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Cell surface hydrophobicity, adherence to HeLa cell cultures and haemagglutination pattern of pyelonephritogenic Escherichia coli strains

Published online by Cambridge University Press:  15 May 2009

A. Brauner ,
M. Katouli ,
K. Tullus  and
S. H. Jacobson
A. Brauner*
Affiliation:
Department of Clinical Microbiology, Stockholm County Council and Karolinska Hospital, Stockholm, Sweden
M. Katouli
Affiliation:
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden
K. Tullus
Affiliation:
Department of Paediatrics, Danderyd Hospital, Stockholm, Sweden
S. H. Jacobson
Affiliation:
Department of Medicine, Division of Nephrology, Karolinska Hospital, Stockholm, Sweden
*
*Dr A. Brauner, Department of Clinical Microbiology, Stockholm County Council, PO Box 70470, S-107 26 Stockholm, Sweden.
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Cell surface hydrophobicity, haemagglutination pattern and adherence to HeLa cells were examined in 230 strains of Escherichia coli collected from women (n = 61 strains) and children (n = 65 strains) with non-obstructive acute pyelonephritis and in 104 faecal control strains of E. coli from healthy adults (n = 71 strains) and children (n = 33 strains). Pyelonephritogenic E. coli strains showed a significantly increased incidence of hydrophobic properties (90%) and mannose resistant haemagglutination (MRHA) of human erythrocytes (83%) than faecal control strains (64 and 23% respectively, P < 0·001 in both cases). Mannose sensitive haemagglutination (MSHA) was observed in 48% of the pyelonephritogenic E. coli strains and in 50% of the faecal control strains (NS). The incidence of adherence to HeLa cells was low both in pyelonephritogenic and faecal control strains, 6 and 7% respectively (NS). The bacterial phenotypes MRHA + MSHA + and MRHA + MSHA− appeared significantly more often in pyelonephritogenic E. coli strains (35 and 48% respectively) than in faecal control strains (5 and 17% respectively, P < 0·001 in both cases). The phenotype MRHA − MSHA + occurred significantly more often in control strains (45%) than in pyelonephritogenic strains (13%, P < 0·001). Eighty-three per cent of the pyelonephritogenic E. coli strains expressing hydrophobic properties showed MRHA and 50% of the hydrophobic strains showed MSHA. There were no significant correlations between cell surface hydrophobic properties and haemagglutination pattern or adherence to HeLa cells in pyelonephritogenic E. coli strains nor in faecal control strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 2 , October 1990 , pp. 255 - 263
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Duguid, JP, Clegg, S, Wilson, MI. The fimbrial and non-fimbrial haemagglutinins of Escherichia coli. J Med Microbiol 1979; 12: 213–27.CrossRefGoogle ScholarPubMed
2.Ofek, I, Beachey, EH. Mannose binding and epithelial cell adherence of Escherichia coli. Infect Immun 1978; 22: 247–54.CrossRefGoogle ScholarPubMed
3.Källenius, G, Svenson, SB, Möllby, R, Cedergren, B, Hultberg, H, Winberg, J. Structure of carbohydrate part of receptor on human uroepithelial cells or pyelonephritogenic Escherichia coli. Lancet 1981; ii: 604–6.CrossRefGoogle Scholar
4.Källenius, G, Svenson, SB, Hultberg, H, Möllby, R, Hellin, I, Cedergren, B, Winberg, J. Occurrence of P-fimbriated Escherichia coli. in urinary tract infections. Lancet 1981; ii: 1369–72.CrossRefGoogle Scholar
5.Jacobson, SH, Kallenius, G, Lins, L-E, Svenson, SB. P-fimbriated Escherichia coli in adults with acute pyelonephritis. J Infect Dis 1985; 152: 426–7.CrossRefGoogle Scholar
6.O'Hanley, P, Lark, D, Falkow, S, Schoolnik, G. Molecular basis of Escherichia coli colonization of the upper urinary tract in BALB/c mice. J Clin Invest 1985; 75: 347–60.CrossRefGoogle ScholarPubMed
7.Arthur, M, Johnson, CE, Rubin, RH et al. , Molecular epidemiology of adhesin and hemolysin virulence factors among uropathogenic Escherichia coli. Infect Immun 1989; 57: 303–13.CrossRefGoogle ScholarPubMed
8.Harber, MJ, Mackenzie, R, Chick, S, Asscher, AW. Lack of adherence to epithelial cells by freshly isolated urinary pathogens. Lancet 1982; i: 586–8.CrossRefGoogle Scholar
9.Lichodziejewska, M, Topley, M, Steadman, R, Mackenzie, RK, Verrier, Jones K, Williams, JD. Variable expression of P-fimbriae in Escherichia coli urinary tract infection. Lancet 1989; ii: 1414–18.CrossRefGoogle Scholar
