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HOW UNIVERSAL ARE UNIVERSAL rDNA PRIMERS? A CAUTIONARY NOTE FOR PLANT SYSTEMATISTS AND PHYLOGENETICISTS

Published online by Cambridge University Press:  04 December 2000

M. MÖLLER
M. MÖLLER
Affiliation:
Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, UK
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Abstract

Phylogenetic relationships are frequently inferred from rDNA ITS sequences obtained employing universal primers. Whereas in most cases phylogenetic topologies inferred from ITS data make good biological sense, caution has to be used when a number of taxa vary greatly in PCR yield. The phylogenetic implications of such a scenario are discussed.

Keywords

Internal transcribed spacernucleolar organizer regionsPCRribosomal DNAuniversal primers
Type
Research Article
Information
Edinburgh Journal of Botany , Volume 57 , Issue 2 , July 2000 , pp. 151 - 156
Copyright
© 2000 Trustees of the Royal Botanic Garden, Edinburgh

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