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Population size and incidence of virus infection in free-living populations of Cucurbita pepo

Published online by Cambridge University Press:  16 December 2008

Hector Quemada ,
Laura Strehlow ,
Deena S. Decker-Walters  and
Jack E. Staub
Hector Quemada
Affiliation:
Department of Biological Sciences, Wood Hall, Western Michigan University Kalamazoo, MI 49008, USA Current address: Department of Biology, Calvin College, 1726 Knollcrest Circle, S.E., Grand Rapids, MI 49546, USA
Laura Strehlow
Affiliation:
Department of Biological Sciences, Wood Hall, Western Michigan University Kalamazoo, MI 49008, USA
Deena S. Decker-Walters
Affiliation:
The Cucurbit Network, PO Box 560483, Miami, FL 33256, USA Current address: 5305 Mail Creek Lane, Fort Collins, CO 80525, USA
Jack E. Staub
Affiliation:
USDA/ARS, Vegetable Crops Research Unit, Department of Horticulture, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706, USA Current address: USDA, ARS, NPA, Forage and Range Research, Utah State University, 696 North 1100 East, Logan, UT 84322, USA

Abstract

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Impact assessments of virus resistance transgene introgression into wild, free-living populations are important for determining whether these transgenes present a risk to agriculture or the environment. Transgenic virus-resistant Cucurbita pepo ssp. ovifera var. ovifera L. (squash) cultivars have been commercialized, and may be cultivated in close proximity to cross-compatible wild, free-living relatives (C. pepo subsp. pepo vars. ozarkana and texana). Therefore, the potential impact of these virus resistance transgenes was studied by surveying the incidence and fluctuations of virus infection (as assayed by ELISA), virus symptoms (which may not be seen in an infected plant) and population size in forty-three free-living C. pepo populations in Illinois, Missouri, Arkansas, Mississippi, Louisiana, and Texas. Ten of these populations were studied over three consecutive seasons. Depending on the year, 61% to 78% percent of the populations had at least one individual infected by at CMV, ZYMV or WMV2, but the median incidence of infection within populations was 13%. The observed infection level in free-living populations was consistent with levels defined as “low” in field plot experiments conducted by others, leading to the conclusion that transgenic virus resistance should not provide a significant fitness advantage to the free-living populations examined. Viral symptoms were detected in only 2% of plants observed, indicating that severity of viral infection was low. CMV, ZYMV, and WMV2 were not the only viruses infecting these populations, further reducing the likelihood that resistance to these viruses would release populations from constraints imposed by virus diseases.

Keywords

transgenicvirus resistancepopulationsCucurbita pepointrogressiongene flow
Type
Research Article
Information
Environmental Biosafety Research , Volume 7 , Issue 4 , October 2008 , pp. 185 - 196
Copyright
© ISBR, EDP Sciences, 2008

