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Simultaneous detection of bovine- and sheep-derived materials in feedstuffs by PCR

Published online by Cambridge University Press:  12 February 2007

Wen Wei-Gang ,
Zhao Xiu-Ling ,
He Shui-Shan ,
Du Hua-Hong  and
Huang Shao-Tang
Wen Wei-Gang*
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
Zhao Xiu-Ling
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
He Shui-Shan
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
Du Hua-Hong
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
Huang Shao-Tang
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
*
*Corresponding author: Email: wenwg@nbciq.gov.cn
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Abstract

To control the spread of bovine spongiform encephalopathy and scrapie, a method was developed for the simultaneous detection of bovine- and sheep-derived materials in feedstuffs. Chelex-100 was adopted for the fast extraction of animal genomic DNA. A pair of universal primers, designed on the basis of homology in sequences of bovine and sheep mtDNA, was used for PCR. This method is of great value to quarantine departments for its simplicity and rapid detection.

Keywords

Chelex-100PCRuniversal primersbovine- and sheep-derived materialssimultaneous detection
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 13 - 16
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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References

Anderson, RM, Donnelly, CA, Ferguson, NM, Woolhouse, MEJ, Watt, CJ, Udy, HJ, et al. (1996) Transmission dynamics and epidemiology of BSE in British cattle. Nature 382: 778–779.Google Scholar
Michaels, GS, Hauswirth, WW and Laipis, PJ (1988) Mitochondrial DNA copy number in bovine oocytes and somatic cells. Developmental Biology 94: 246–251.Google Scholar
Pan, L-W, Chen, J-H (2002) Development of detection of animal derived materials in food and feedstuff. Inspection and Quarantine Science 12 (2): 45–47 (in Chinese).Google Scholar
Robin, ED and Wong, R (1988) Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells. Journal of Cell Physiology 136: 507–513.CrossRefGoogle ScholarPubMed
Sambrook, J, Fritsch, EF and Maniatis, T (1999) Molecular Cloning: A Laboratory Manual, 2nd ed. Beijing: Science Press (in Chinese).Google Scholar
Tartaglia, M, Saulle, E, Pestalozza, S, Morelli, L, Antonucci, G and Battaglia, PA (1998) Detection of bovine mitochondrial DNA in ruminant feeds: a molecular approach to test for the presence of bovine-derived materials. Journal of Food Protection 61(5): 513–518.Google Scholar
Xu, B-L, Yang, B-H, Wang, Q, Chao, J-J and Bo, Q-R (2002) Identification of bovine, sheep and goat derived materials in imported animal derived feedstuff. SN/T 1119–2002. Beijing: China Standard Press (in Chinese and English).Google Scholar
Zheng, X-F (2002) DNA Analysis in Medical Jurisprudence. Beijing: China Police University Press (in Chinese).Google Scholar