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The 6-hour soluble antigen production test for comparing the infectivity of influenza virus preparations

Published online by Cambridge University Press:  15 May 2009

N. B. Finter  and
A. J. Beale
N. B. Finter
Affiliation:
Public Health Laboratory, Northampton
A. J. Beale
Affiliation:
Public Health Laboratory, Northampton
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1. The 6 hr. soluble antigen production test for comparing the infectivity of influenza virus preparations is described in detail. Dilutions of the preparations are inoculated intra-allantoically into groups of fertile eggs, and after 6 hr. of incubation the amounts of soluble antigen formed in their chorio-allantoic membranes are determined. Reproducible results can be obtained.

2. The similar test, in which haemagglutinins in the chorio-allantoic membranes are measured 6 hr. after inoculation of seed virus, is less accurate and less sensitive.

3. Eggs of different ages of incubation form different amounts of soluble antigen in their chorio-allantoic membranes in 6 hr. when inoculated with the same amounts of virus.

Type
Research Article
Information
Journal of Hygiene , Volume 54 , Issue 1 , March 1956 , pp. 58 - 67
Copyright
Copyright © Cambridge University Press 1956

References

REFERENCES

Beale, A. J. (1954). A comparison of the soluble antigen production by tissues infected with preparations of extracellular and intracellular influenza virus. J. Hyg., Camb., 52, 225.CrossRefGoogle ScholarPubMed
Beale, A. J. & Finter, N. B. (1956). The infectivity of chorio-allantoic membrane influenza virus and incomplete influenza virus by the 6 hour soluble antigen production test. J. Hyg., Camb., 53, 68.CrossRefGoogle Scholar
Edney, M. & Isaacs, A. (1950). Interference between inactive and active influenza viruses in the chick embryo. 3. Inhibitor of virus haemagglutination in the chorio-allantoic membrane. Aust. J. exp. Biol. 28, 603.CrossRefGoogle Scholar
Finter, N. B., Liu, O. C., Lieberman, L. & Henle, W. (1954). Studies on host-virus interactions in the chick embryo-influenza virus system. 8. An experimental analysis of various de-embryonation technics. J. exp. Med. 100, 33.CrossRefGoogle Scholar
Hoyle, L. (1945). An analysis of the complement-fixation test in influenza. J. Hyg., Camb., 44, 170.CrossRefGoogle Scholar
Hoyle, L. (1948 a). The growth cycle of influenza virus A. A study of the relations between virus, soluble antigen and host cell in fertile eggs inoculated with influenza virus. Brit. J. exp. Path. 29, 390.Google ScholarPubMed
Hoyle, L. (1948 b). Technique of the complement fixation test in influenza. Mon. Bull. Minist. Hlth Lab. Serv. 7, 114.Google ScholarPubMed
Hoyle, L. (1952). The multiplication of complement-fixing antigen and red-cell agglutinin in the chorio-allantoic membrane of fertile eggs inoculated with influenza virus. J. Path. Bact. 64, 419.CrossRefGoogle ScholarPubMed
Knight, C. A. (1944). Titration of influenza virus in chick embryos. J. exp. Med. 79, 487.CrossRefGoogle ScholarPubMed
von Magnus, P. (1951). Propagation of the PR8 strain of influenza A virus in chick embryos. 1. The influence of various experimental conditions on virus multiplication. Acta path. microbiol. scand. 28, 250.CrossRefGoogle Scholar