specific cDNA probes and libraries. Proceedings of National Academy Sciences of the USA 93(12):6025–6030.Google Scholar
Gorantla, M, Babu, PR, Lachagari, VBR, Feltus, FA, Paterson, AH and Reddy, AR (2005) Functional genomics of drought stress response in rice: Transcript mapping of annotated unigene of an indica rice (Oryza sativa L. cv. Nagina 22). Current Science 89(3):496–517.Google Scholar
Liu, GF, Hou, YJ, Wang, YC and Chu, YG (2005) Construction and analysis of Tamarix hispida suppression subtractive hybridization library under drought stress. Bulletin of Botanical Research 25(1):69–73 (in Chinese with English abstract).Google Scholar
Liu, Q, Zhang, GY and Chen, SY (2000a) The structures and regulation functions of plant transcription factors. Chinese Science Bulletin 45(14):1465–1474 (in Chinese).Google Scholar
Liu, Q, Zhang, Y and Chen, SY (2000b) Plant protein kinase genes induced by drought, high-salt and low-temperature. Chinese Science Bulletin 45(6):561–566 (in Chinese).Google Scholar
Oono, Y, Seki, M and Nanjo, T (2003) Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca. 7000 full-length cDNA microarray. The Plant Journal 34(6): 868–887.CrossRefGoogle ScholarPubMed
Reichman, TW, Muñiz, LC and Mathews, MB (2002) The RNA binding protein nuclear factor 90 functions as both a positive and negative regulator of gene expression in mammalian cells. Molecular and Cellular Biology 22(1): 343–356.Google Scholar
Seki, M, Narusaka, M, Abe, H, et al. (2001) Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell 13: 61–72.CrossRefGoogle ScholarPubMed
Seki, M, Narusaka, M, Ishida, J, et al. (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. The Plant Journal 31(3): 279–292.Google Scholar
Tezara, W, Mitchell, VJ, Driscoll, SD and Lawlor, DW (1999) Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. Nature 401: 914–917.CrossRefGoogle Scholar
Theisen, AA, Knox, EG and Mann, FL (1978) Feasibility of Introducing Food Crops Better Adapted to Environmental Stress. Vol. II. Individual Crop Reports. Washington, DC:National Science Foundation, pp. 168–172.Google Scholar
Wang, Z, Zang, QW, Guo, ZA and Jing, RL (2004) A preliminary study on gene expression profile induced by water stress in wheat (Triticum aestivum L.) seedling. Acta Genetica Sinica 31(8): 842–849 (in Chinese with English abstract).Google Scholar
Yu, J, Ma, KT and Zhang, H (1998) The multiple functions of molecular chaperones. Progress In Biochemistry and Biophysics 25(1): 106–110 (in Chinese with English abstract).Google Scholar
Zheng, J, Zhao, JF, Tao, YZ, et al. (2004) Isolation and analysis of water stress induced genes in maize seedlings by subtractive PCR and cDNA macroarray. Plant Molecular Biology 55: 807–823.CrossRefGoogle ScholarPubMed