Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-27T12:42:52.445Z Has data issue: false hasContentIssue false
  • English
  • Français

A comparative investigation of the metabolism of the herbicide glufosinate in cell cultures of transgenic glufosinate-resistant and non-transgenic oilseed rape (Brassica napus) and corn (Zea mays)

Published online by Cambridge University Press:  15 October 2002

Monika Ruhland ,
Gabriele Engelhardt  and
Karlheinz Pawlizki
Monika Ruhland
Affiliation:
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Außenstelle München, Menzinger Straße 54, 80638 München, Germany
Gabriele Engelhardt
Affiliation:
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Außenstelle München, Menzinger Straße 54, 80638 München, Germany
Karlheinz Pawlizki
Affiliation:
Bayerische Landesanstalt für Bodenkultur und Pflanzenbau, Menzinger Straße 54, 80638 München, Germany

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

To obtain information on differences between the metabolic pathways of the herbicide glufosinate (trade names: BASTA®, LIBERTY®) in non-transgenic, glufosinate-sensitive plants and in transgenic, glufosinate-resistant plants, the metabolism of 14C-labeled glufosinate and its enantiomers L- and D-glufosinate was studied using cell cultures of oilseed rape and corn. Transformation of glufosinate in both sensitive and transgenic rape cells remained at a low rate of about 3-10% in contrast to corn cells, where 20% was transformed in sensitive and 43% in transgenic cells after 14 days of incubation, the rest remaining as unchanged glufosinate. In sensitive rape and corn cells the main metabolite was 4-methylphosphinico-2-oxo-butanoic acid (PPO) with 7.3 and 16.4%, respectively, together with low amounts of 3-methylphosphinicopropionic acid (MPP), 4-methylphosphinico-2-hydroxybutanoic acid (MHB), 4-methylphosphinicobutanoic acid (MPB) and 2-methylphosphinicoacetic acid (MPA). An additional metabolite formed in transgenic cell cultures was 2-acetamido-4-methylbutanoic acid (N-acetyl-L-glufosinate, NGA), which was formed at rates of 3.2% in rape and 16.1% in corn. A further minor metabolite, not yet identified, was detected in both cell types. The liberation of 0.2% 14CO2 indicates further metabolic steps prior to a limited mineralization in plant cell cultures. L-glufosinate was transformed into the same metabolites as the glufosinate racemate. D-glufosinate was not metabolized.

Keywords

glufosinateherbicide resistancemetabolismphosphinothricintransgenic plantscell cultures
Type
Research Article
Information
Environmental Biosafety Research , Volume 1 , Issue 1 , October 2002 , pp. 29 - 37
Copyright
© ISBR, EDP Sciences, 2002

References

Behrendt, H, Matthies, M, Gildemeister, H, Görlitz, G (1990) Leaching and transformation of glufosinate-ammonium and its main metabolite in a layered column. Environ. Toxicol. Chem. 9: 541-549 CrossRef
Dröge, W, Broer, I, Pühler, A (1992) Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide L-phosphinothricin (glufosinate) differently from untransformed plants. Planta 187: 142-151 CrossRef
Dröge-Laser, W, Siemeling, U, Pühler, A, Broer, I (1994) The metabolites of the herbicide L-phosphinothricin (glufosinate) - identification, stability, and mobility in transgenic, herbicide-resistant, and untransformed plants. Plant Physiol. 105: 159-166 CrossRef
Gamborg, OL, Miller, RA, Ojima, K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158 CrossRef
Jansen, C, Schuphan, I, Schmidt, B (2000) Glufosinate metabolism in excised shoots and leaves of twenty plant species. Weed Science 48: 319-326 CrossRef
Komoßa, D, Sandermann, H jr (1992) Plant metabolism of herbicides with C-P Bonds: phosphinothricin. Pestic. Biochem. Physiol. 43: 95-102 CrossRef
Langebartels, C, Harms, H (1986) Plant cell suspension cultures as test systems for an ecotoxicological evaluation of chemicals. Angew. Botanik 60: 113-123
Lea, PJ, Joy, KW, Ramos, JL, Guerrero, MG (1984) The action of the 2-amino-4-(methylphosphinyl)-butanoic acid (phosphinothricin) and its 2-oxo-derivative on the metabolism of cyanobacteria and higher plants. Phytochemistry 23: 1-6 CrossRef
Leason, M, Cunliffe, D, Parkin, D, Lea, PJ, Miflin, B (1982) Inhibition of pea leaf glutamine synthetase by methioninsulfoximine. Phosphinothricin and other glutamate analogs. J. Phytochem. 21: 855-857 CrossRef
Müller B, Schuphan I, Schmidt B (1999) Metabolismus von Glufosinate in Zellkulturen verschiedener Pflanzenspezies (transgen, nicht-transgen) - Probleme bei Versuchsdesign, Aufarbeitung und Analytik, Mitteilungen der Deutschen Phytomedizinischen Gesellschaft 29: 12-13
Müller, B, Zumdick, A, Schuphan, I, Schmidt, B (2001) Metabolism of the herbicide glufosinate-ammonium in plant cell cultures of transgenic (rhizomania-resistant) and non-transgenic sugar beet (Beta vulgaris), carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). Pest Manag. Sci. 57: 46-56 3.0.CO;2-1>CrossRef
Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-477 CrossRef
Wohlleben W, Arnold W, Broer I, Hillemann D, Strauch E, Pühler A (1988) Nucleotide sequence of the phosphinothricin-N-acetyl-transferase gene from Streptomyces viridochromogenes Tü 494 and its expression in Nicotiana tabacum. Gene 70: 25-37 CrossRef
Zumdick A, Goerlitz G, Erzgräber B, Stumpf K, Dorn E (1998) Glufosinate-ammonium and N-acetyl-glufosinate: Soil metabolism. 9th Int. Congress of Pesticide Chemistry, London