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Isolation and sequence analysis of the floral homeotic gene BAP2 in Brassica rapa

Published online by Cambridge University Press:  12 February 2007

Lu Guang-Yuan ,
Wu Xiao-Ming ,
Chen Bi-Yun ,
Gao Gui-Zhen  and
Xu Kun
Lu Guang-Yuan
Affiliation:
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Wu Xiao-Ming*
Affiliation:
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Chen Bi-Yun
Affiliation:
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Gao Gui-Zhen
Affiliation:
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Xu Kun
Affiliation:
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
*
*Corresponding author. Email: wuxm@oilcrops.cn
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Abstract

The Arabidopsis homeotic gene AP2 is essential for floral meristem development and organ specification. In this study, we report the isolation and sequence comparison of the AP2 homologue of Brassica rapa (BAP2). The results showed that the BAP2 DNA sequence was 2138 bp in length and contained nine introns. It shared 90% identity with AP2 in the coding region. The putative BAP2 polypeptide contained a nuclear localization signal (NLS) sequence and two copies of highly conserved AP2 domain, suggesting that BAP2 may function similarly to AP2. Two nucleotide variations were detected in DNA sequences from wild-type and apetalous B. rapa plants at the BAP2 locus, while the putative polypeptides were identical. We propose that BAP2 is not likely to be responsible for the apetalous mutation in B. rapa.

Keywords

apetalous mutationBrassica rapahomeotic geneTAIL-PCR
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 2 , August 2006 , pp. 141 - 146
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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