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SSR markers for identification of purity of melon hybrids

Published online by Cambridge University Press:  03 March 2009

Li Ju-Fen ,
Ma Guo-Bin  and
Xu Ling
Li Ju-Fen
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
Ma Guo-Bin*
Affiliation:
Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201106, China
Xu Ling*
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
*
*Corresponding authors. E-mail: maguobin@sh163.net and lxu@bio.ecnu.edu.cn
*Corresponding authors. E-mail: maguobin@sh163.net and lxu@bio.ecnu.edu.cn
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Abstract

The hybrid purity of melon (Cucumis melo L.) was tested by polymerase chain reaction (PCR) assay based on simple sequence repeat (SSR) markers in two F1 melon hybrids (‘Dongfangmi 1’ and ‘Dongfangmi 2’) and their parental lines. Twelve pairs of SSR primers for ‘Dongfangmi 1’ and three pairs for ‘Dongfangmi 2’ were selected. Results showed that self-inbred seeds were effectively distinguished from F1 hybrid seeds using these SSR primers, a finding that was consistent with the results recorded from field tests. ‘Dongfangmi 1’ and ‘Dongfangmi 2’ were identified from their parental lines, and seven other uterine hybrid lines by multiplex primers MS48+MS60 and MS4+MS20, respectively. Contamination of F1 hybrid seeds caused by self-inbred and other unknown pollens can be effectively and more reliably detected by PCR assays with multiplex SSR primers.

Keywords

melon (Cucumis melo L.)SSR markerhybrid seed purity
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 223 - 229
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(3): 494–500

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