References

Arias, DM, Rieseberg, LH (1994) Gene flow between cultivated and wild sunflower. Theor. Appl. Genet. 89: 655–660 CrossRef
Boyette, G, Templeton, E, Oliver, LR (1984) Texas gourd (Cucurbita texana) control. Weed Science 32: 649–655
Bridges DC (1992) Crop Losses Due to Weeds in Canada and the United States. Bridges DC, ed, Champaign, IL, Weed Science Society of America
Brookes, G, Barfoot, P (2005) GM crops: the global economic and environmental impact – the first nine years 1996–2004. AgBioForum 8: 187–196
Brookes, G, Barfoot, P (2006) Global impact of biotech crops: Socio-economic and environmental effects in the first ten years of commercial use. AgBioForum 9: 138–151
Burke, JM, Rieseberg, LH (2003) Fitness effects of transgenic disease resistance in sunflowers. Science 300: 1250 CrossRef
Chung, SM, Decker-Walters, DS, Staub, JE (2006) Cultivar-to-wild population introgression in Cucurbita pepo subsp. ovifera. J. New Seeds 8: 1–18 CrossRef
Decker, DS (1988) Origin(s), evolution, and systematics of Cucurbita pepo (Cucurbitaceae). Econ. Botany 42: 4–15 CrossRef
Decker-Walters, DS, Walters, TW, Cowan, CW, Smith, BD (1993) Isozymic characterization of wild populations of Cucurbita pepo. J. Ethnobiol. 13: 55–72
Decker-Walters, DS, Staub, JE, Chung, S-M, Nakata, E, Quemada, HD (2002) Diversity in free-living populations of Cucurbita pepo (Cucurbitaceae) as assessed by random amplified polymorphic DNA. Syst. Botany 27: 19–28
Frey, A, Di Canzio, J, Zurakowski, D (1998) A statistically defined endpoint titer determination method for immunoassays. J. Immunol. Methods 221: 35–41 CrossRef
Fuchs, M, Chirco, EM, Gonsalves, D (2004a) Movement of coat protein genes from a commercial virus-resistant transgenic squash into a wild relative. Environ. Biosafety Res. 3: 5–16 CrossRef
Fuchs, M, Chirco, EM, McFerson, J, Gonsalves, D (2004b) Comparative fitness of a wild squash species and three generations of hybrids between wild X virus-resistant transgenic squash. Environ. Biosafety Res. 3: 17–28 CrossRef
Hancock, J (2003) A framework for assessing the risk of transgenic crops. BioScience 53: 512–519 CrossRef
Kirkpatrick, KJ, Wilson, HD (1988) Interspecific gene flow in Cucurbita: C. texana vs. C. pepo. Am. J. Bot. 75: 519–527 CrossRef
Klas, F, Fuchs, M, Gonsalves, D (2006) Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash. Transgenic Res. 15: 527–541 CrossRef
Kuti, JO, Moline, HR (1986) Effects of inoculation with a mild strain of tomato aspermy virus on the growth and yield of tomatoes and the potential for cross protection. J. Phytopathol. 115: 56–60 CrossRef
Linder, CR, Taha, I, Seiler, G, Snow, A, Rieseberg, L (1998) Long-term introgression of crop genes into wild sunflower populations. Theor. Appl. Genet. 96: 339–347 CrossRef
Madden, LV, Hughes, G (1999) Sampling for plant disease incidence. Phytopathology 89: 1088–1103 CrossRef
Pallet DW, Milou IT, Edwards M, Naylor M, Wang H, Alexander M, Gray AJ, Mitchell E, Raybould A, Walsh JA, Cooper JI (2004) A tiered approach to risk assessment of virus resistance traits based on studies with wild Brassicas in England. In den Nijs HCM, Bartsch D, Sweet J, eds, Introgression from Genetically Modified Plants Into Wild Relatives, pp 309–322
Perring, TM, Farrar, CA, Blua, MJ, Wang, HL, Gonsalves, D (1995) Cross protection of cantaloupe with a mild strain of zucchini yellow mosaic virus: effectiveness and application. Crop Prot. 14: 601–606 CrossRef
Pilson D (2000) Herbivory and natural selection on flowering phenology in wild sunflower, Helianthus annuus. Oecologia 122: 72–82
Pilson, D, Decker, KL (2002) Compensation for herbivory in wild sunflower: response to simulated damage by the head-clipping weevil. Ecology 83: 3097–3107 CrossRef
Raybould A, Cooper I (2005) Tiered tests to assess the environmental risk of fitness changes in hybrids between transgenic crops and wild relatives: the example of virus resistant Brassica napus. Environ. Biosafety Res. 4: 127–140
