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Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides

Published online by Cambridge University Press:  22 June 2006

Hikaru Saji ,
Nobuyoshi Nakajima ,
Mitsuko Aono ,
Masanori Tamaoki ,
Akihiro Kubo ,
Seiji Wakiyama ,
Yoriko Hatase  and
Masato Nagatsu
Hikaru Saji
Affiliation:
 Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Nobuyoshi Nakajima
Affiliation:
 Biodiversity Conservation Research Project, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Mitsuko Aono
Affiliation:
 Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Masanori Tamaoki
Affiliation:
 Biodiversity Conservation Research Project, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Akihiro Kubo
Affiliation:
 Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Seiji Wakiyama
Affiliation:
 Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Yoriko Hatase
Affiliation:
 Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Masato Nagatsu
Affiliation:
 Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan

Abstract

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An investigation was carried out to monitor the escape and spread of oilseed rape (Brassica napus) transgenic plants and the introgression of transgenes to its closely related feral species in Japan. We screened a total of about 7500 feral B. napus, 300 B. rapa, and 5800 B. juncea seedlings from maternal plants in 143 locations at several ports, roadsides, and riverbanks. The presence of glufosinate-resistance or glyphosate-resistance transgenes in these seedlings was confirmed by means of herbicide treatments and also immunochemical and DNA analyses. B. napus plants with herbicide-resistant transgenic seeds were found at five of six major ports and along two of four sampled roadsides in the Kanto District. Transgenic oilseed rape plants have not been commercially cultivated in Japan, suggesting that the transgenes would probably have come from imported transgenic seeds that were spilled during transportation to oilseed processing facilities. No transgenes were detected in seeds collected from B. napus plants growing along riverbanks in the Kanto District or in seeds from closely related species (B. rapa and B. juncea). To our knowledge, this is the first published example of feral, transgenic populations occurring in a nation where the transgenic crop has not been cultivated commercially.

Keywords

Brassicaestablishmentglufosinateglyphosateherbicideintrogressiontransgenic plant
Type
Research Article
Information
Environmental Biosafety Research , Volume 4 , Issue 4 , October 2005 , pp. 217 - 222
Copyright
© ISBR, EDP Sciences, 2006

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