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INTROGRESSIVE HYBRIDIZATION BETWEEN RHODODENDRON KIUSIANUM AND R. KAEMPFERI (ERICACEAE) IN KYUSHU, JAPAN BASED ON CHLOROPLAST DNA MARKERS

Published online by Cambridge University Press:  01 November 2007

N. Kobayashi ,
T. Handa ,
I. Miyajima ,
K. Arisumi  and
K. Takayanagi
N. Kobayashi*
Affiliation:
Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki, 305-8572 Japan. Corresponding author (e-mail: nkobayashi@life.shimane-u.ac.jp). Present address: Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, 690-8504 Japan.
T. Handa
Affiliation:
Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki, 305-8572 Japan.
I. Miyajima
Affiliation:
Faculty of Agriculture, Kyusyu University, Fukuoka, 812-8581 Japan. Present address: Institute of Tropical Agriculture, Kyushu University, Fukuoka, 812-8581 Japan.
K. Arisumi
Affiliation:
Faculty of Agriculture, Kagoshima University, Kagoshima, 890-0065 Japan.
K. Takayanagi
Affiliation:
Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki, 305-8572 Japan.
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Abstract

Wild evergreen azalea populations of Rhododendron kiusianum and R. kaempferi (Ericaceae) were analysed using a chloroplast DNA (cpDNA) PCR-RFLP marker that was used to detect introgressive hybridization in our previous study of the Kirishima Mts populations.

The populations of the intermediate region in the Unzen Mts, which show phenotypic variation, were demonstrated to result from interspecific hybridization between Rhododendron kiusianum and R. kaempferi, possessing cpDNA from either R. kiusianum (1030/420 bp) or R. kaempferi (950/420/80 bp).

Most individuals of Rhododendron kiusianum in the Kujyu Mts, the Aso Mts and the surrounding mountains exhibited the PCR-RFLP pattern of R. kaempferi. These results from the Kujyu Mts and the Aso Mts indicate that natural hybridization and cytoplasmic introgression from Rhododendron kaempferi to R. kiusianum have occurred in the relatively distant past. In the case of Mt Yufudake and Mt Haneyama, the Rhododendron kiusianum population retains the effects of natural hybridization with R. kaempferi in the cpDNA as well as in the variation in flower characteristics.

All individuals of Rhododendron kiusianum on Mt Onogaradake in the Takakuma Mts exhibit R. kiusianum cpDNA (1030/420 bp), in spite of variation in flower colour.

Keywords

Chloroplast DNA variationintrogressive hybridizationRhododendron kaempferiRhododendron kiusianum
Type
Articles
Information
Edinburgh Journal of Botany , Volume 64 , Issue 3 , November 2007 , pp. 283 - 293
Copyright
Copyright © Trustees of the Royal Botanic Garden, Edinburgh 2007

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References

Aburaya, K., Yamada, S. & Noda, M. (1979). The natural variation of Rhododendron kiusianum in Mt Unzen. Res. Rep. Agri. Forest Ins. Nagasaki Pref. 7: 227–250(in Japanese).Google Scholar
Arnold, M. L., Buckner, C. M. & Robinson, J. J. (1991). Pollen-mediated introgression and hybrid speciation in Louisiana irises. Proc. Natl. Acad. Sci. U.S.A. 88: 1398–1402.CrossRefGoogle ScholarPubMed
Kobayashi, N., Horikoshi, T., Katsuyama, H., Handa, T. & Takayanagi, K. (1998). A simple and efficient DNA extraction method from plants, especially from woody plants. Pl. Tissue Cult. Biotechnol. 4: 76–80.Google Scholar
Kobayashi, N., Handa, T., Yoshimura, K., Tsumura, Y., Arisumi, K. & Takayanagi, K. (2000). Evidence for introgressive hybridization based on chloroplast DNA polymorphisms and morphological variation in wild evergreen azalea populations in the Kirishima Mountains, Japan. Edinburgh J. Bot. 57: 209–219.Google Scholar
Kron, K. A., Gawen, L. M. & Chase, M. W. (1993). Evidence for introgression in azaleas (Rhododendron: Ericaceae): chloroplast DNA and morphological variation in a hybrid swarm on Stone Mountain, Georgia. Amer. J. Bot. 80: 1095–1099.Google Scholar
Kunishige, M. & Kobayashi, Y. (1980). Chromatographic identification of Japanese azalea species and their hybrids. In: Luteyn, J. L. & O'Brien, M. E. (eds) Contributions toward a classification of Rhododendron, pp. 277–287. New York: New York Botanical Garden.Google Scholar
Kunishige, M. & Tamura, T. (1961). The natural variation of Rhododendron kaempferi on Mt Kirishima. Kyushu. Agric. Res. 23: 157–158(in Japanese).Google Scholar
Miyajima, I., Uemoto, S., Sakata, Y. & Arisumi, K. (1995). Morphological and pigment variations in flowers of Rhododendron kiusianum Makino and R. kaempferi Planch. indigenous to the Unzen Mountain Mass. J. Jap. Soc. Hort. Sci. 64: 393–399(in Japanese).CrossRefGoogle Scholar
Miyajima, I., Uemoto, S., Sakata, Y. & Arisumi, K. (1997). Morphology and flower pigments of wild evergreen azaleas (Rhododendron sataense Nakai) in southern Kyushu. J. Jap. Soc. Hort. Sci. 66: 385–391.CrossRefGoogle Scholar
Miyajima, I., Kurose, K., Matsuda, S., Uemoto, S. & Sakata, Y. (2001). Variations in flower morphology and pigments in Rhododendron kiusianum Makino endemic to Kuju and Aso mountains and surrounding areas. J. Jap. Soc. Hort. Sci. 70: 108–114(in Japanese).Google Scholar
Sakata, Y., Miyajima, I. & Arisumi, K. (1991). Some morphological and pigmental characteristics in Rhododendron kaempferi Planch., R. kiusianum Makino and R. eriocarpum Nakai in southern Kyushu. J. Jap. Soc. Hort. Sci. 60: 669–675.CrossRefGoogle Scholar
Sakata, Y., Miyajima, I. & Arisumi, K. (1993). Variations in some morphological and pigmental characteristics in Rhododendron kaempferi Planch., R. kiusianum Makino and their natural hybrids on Kirishima mountain mass. J. Jap. Soc. Hort. Sci. 61: 925–932.Google Scholar
Shinozaki, K., Ohme, M., Tanaka, M., Wakasugi, T., Hayashida, N., Matsubayashi, T. et al. (1986). The complete nucleotide sequence of the tobacco chloroplast genome, its gene organization and expression. EMBO J. 5: 2043–2049.Google Scholar
Yamazaki, T. (1996). A revision of the genus Rhododendron in Japan, Taiwan, Korea, and Sakhalin. Tokyo: Tsumura Laboratory.Google Scholar
Yokogawa, M. & Hotta, M. (1995). Taxonomical notes on plants of southern Japan II. Variation in the Rhododendron obtusum group in the Kirisima Mts., southern Kyushu. Acta Phytotax. Geobot. 46: 165–183.Google Scholar