Reagon, M, Snow, AA (2006) Cultivated Helianthus annuus (Asteraceae) as a genetic “bridge” to weedy sunflower populations in North America. Am. J. Bot. 93: 127–133 CrossRef
Rieseberg, LH, Kim, MJ, Seiler, GJ (1999) Introgression between cultivated sunflowers and a sympatric wild relative, Helianthus petiolaris (Asteraceae). Int. J. Plant Sci. 160: 102–108 CrossRef
Sankula S (2006) Quantification of the impacts on US agriculture of biotechnology-derived crops planted in 2005. National Center for Food and Agricultural Policy, Washington, D.C.
Sankula S, Blumenthal E (2004) Impacts on US agriculture of biotechnology-derived crops planted in 2003 – an update of eleven case studies. National Center for Food and Agricultural Policy, Washington, D.C.
Smith BD, Cowan CW, Hoffman MP (1992) Is it an indigene or a foreigner? In Smith BD, ed, Rivers of change: essays on the origins of agriculture in eastern North America. Smithsonian Institution Press, Washington, D.C., pp 67–100
Snow, AA, Moran-Palma, P, Rieseberg, LH, Wszelaki, A, Seiler, GJ (1998) Fecundity, phenology, and seed of F1 wild-crop hybrids in sunflower (Helianthus annuus, Asteraceae). Am. J. Bot. 85: 794–801 CrossRef
Snow, AA, Pilson, D, Rieseberg, LH, Paulsen, MJ, Pleskac, N, Reagon, MR, Wolf, DE, Selbo, SM (2003) A Bt Transgene reduces herbivory and enhances fecundity in wild sunflowers. Ecol. Appl. 13: 279–286 CrossRef
Spence, NJ, Mead, A, Miller, A, Shaw, ED, Walkey, DGA (1996) The effect on yield in courgette and marrow of the mild strain of zucchini yellow mosaic virus used for cross-protection. Ann. Appl. Biol. 129: 247–259 CrossRef
Spencer, LJ, Snow, AA (2001) Fecundity of transgenic wild-crop hybrids of Cucurbita pepo (Cucurbitaceae): implications for crop to wild gene flow. Heredity 86: 694–702 CrossRef
US National Research Council (2000) Genetically modified pest-protected plants: science and regulation. National Academy Press, Washington DC
US National Research Council (2002) Environmental effects of transgenic plants: the scope and adequacy of regulation. National Academy Press, Washington DC
USDA (1994a) Availalability of determination of nonregulated status for virus resistant squash. Federal Register 59: 64187–64189
USDA (1994b) APHIS/USDA Petition 92-204-01 for determination of nonregulated status for ZW-20 squash. Environmental Assessment and Finding of No Significant Impact. http://www.aphis. usda.gov/brs/aphisdocs2/92_20401p_com.pdf
USDA (1994c) USDA/APHIS Petition P93-106-01 for Determination That BXNTM Cotton Poses No Plant Pest Risk. Environmental Assessment February 1994. Finding of No Significant Impact (FONSI) for Nonregulatory Status of BXNTM Cotton, Calgene Petition P93-1096-01. http:// www.aphis.usda.gov/brs/aphisdocs2/93_19601p_com.pdf
USDA (1994d) Response to Calgene Petition 94-090-01p for Determination of Nonregulated Status for Laurate Canola Lines. Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/94_09001p_com.pdf
USDA (1995a) Monsanto Petition 95-045-01p to USDA/APHIS for Determination of Nonregulated Status of Glyphosate Tolerant Cotton (Roundup ReadyTM). Environmental Assessment and Finding of No Significant Impact. http://www.aphis.usda.gov/brs/aphisdocs2/ 95_04501p_com.pdf
USDA (1995b) USDA/APHIS Determination on a Petition 94-308-01p of Monsanto Agricultural Company Seeking Nonregulated Status of Lepidopteran-resistant Cotton Lines 531, 757, 1076. Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/94_30801p_com.pdf
USDA (1996a) Asgrow Seed Company: Availability of Determination of Nonregulated Status for Squash Line Genetically Engineered for Virus Resistance. Federal Register 61: 33484–33485
USDA (1996b) USDA/APHIS Petition 95-352-01p for Determination of Nonregulated Status for CZW-3 Squash. Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/95_35201p_com.pdf
USDA (1996c) Dupont Petition 95-256-01p to USDA/APHIS for Determination of Nonregulated Status of Sulfonylurea Tolerant Cotton Line 19-51a. Environmental Assessment and Finding of No Significant Impact. http://www.aphis.usda.gov/brs/aphisdocs2/95_25601p_com.pdf
