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Antibiotic sensitivity and mutation rates to antibiotic resistance in Mycoplasma mycoides ssp. mycoides

Published online by Cambridge University Press:  19 October 2009

D. H. Lee ,
R. J. Miles  and
J. R. M. Inal
D. H. Lee
Affiliation:
Department of Microbiology, Kings College, University of London, Campden Hill Road, London WS 7AH
R. J. Miles
Affiliation:
Department of Microbiology, Kings College, University of London, Campden Hill Road, London WS 7AH
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The antibiotic resistance of Mycoplasma mycoides ssp. mycoides strain T1 was investigated. This strain was resistant to high levels ( > 100 μg ml−1) of rifampicin and nalidixic acid. It was sensitive to streptomycin, spectinomycin and novobiocin; however, single step mutants with high levels of resistance ( > 100 μg ml−1) were readily isolated. With erythromycin and tylosin for which the minimum inhibitory concentration (MIC) for the parent strain was < 0·1 μg ml−1, mutants resistant to > 100 μg ml−1 were obtained in two and three steps respectively. The MIC of tetracycline in single step resistant mutants (0·6 μg ml−1) was tenfold higher than the parent strain, but could not be increased further. There was only a twofold increase in resistance to chloramphenicol in single step mutants. The frequency of resistant mutants varied with the antibiotic and was between 4× 10minuss;6 and 2× 10−8. The mutation rate to antibiotic resistance to streptomycin, spectinomycin, novobiocin, erythromycin and tylosin was between 3× 10−8 and 5× 10−9 per cell per generation. There was a fivefold decrease in mutation rate to resistance to 60 μg ml−1 streptomycin compared to that to 20 μg ml−1.

Type
Research Article
Information
Epidemiology & Infection , Volume 98 , Issue 3 , June 1987 , pp. 361 - 368
Copyright
Copyright © Cambridge University Press 1987

References

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