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Allelic variation in the Helicobacter pylori flagellin genes flaA and flaB: its consequences for strain typing schemes and population structure

Published online by Cambridge University Press:  15 May 2009

K. J. Forbes ,
Z. Fang  and
T. H. Pennington
K. J. Forbes
Affiliation:
Medical Microbiology, Aberdeen University, Forresterhill, Aberdeen AB9 2ZD
Z. Fang
Affiliation:
Medical Microbiology, Aberdeen University, Forresterhill, Aberdeen AB9 2ZD
T. H. Pennington
Affiliation:
Medical Microbiology, Aberdeen University, Forresterhill, Aberdeen AB9 2ZD
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Extensive DNA sequence diversity was noted in Helicobacter pylori flagellin genes flaA and flaB. PCR amplified sequences from 49 isolates were digested with AluI. HindIII. MboI or MspI, the resultant patterns were compared between the different isolates and these used to differentiate the isolates from each other. Evidence that the extensive diversity that was found in these genes is the result of reassortment of sequences between strains in the bacterial population is presented, such that a comparatively small number of individual sequence mutations can recombine together in random combinations to form a greater number of distinct alleles. Geographical differences in the predominant patterns in the flaA alleles were also observed and could reflect regional differences either in the human host population or in the bacterial population. In view of the genetic complexity of this species, molecular typing schemes designed to identify related strains may falsely associate strains if the methods do not characterize sufficient genetic sites to exclude chance associations of genetic markers in strains which are actually not closely related to each other.

Type
Research Article
Information
Epidemiology & Infection , Volume 114 , Issue 2 , April 1995 , pp. 257 - 266
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Marshall, BJ.Warren, JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984; i: 1311–15.CrossRefGoogle ScholarPubMed
2.Forman, D, Newell, DG, Fullerton, F et al. , Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. BMJ 1991; 302: 1302–5.CrossRefGoogle ScholarPubMed
3.Banatvala, N, Feldman, R. The epidemiology of Helicobacter pylori: missing pieces in the jigsaw. Commun Dis Rep; 3: R56–9.Google Scholar
4.Taylor, DE, Eaton, M, Chang, M, Salam, SM. Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level. J Bacteriol 1992; 174: 6800–6.CrossRefGoogle ScholarPubMed
5.Majewski, SIH, Goodwin, CS. Restriction endonuclease analysis of the genome of Helicobacter pylori with a rapid extraction method: evidence for considerable genomic variation. J Infect Dis 1988: 157: 465–71.Google Scholar
6.Akopyanz, N, Bukanov, NO, Westblom, TU, Kresovich, S, Berg, DE. DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucl Acids Res 1992; 20: 5137–42.CrossRefGoogle ScholarPubMed
7.Akopyanz, N, Bukanov, NO, Westblom, TU, Berg, DE. PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori. Nucl Acids Res 1992; 20: 6221–5.CrossRefGoogle ScholarPubMed
8.Desai, M, Linton, D, Owen, RJ, Stanley, J. Molecular typing of Helicobacter pylori isolates from asymptomatic, ulcer and gastritis patients by urease polymorphism. Epidemiol Infect 1994; 112: 151–60.CrossRefGoogle ScholarPubMed
9.Eaton, KA, Morgan, DR, Krakowla, SA. Helicobacter pylori virulence factors in gnotobiotic piglets. Infect Immun 1989; 57: 1119–25.CrossRefGoogle Scholar
10.Leying, H, Suerbaum, S, Geis, G, Haas, R. Cloning and characterisation of a Helicobacter pylori flagellin gene. Mol Microbiol 1992; 6: 2863–74.CrossRefGoogle ScholarPubMed
11.Suerbaum, S, Josenhans, C, Labigne, A. Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA- and flaB-negative mutants by electroporation-mediated allelic exchange. J Bacteriol; 1993; 175: 3278–88.CrossRefGoogle ScholarPubMed
12.Nwokolo, CU.Bickley, J, Attard, AR, Owen, RJ, Costas, M, Fraser, IA. Evidence of clonal variants of Helicobacter pylori in three generations of a duodenal ulcer disease family. Gut 1992; 33: 1323–7.CrossRefGoogle ScholarPubMed
13.Owen, RJ, Fraser, J, Costas, M, Morgan, D, Morgan, DR. Signature patterns of DNA restriction fragments of Helicobacter pylori before and after treatment. J Clin Pathol 1990; 43: 646–9.CrossRefGoogle ScholarPubMed
14.Lagenberg, W, Rauws, EAJ, Widjojokusumo, A, Tytgat, GNJ, Zanen, HC. Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J Clin Microbiol 1986; 24: 414–7.CrossRefGoogle Scholar
15.Moore, RA, Kureishi, A, Wong, S, Bryan, LE. Categorization of clinical isolates of Helicobacter pylori on the basis of restriction digest analyses of PCR-amplified ureC genes. J Clin Microbiol 1993: 31: 1334–5.CrossRefGoogle Scholar
16.Selander, RK, Caugant, DA, Whittam, TS. Genetic structure and variation in natural populations of Escherichia coli. In: Neiderhardt, FC, Ingraham, JL, Low, KB, Magasanik, B, Schaechter, M, Umbarger, HE, eds. Escherichia coli and Salmonella typhimurium cellular and molecular biology. Washington, DC: Am Soc Microbiol, XX: 1625–48.Google Scholar
17.Musser, JM, Kroll, JS, Granoff, DM et al. , Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis 1990; 12: 75–111.CrossRefGoogle ScholarPubMed
18.Nedenskov-Sorensen, P, Bukholm, G, Bovre, K. Natural competence for genetic transformation in Campylobacter pyloridis. J Infect Dis 1990; 161: 365–6.CrossRefGoogle Scholar
19.Wang, Y, Roos, KP, Taylor, DE. Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenieal resistance marker. J Gen Microbiol 1993; 139: 2485–93.CrossRefGoogle ScholarPubMed
20.O'Rourke, M, Stevens, E. Genetic structure of Neisseria gonorrhoeae populations: a non-clonal pathogen. J General Microbiol 1993; 139: 2603–11.CrossRefGoogle ScholarPubMed
21.Forbes, KJ, Bruce, KD, Ball, A, Pennington, TH. Variation in length and sequence of porin (ompP2) alleles of non-capsulate Haemophilus influenzae. Mol Microbiol 1992; 6: 2107–12.CrossRefGoogle ScholarPubMed
22.O'Rourke, M, Spratt, BG. Further evidence for the non-clonal population structure of Neisseria gonorrhoeae: extensive genetic diversity within isolates of the same electrophoretic type. Microbiol 1994; 140: 1285–90.CrossRefGoogle ScholarPubMed