Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-28T04:45:44.242Z Has data issue: false hasContentIssue false
  • English
  • Français

RAPD analysis on genetic diversity of typical tea populations in Hunan Province

Published online by Cambridge University Press:  27 June 2008

Shen Cheng-Wen ,
Huang Yi-Huan ,
Huang Jian-An ,
Luo Jun-Wu ,
Liu Chun-Lin  and
Liu De-Hua
Shen Cheng-Wen
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China College of Horticulture and Gardening of Hunan Agricultural University, Changsha 410128, China
Huang Yi-Huan*
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China
Huang Jian-An
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China
Luo Jun-Wu
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China College of Horticulture and Gardening of Hunan Agricultural University, Changsha 410128, China
Liu Chun-Lin
Affiliation:
Crop Gene Engineering Key Laboratory of Hunan Province, Changsha 410128, China
Liu De-Hua
Affiliation:
College of Horticulture and Gardening of Hunan Agricultural University, Changsha 410128, China
*
*Corresponding author. E-mail: scw69@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Genetic diversity and genetic variation of 240 adult plants of four tea populations in Hunan – Camellia sinensis var. sinensis, C. sinensis var. assamica cv. Duntsa, C. ptilophylla and C. sinensis var. assamica cv. Jianghua – were studied by rapid amplification of polymorphic DNA (RAPD) markers. The results showed 226 loci using 21 random primers (10 bp), of which 201 (88.9%) were polymorphic. The genetic diversity analysis indicated that Shannon's index was 0.43; 74.7% of which was within-population genetic diversity while 25.3% was among-population variation. The gene diversity indexes of total populations (HT), within populations (HS) and among populations (HST) were, respectively, 0.37, 0.28 and 0.09. The coefficient of gene differentiation (GST) among populations was 0.23, indicating a 76.7% variation within populations and 23.3% among populations. These results displayed a rich within-population genetic variation, as in Shannon's diversity index. Interpopulation gene flow (Nm) was 0.74, which indicates the limited genetic exchange between populations.

Keywords

Camellia sinensisRAPD analysispopulationgenetic diversity
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 67 - 72
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 855–860

References

Caro, TM and Laurenson, MK (1994) Ecological and genetic factors in conservation. Science 263: 485–486.CrossRefGoogle ScholarPubMed
Chen, XY, Chen, GB, Tang, MD, Qu, WQ and Li, J (1989) Study on community idioplasm resources in Hunan Province. Tea Communication (1): 34–39.Google Scholar
Chen, YJ, Wang, W, Yang, YX, et al. (1997) Genetic divergence of Cordyceps sinensis as estimated by random amplified polymorphic DNA analysis. Acta Genetica Sinica 24(5): 410–416.Google ScholarPubMed
Huang, JA, Huang, YH, Luo, JW, Wang, KB and Zhou, LH (2003) Efficient methods for genomic DNA extraction from Camellia sinensis. Journal of Hunan Agricultural University (Natural Sciences) 29(5): 402–407.Google Scholar
Li, JM, Jin, ZX and Zhong, ZC (2004) RAPD analysis of genetic diversity of Sargentodoxa cuneata at different altitude and the influence of environmental factors. Acta Ecologica Sinica 24(3): 567–573.Google Scholar
Li, J, Jiang, CJ and Wang, ZX (2005) RAPD analysis on genetic diversity of the preconcentrated core germplasms of Camellia sinensis in China. Hereditas (Beijing) 27(5): 765–771.Google ScholarPubMed
Li, XH, Hu, MF, Liu, FZ, Liu, ZH, Cai, LY and Zhang, LX (1997) A study of native tea resources in Hunan Province. Journal of Hunan Agricultural University (Natural Sciences) 23(6): 543–547.Google Scholar
Luo, JW, Shi, ZP, Shen, CW, Liu, CL, Gong, ZH and Huang, YH (2004) The genetic diversity of tea germplasms [Camellia sinensis (L.) O. Kuntze] by RAPD analysis. Acta Agronomica Sinica 30(3): 266–269.Google Scholar
Moritz, C (1994) Defining ‘evolutionary significant units’ for conservation. Tree 9: 373–375.Google Scholar
Nei, M (1987) Molecular Evolutionary Genetics. New York: Columbia University Press.CrossRefGoogle Scholar
Oh, MJ (1994) Studies on genetic relationship among the Korean native tea trees and physicochemical properties of its green tea. PhD Thesis, College of Agronomy, Gaoli University, Korea, pp. 27–29.Google Scholar
Pich, U and Schubert, I (1993) Midiprep method for isolation of DNA from plants with a high content of polyphenolics. Nucleic Acids Research 21: 3328–3332.CrossRefGoogle ScholarPubMed
Wang, XQ, Zou, YP, Zhang, DM, Zhang, ZX and Hong, DY (1996) Problems in the use of RAPD to the study of genetic diversity and systematics. Acta Botanica Sinica 38(12): 954–962.Google Scholar