10.Ivahi, T, Abe, Y, Nakao, M, Imada, A, Tsuchiya, K. Role of type 1 fimbriae in the pathogenesis of ascending urinary tract infection induced by Escherichia coli in mice. Infect Immun 1983; 39: 1307–15.CrossRefGoogle Scholar
11.Schaeffer, AJ, Schwan, WR, Hultgren, SC, Duncan, JL. Relationship of type 1 pilus expression in Escherichia coli to ascending urinary tract infections in mice. Infect Immun 1987; 55: 373–80.CrossRefGoogle ScholarPubMed
12.Öhman, L, Normann, B, Stendahl, O. Physiochemical surface properties of Escherichia coli strains isolated from different types of urinary tract infections. Infect Immun 1981; 32: 951–5.CrossRefGoogle Scholar
13.Sherman, PM, Houston, WL, Boedeker, EC. Functional heterogeneity of intestinal adherence to intestinal membranes and surface hydrophobicity. Infect Immun 1985; 49: 797–804.CrossRefGoogle ScholarPubMed
14.Jacobson, SH, Tullus, K, Brauner, A. Hydrophobic properties of Escherichia coli causing acute pyelonephritis. J Infect 1989; 19: 17–23.CrossRefGoogle ScholarPubMed
15.Scaletsky, ICA, Silva, MLM, Trabulsi, LR. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 1984; 45: 534–6.CrossRefGoogle ScholarPubMed
16.Carlsson, B, Gothefors, L, Ahlstedt, S, Hanson, LÅ, Winberg, J. Studies of Escherichia coli O antigen specific antibodies in human milk, maternal serum and cordal blood. Acta Paediatr Scand 1976; 65: 216–24.CrossRefGoogle Scholar
17.Evans, DG, Evans, DJ, Tjoa, W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolates from adults with diarrhoea. Correlation with colonization factor. Infect Immun 1977; 18: 330–7.CrossRefGoogle Scholar
18.Ljungh, Å, Wadström, T. Salt aggregation test for measuring cell surface hydrophobicity of urinary Escherichia coli. Eur J Clin Microbiol 1982; 1: 388–93.CrossRefGoogle ScholarPubMed
19.Källenius, G, Möllby, R. Adhesion of Escherichia coli to human periuretheral cells correlated to mannose-resistant agglutination of human erythrocytes. FEMS Microbiol Lett 1979; 5: 295–9.CrossRefGoogle Scholar
20.Svanborg-Edén, C, Eriksson, B, Hanson, LÅ, Jodal, U, Kaijser, B, Lidin-Janson, G, Lindberg, U, Oiling, S. Adhesion to normal human uroepithelial cells of Escherichia coli from children with various forms of urinary tract infection. J Pediatr 1978; 398–403.CrossRefGoogle Scholar
21.Hopkins, WJ, Jensen, JL, Uehling, DT, Balish, E. In vitro and in vivo adherance of uropathogenic Escherichia coli strains. J Urol 1986; 135: 1319–21.CrossRefGoogle ScholarPubMed
22.Opal, SM, Cross, A, Gemski, P, Lyhte, LW. Survey of purported virulence factors of Escherichia coli isolated from blood, urine and stool. Eur J Clin Microbiol Infect Dis 1988; 7:425–7.CrossRefGoogle Scholar
23.Latham, RH, Stamm, WE. Role of fimbriated Escherichia coli in urinary tract infections in adult women: correlation with localization studies. J Infect Dis 1984; 149: 835–40.CrossRefGoogle ScholarPubMed
24.Silverblatt, FJ, Dreyer, JS, Schauer, S. Effect of pili on susceptibility of Escherichia coli to phagocytosis. Infect Immun 1979; 24: 218–23.CrossRefGoogle ScholarPubMed
25.Ørskov, I, Ferenoz, A, Ørskov, F. Tamm-Horsfall protein on uromucoid is the normal urinary slime that traps type-1 fimbriated Escherichia coli. Lancet 1980; i: 887.CrossRefGoogle Scholar
26.Beachey, EH. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J Infect Dis 1981; 143: 325–45.CrossRefGoogle Scholar
27.Jones, GW. The attachment of bacteria to the surfaces of animal cells. In: Reissig, JL ed. Microbial interactions, receptors and recognition. London: Chapman & Hall, 1977; series B; vol 3: 139–76.CrossRefGoogle Scholar
28.Smith, HW. Microbial surfaces in relation to pathogenicity. Bacteriol Rev 1977; 41: 475–500.CrossRefGoogle ScholarPubMed
29.Jann, K, Schmidt, G, Blumenstoch, E, Vosbeck, K. Escherichia coli adhesion to Saccharomyces cerevisiae and mammalian cells: Role of piliation and surface hydrophobicity. Infect Immun 1981; 484–9.CrossRefGoogle ScholarPubMed
30.Sobel, JD, Obedeanu, N. Role of hydrophobicity in adherence of Gram-negative bacteria to epithelial cells. Ann Clin Lab Sci 1984; 14: 216–24.Google ScholarPubMed
31.Ljungh, Å, Wadström, T. Fimbriation of Escherichia coli in urinary tract infections. Curr Microbiol 1983; 8: 263–8.CrossRefGoogle Scholar