USDA (1997a) USDA/APHIS Petition 97-013-01p for Determination of Nonregulated Status for Events 31807 and 31808 Cotton. Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/97_01301p_com.pdf
USDA (1997b) USDA/APHIS Petition 97-013-01p for Determination of Nonregulated Status for Events 310807 and 31808 Cotton. Evironmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/97_01301p_com.pdf
USDA (1998a) AgrEvo USA Company Petition 98-329-01p. Determination of Nonregulated Status for Glufosinate Tolerant Rice Transformation Events LLRICE06 and LLRICE62. Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/98_32901p_com.pdf
USDA (1998b) Response to AgrEvo Petition 97-205-01p for Determination of Nonregulated Status for Glufosinate Tolerant Canola. Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/97_20501p_com.pdf
USDA (1999a) Response to AgrEvo Petition 98-278-01p for Determination of Nonregulated Status for Canola Transformation Events MS8 and RF3 Genetically Engineered for Pollination Control and Tolerance to Glufosinate Herbicide. Finding of No Significant Impact.
USDA (1999b) Response to Monsanto Petition 98-216-01p for Determination of Nonregulated Status for Glyphosate-Tolerant Canola Line RT73. Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/98_21601p_com.pdf
USDA (2002a) Approval of Monsanto Company Petition (00-342-01p) Seeking a Determination of Nonregulated Status for Bollgard II Cotton Event 15985 Producing the Cry2Ab Insect Control Protein Derived from Bacillus thuringiensis subsp. Kurstaki. Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/00_34201p_com.pdf
USDA (2002b) USDA/APHIS Decision on Monsanto Company Request (01-324-01p) Seeking an Extension of Determination of Nonregulated Status for Glyphosate Tolerant Canola Event GT200. Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/01_32401p_com.pdf
USDA (2002c) Approval of Aventis CropScience USA LP (01-206-01p) Seeking Extension of Determination of Non-regulated Status for Male Sterile, Fertility Restoration, Glufosinate Tolerant Canola Transformation MS1 and RF1/RF2. Finding of No Significant Impact. http://www.aphis. usda.gov/brs/aphisdocs2/01_20601p_com.pdf
USDA (2002d) USDA/APHIS Decision on Aventis CropScience USA LP Request (01-206-02p) Seeking and Extension of Determination of Nonregulated Status for Glufosinate Tolerant Canola Event Topas 19/2. Finding of No Significant Impact. http://www.aphis.usda.gov/brs/aphisdocs2/ 01_20602p_com.pdf
USDA (2003) Approval of Aventis CropScience USA LP Petition (02-042-01p) Seeking a Determination of Non-regulated Status for Glufosinate-ammonium Herbicide-tolerant Cotton Transformation Event LLCotton25. http:// www.aphis.usda.gov/brs/aphisdocs2/02_04201p_com.pdf
USDA (2004a) Approval of Monsanto Company Request (04-086-01p) Seeking a Determination of Non-regulated Status for Glyphosate Tolerant Cotton Event MON 88913. http://www.aphis. usda.gov/brs/aphisdocs2/04_08601p_com.pdf
USDA (2004b) Approval of Mycogen/Dow Petitions 03-036-01p and 03-036-02p Seeking Determination of Nonregulated Status for Insect-Resistant Cotton Events 281-24-236 and 3006-210-23 Genetically Engineered to Express Synthetic B.t. Cry1F and Cry1Ac, Respectively. Environmental Assessment and Finding of No Significant Impact. http://www.aphis.usda.gov/ brs/aphisdocs2/03_03601p_com.pdf
USDA (2005) Syngenta Petition 03-155-01p for Determination of Nonregulated Status for Lepidopteran Resistant Cotton Event COT102. USDA/APHIS Environmental Assessment and Finding of No Significant Impact. http:// www.aphis.usda.gov/brs/aphisdocs2/03_15501p_com.pdf
USDA (2006) Finding of No Significant Impact. Animal Plant Health Inspection Service. Extension of Nonregulated Status to Rice Line LLRICE601. http://www.aphis.usda.gov/brs/ aphisdocs2/06_23401p_com.pdf.
Whitton, JD, Wolf, E, Arias, DM, Snow, AA, Rieseberg, LL (1997) The persistence of cultivar alleles in wild populations of sunflowers five generations after hybridization. Theor. Appl. Genet. 95: 33–40 CrossRef
Wilson, HD (1990) Gene flow in squash species. BioScience 40: 449–455 CrossRef