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Karyotyping of Brassica oleracea C genome using Brassica A genomic DNA as blocking agent

Published online by Cambridge University Press:  29 January 2010

Chen Xiao-Dan ,
Zhu Li-Quan ,
Wang Yong ,
Rong Xiao-Ying ,
Lu Jun  and
Wang Xiao-Jia
Chen Xiao-Dan
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Zhu Li-Quan*
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Wang Yong
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Rong Xiao-Ying
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Lu Jun
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Wang Xiao-Jia
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
*
*Corresponding author. E-mail: zhuliquan@swu.edu.cn
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Abstract

In exploring an effective and reliable karyotyping method in Brassica crop plants, Brassica oleracea was successfully karyotyped using genomic in situ hybridization (GISH). B. oleracea genomic DNA was labelled as probe using DIG-high prime mix kit, with B. rapa genomic DNA acting as blocking agent. Specific fluorescent signals were detected on each pair of homologous chromosomes, and nine pairs of chromosomes of B. oleracea were clearly identified according to the signal characteristics. A practical and accurate method for conducting karyotyping of small chromosomes has been demonstrated.

Keywords

Brassica oleraceaBrassica rapagenomic in situ hybridizationkaryotype analysis
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 227 - 233
Copyright
Copyright © China Agricultural University 2009

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