Hostname: page-component-745bb68f8f-b6zl4 Total loading time: 0 Render date: 2025-01-26T19:00:35.390Z Has data issue: false hasContentIssue false
  • English
  • Français

Antibody responses in patients with farmer's lung disease to antigens from Micropolyspora faeni

Published online by Cambridge University Press:  15 May 2009

M. R. Hollingdale
M. R. Hollingdale
Affiliation:
Mycological Reference Laboratory, London School of Hygiene and Tropical Medicine, London WGlE 7HT
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Three purified cell-wall glycoprotein antigens, a, b and c, sensitive to sodium periodate and resistant to pronase, were extracted from mycelium of Micropolyspora faeni with aqueous phenol or trichloracetic acid. Pronase-sensitive, sodium periodate-resistant protein antigens were extracted from mycelium with aqueous phenol. Immunoelectrophoresis was a critical method of assessing purity of preparation.

Antibodies to glycoprotein antigens only occurred in clinically defined cases of farmer's lung disease, whereas antibodies to protein antigens also occurred in symptomless farmers. Precipitins to a occurred in all cases of farmer's lung disease, and this antigen was isolated and purified.

Fractionation of pooled sera from cases of farmer's lung disease showed that precipitins were IgG globulins, whereas latex-agglutinating antibodies were IgG, IgA and especially IgM globulins. It is suggested that farmer's lung may involve a cytotoxic type II reaction, in which glycoproteins adsorbed to tissue cells react with IgG, IgA and IgM immunoglobulins in the presence of complement, causing cellular damage.

Type
Research Article
Information
Journal of Hygiene , Volume 72 , Issue 1 , February 1974 , pp. 79 - 89
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Barbee, R. A., Dickie, H. A. & Rankin, J. (1965). Pathogenicity of specific glycopeptide antigen in Farmer's Lung. Proceedings of the Society for Experimental Biology and Medicine 118, 546.CrossRefGoogle ScholarPubMed
Coombs, R. R. A. & Gell, P. G. H. (1968). Clinical Aspects of Immunology (ed. Gell, P. G. H. and Coombs, R. R. A.), p. 27. Oxford: Blackwell.Google Scholar
Fletcher, S. M., Rondle, C. J. M. & Murray, I. G. (1970). The extracellular antigens of Micropolyspora faeni: their significance in farmer's lung disease. Journal of Hygiene 68, 41.CrossRefGoogle ScholarPubMed
Fletcher, S. M. (1971). Antigens of Micropolyspora faeni and their relevance in farmer's lung disease. Ph.D. Thesis, University of London.Google Scholar
Fletcher, S. M. & Rondle, C. J. M. (1973). Micropolyspora faeni and farmer's lung disease. Journal of Hygiene 71, 185.CrossRefGoogle ScholarPubMed
Gierer, A. & Schram, G. (1956). Die Infektiosität der Nucleinsaure aus Tabakmosaikvirus. Zeitschrift für Naturforschung 11b, 138.CrossRefGoogle Scholar
Kobayashi, M., Stahmann, M. A., Rankin, J. & Dickie, H. A. (1963). Antigens in mouldy hay as the cause of Farmer's Lung. Proceedings of the Society for Experimental Biology and Medicine 113, 427.Google Scholar
LaBerge, D. E. & Stahmann, M. A. (1966 a). Antigens from mouldy hay involved in Farmer's Lung. Proceedings of the Society for Experimental Biology and Medicine 121, 458.CrossRefGoogle ScholarPubMed
LaBerge, D. E. & Stahmann, M. A. (1966 b). Antigens from Thermopolyspora polyspora involved in Farmer's Lung. Proceedings of the Society for Experimental Biology and Medicine 121, 463.CrossRefGoogle ScholarPubMed
Pepys, J. & Jenkins, P. A. (1965). Precipitin (f.l.h.) test in Farmer's Lung. Thorax 20, 21.CrossRefGoogle ScholarPubMed
Pepys, J., Jenkins, P. A., Festenstein, G. N., Gregory, P. H., Lacey, M. E. & Skinner, F. A. (1963). Farmer's Lung. Thermophilic actinomycetes as a source of ‘;farmer's lung hay’ antigens. Lancet ii, 607.CrossRefGoogle Scholar
Pepys, J. (1969). Hypersensitivity diseases of the lungs due to fungi and organic dusts. Monographs in Allergy, no. 4. Basel: Karger.Google Scholar
Pike, R. M. (1967). Antibody heterogeneity and serological reactions. Bacteriological Reviews 31, 157.CrossRefGoogle ScholarPubMed
Strannegård, O. & Yurchision, A. (1969). Formation of agglutinating and reaginic antibodies in rabbits following oral administration of soluble and particulate antigens. International Archives of Allergy 35, 579.CrossRefGoogle ScholarPubMed
Wenzel, F. J., Emanuel, D. A. & Gray, B. S. (1971). Immunofluorescent studies in patients with Farmer's Lung. Journal of Allergy and Clinical Immunology 48, 224.CrossRefGoogle ScholarPubMed
Westphal, O., Luderitz, O. & Bister, F. (1952). Über die Extraction von Bakterien mit Phenol/Wasser. Zeitschrift für Naturforschung 7b, 148.CrossRefGoogle Scholar