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Obtaining transgenic maize plants expressing the rabbit defensin (NP-1) gene and evaluation of their disease resistance

Published online by Cambridge University Press:  12 February 2007

Zhang Wen-He ,
Zhao Qian ,
Yu Jing-Juan ,
Zhu Deng-Yun  and
Ao Guang-Ming
Zhang Wen-He
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Zhao Qian
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Yu Jing-Juan
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Zhu Deng-Yun
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Ao Guang-Ming*
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: aogm@cau.edu.cn
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Abstract

The rabbit defensin gene NP-1 was introduced into embryonic callus cultures of three maize (Zea mays L.) hybrid lines by particle bombardment, and transgenic plants were obtained. Genomic PCR and DNA dot blot analyses confirmed that the NP-1 gene was integrated into the genome of the regenerated T0 maize plants. Genomic PCR and Southern blotting results revealed that the NP-1 gene was transmitted stably from the T0 to the T1 generation. RNA dot blot analysis verified the transcription of the NP-1 gene in T1 plants. When challenged with northern corn leaf blight (Helminthosporium turcicum Pass), the T1 plants expressing the NP-1 gene showed greatly improved resistance to the fungal disease compared with the wild-type maize plants.

Keywords

rabbit defensin genemaizenorthern corn leaf blight
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 1 , April 2004 , pp. 55 - 59
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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References

Gritz, L and Davies, J (1983) Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179–188.Google Scholar
Leath, S and Pedersen, WL (1986) Differences in resistance between maize hybrids with or without the Ht1 gene when infected with Exserohilum turcicum race 2. Phytopathology 76: 257–260.CrossRefGoogle Scholar
Li, J-X (1992) The Study Advance of Maize Breeding. Beijing: Science Press (in Chinese).Google Scholar
Li, H-JHu, Z-L, Wei, H et al. , (2001) The study advance of defensins. Progress in Biotechnology 21: 34–37. (in Chinese with English abstract)Google Scholar
Lyer, LM, Kumpatla, SP, Chandrasekharan, MB and Hail, TC (2000) Transgene silencing in monocots. Plant Molecular Biology 43: 323–346.Google Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Terras, FRG, Torrekens, S, van Leuven, F et al. , (1995) Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7: 573–588.Google Scholar
Wang, G-Y, Du, T-B, Zhang, H et al. ,(1995) Transfer of Bt gene into maize by microprojectile bombardment and regeneration of transgenic plants. Science in China. Series B, Chemistry, Life Sciences & Earth Sciences 25: 71–76.Google Scholar
Wang, Z-X (1999) Shandong Maize. Beijing: China Agricultural Press (in Chinese).Google Scholar
Zhang, M-C and Zheng, G-C (1997) Human defensin-1 ( HNP-1 ) expressed in tobacco: a preliminary observation on anti-TMV activity of the transgenic tobacco plants. Acta Botanica Sinica 39: 574–576. (in Chinese with English abstract)Google Scholar