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An evaluation of a commercially available enzyme immunoassay test for the rapid detection of salmonellae in food and environmental samples

Published online by Cambridge University Press:  19 October 2009

J. P. Harford
J. P. Harford
Affiliation:
Public Health Laboratory, Bridle Path, York Road, Leeds
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Summary

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A total of 91 food and environmental samples were examined for the presence of salmonollao using a commercially available enzyme immunoassay kit (EIA) and a conventional culture technique. A 78% agreement was obtained, but re-examination of culture-negative, EIA-positive samples gave agreement of 86%. The problem of comparing EIA and culture results is discussed. A partially selective pre-enrichment broth was tested in 37 samples and gave better EIA ratios. Artificially contaminated cooked foods gave 100% agreement.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 1 , August 1987 , pp. 127 - 136
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Beckers, H. J., Van Leusden, F. M., Meijssen, M. J. M. & Kamplemacher, E. H. (1985). Reference material for the evaluation of a standard method for the detection of salmonellas in foods and feeding stuffs. Journal of Applied Bacteriology 59, 223–230.CrossRefGoogle ScholarPubMed
D'Aoust, J. Y. (1984). Salmonella detection in foods: present status and research needs for the future. Journal of Food protection 47, 78–81.CrossRefGoogle ScholarPubMed
Flowers, R. S. (1985). Comparison of rapid salmonella screening methods and the conventional culture method. Food Technology 39, 103–108.Google Scholar
Harvey, R. W. S. & Price, T. H. (1974). Isolation of Salmonellas. Public Health Laboratory Service Monograph Series No. 8. London: Her Majesty's Stationery Office.Google Scholar
Harvey, R. W. S. & Price, T. H. (1979). Principles of salmonella isolation. Journal of Applied Bacteriology 46, 27–56.CrossRefGoogle ScholarPubMed
Krysinski, E. P. & Heimsch, R. C. (1977). Use of enzyme labelled antibodies to detect salmonella in foods. Applied and Environmental Microbiology 33, 947–954.CrossRefGoogle ScholarPubMed
Mattingly, J. A., Robison, B. J., Boehm, A. & Gehle, W. D. (1985). Use of monoclonal antibodies for the detection of salmonellae in foods. Food Technology 39, 90–94.Google Scholar
Minnich, S. A., Hartman, P. A. & Heimsch, R. C. (1982). Enzyme immunoassay for the detection of salmonellae in foods. Applied and Environmental Microbiology 43, 877–883.CrossRefGoogle ScholarPubMed
Mohr, H. K., Trenk, H. L. & Yeterian, M. (1974). Comparison of fluorescent-antibody methods and enrichment serology for the detection of salmonella. Applied and Environmental Microbiology 27, 234.Google ScholarPubMed
Robison, B. J., Pretzman, C. I. & Mattingly, J. A. (1983). Enzyme immunoassay in which a myeloma protein is used for detection of salmonellae. Applied and Environmental Microbiology 45, 1816–1821.CrossRefGoogle ScholarPubMed
Rolfe, V. (1946). A note on the preparation of tetrathionate broth. Monthly Bulletin of the Ministry of Health Laboratory Service 5, 158.Google ScholarPubMed
Smith, A. M. & Jones, C. (1983). Use of Murine Myeloma Protein M467 for detecting Salmonella spp. in milk. Applied and Environmental Microbiology 46, 826–831.CrossRefGoogle ScholarPubMed
Sperber, W. H. & Deibel, R. H. (1969). Accelerated procedure for salmonellae detection in dried foods and feeds involving only broth cultures and serological reagents. Applied Microbiology 17, 533–539.CrossRefGoogle Scholar
Van Leusden, F. M., Van Schothorst, M. & Beckers, J. J. (1982). The standard salmonella isolation method. In Isolation and Identification Methods for Food Poisoning Organisms, Society for Applied Bacteriology, Technical Series No. 17 (ed. Corry, J. E. L., Roberts, D. and Skinner, F. A.), pp. 35–49. London: Academic Press.Google Scholar
Van Schothorst, M. & Renaud, A. M. (1983). Dynamics of salmonella isolation with modified Rappaport's medium (R10). Journal of Applied Bacteriology 54, 209–215.CrossRefGoogle ScholarPubMed
Van Schothorst, M. & Renaud, A.M. (1985). Malachite green pre-enrichment medium for improved salmonellae isolation from heavily contaminated samples. Journal of Applied Bacteriology 59, 223–230.CrossRefGoogle ScholarPubMed