Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-28T16:34:45.406Z Has data issue: false hasContentIssue false
  • English
  • Français

Antibody against viruses in maternal and cord sera: non-specific inhibitors are found to higher titre on the maternal side of the circulation

Published online by Cambridge University Press:  19 October 2009

P. D. Griffiths ,
D. Girdhar ,
S. Fisher-Hoch ,
M. W. Race  and
R. B. Heath
P. D. Griffiths
Affiliation:
Virology Department, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE
D. Girdhar
Affiliation:
Virology Department, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE
S. Fisher-Hoch
Affiliation:
Virology Department, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE Special Pathogens Reference Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, SP4 0JG
M. W. Race
Affiliation:
Virology Department, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE Special Pathogens Reference Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, SP4 0JG
R. B. Heath
Affiliation:
Virology Department, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Pregnancies were identified in which maternal IgG antibodies against rubella virus were not detectable by single radial haemolysis. Twenty paired maternal/cord sera were then tested for haemagglutination-inhibiting (HI) activity against rubella virus without kaolin pretreatment of the sera. In the absence of specific antibody, the HI activity observed could thus be ascribed to the effect of non-specific inhibitors. The HI activity in maternal sera was significantly (P < 0·001) higher than that in cord sera. The 20 pairs of sera were similarly tested against a bunyavirus, an alphavirus and a flavivirus, both with and without kaolin pretreatment. The results showed non-specific inhibitors were found to higher titre in maternal sera, with the difference being statistically significant (P < 0·001) for each of the three viruses.

Type
Research Article
Information
Journal of Hygiene , Volume 92 , Issue 3 , June 1984 , pp. 297 - 301
Copyright
Copyright © Cambridge University Press 1984

References

Armitage, P. (1971). Statistical Methods in Medical Research. Oxford: Blackwell.Google Scholar
Brouwer, R., De Groot, I. G. M. & Verheij, F. B. M. (1974). Comparison of maternal and cord serum titres for measles and for rubella antibodies. Archiv für die gesamte Virusforschung 44, 237.CrossRefGoogle ScholarPubMed
Clarke, D. H. & Casals, J. (1958). Techniques for haemagglutination-inhibition with arthropodborne viruses. American Journal of Tropical Medicine and Hygiene 7, 561.CrossRefGoogle Scholar
Doherty, N., Ronalds, C. J., Pattison, J. R. & Heath, R. B. (1975). A comparative study of methods used for removing non-specific inhibitors of rubella haemagglutination from human sera. Medical Laboratory Technology 32, 317.Google ScholarPubMed
Griffiths, P. D., Ronalds, C. J. & Heath, R. B. (1980). A prospective study of influenza infections during pregnancy. Journal of Epidemiology and Community Health 34, 124.CrossRefGoogle ScholarPubMed
Griffiths, P. D., Berney, S. I., Argent, S. & Heath, R. B. (1982). Antibody against viruses in maternal and cord sera: specific antibody is concentrated on the fetal side of the circulation. Journal of Hygiene 89, 303.CrossRefGoogle ScholarPubMed
Griffiths, P. D. & Baboonian, C. (1984). A prospective study of primary cytomegalovirus infection during pregnancy (final report). British Journal of Obstetrics and Gynaecology (in press).CrossRefGoogle ScholarPubMed
Grint, P. C. A., Argent, S. & Heath, R. B. (1982). The postnatal acquisition of factors which affect the influenza haemagglutination-inhibition test. Journal of Hygiene 89, 329.CrossRefGoogle ScholarPubMed
Kohler, P. F. & Farr, R. S. (1966). Elevation of cord over maternal IgG immunoglobulin: evidence for an active placental IgG transport. Nature 210, 1070.CrossRefGoogle ScholarPubMed
Longsworth, L. G., Curtis, R. M. & Pembroke, R. H. (1945). The electrophoretic analysis of maternal and fetal plasmas and sera. Journal of Clinical Investigation 24, 46.CrossRefGoogle ScholarPubMed
Masurel, N., De Bruijne, J. I., Beuningh, H. A. & Schouten, H. J. A. (1978). Haemagglutination-inhibition antibodies against influenza A and influenza B in maternal and neonatal sera. Journal of Hygiene 80, 13.CrossRefGoogle ScholarPubMed
Morgan-Capner, P., Pullen, H. J. M., Pattison, J. R., Bidwell, D. E., Bartlett, A. & Voller, A. (1979). A comparison of three tests for rubella antibody screening. Journal of Clinical Pathology 32, 542.CrossRefGoogle ScholarPubMed
Sarateanu, D. E., Ehrengut, W. & Fofana, Y. (1980). Influenza and corona-virus antibodies in the newborns and their mothers in Mali. Tropenmedizin und Parasitologie 31, 399.Google ScholarPubMed
Ventura, A. K., Ehrenkranz, N. J. & Rosenthal, D. (1975). Placental passage of antibodies to dengue virus in persons living in a region of hyperendemic dengue virus infection. Journal of Infectious Diseases 131, S 62.CrossRefGoogle Scholar