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Mapping, tissue distribution and polymorphism of porcine genes encoding retinol binding proteins (RBP1 and RBP4)

Published online by Cambridge University Press:  27 June 2008

Gong Wei-Hua
Affiliation:
Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Tang Zhong-Lin
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Cui Wen-Tao
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Yang Shu-Lin
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Li Kui*
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
*
*Corresponding author. E-mail: likuihau@yahoo.com

Abstract

Using the INRA – University of Minnesota porcine 7000 Rad Radiation Hybrid panel (IMpRH) containing 118 clones, porcine cellular retinol binding protein gene 1 (RBP1) and plasma retinol binding protein gene 4 (RBP4) were assigned to porcine chromosomes 13 and 14, respectively. The mRNA distributions of the two genes in adult Wuzhishan pig tissues (lung, skeletal muscle, spleen, heart, stomach, large intestine, lymph node, small intestine, liver, brain, kidney and fat) were examined. One single nucleotide polymorphism (SNP) was identified in Laiwu, Wuzhishan, Guizhou, Bama pig breeds and a Tongcheng experimental population and analysed using a polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method. Significant associations were found between different genotypes of A/G156 and maximum carcass length (LM), minimum carcass length (LN), haemoglobin (HGB), mean corpuscular volume haemoglobin (MCH), and day to slaughter weight (age) traits in the Tongcheng experimental population. This SNP locus might be used as a genetic marker in pig breeding and production.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 729–734

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