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Characterization of clinically significant isolates of Staphylococcus epidermidis from patients with cerebrospinal fluid shunt infections

Published online by Cambridge University Press:  19 October 2009

J. Etienne ,
B. Charpin ,
J. Grando ,
Y. Brun ,
M. Bes  and
J. Fleurette
J. Etienne
Affiliation:
Centre National de Référence des Staphylocoques, Faculté de Médecine Alexis Carrel, rue Guillaume Paradin, 69372 Lyon Cedex 08, France Hôpital Louis Pradel, Laboratoire de Bactériologie, Faculté de Médecine Lyon-Nord, BP Lyon Montchat, 69394 Lyon Cedex 3, France
B. Charpin
Affiliation:
Hôpital Louis Pradel, Laboratoire de Bactériologie, Faculté de Médecine Lyon-Nord, BP Lyon Montchat, 69394 Lyon Cedex 3, France
J. Grando
Affiliation:
Hôpital Louis Pradel, Laboratoire de Bactériologie, Faculté de Médecine Lyon-Nord, BP Lyon Montchat, 69394 Lyon Cedex 3, France
Y. Brun
Affiliation:
Centre National de Référence des Staphylocoques, Faculté de Médecine Alexis Carrel, rue Guillaume Paradin, 69372 Lyon Cedex 08, France
M. Bes
Affiliation:
Centre d'Etudes et de Recherches Bactériologiques, Institut Pasteur de Lyon, rue Guillaume Paradin, 69372 Lyon Cedex 3, France
J. Fleurette
Affiliation:
Centre National de Référence des Staphylocoques, Faculté de Médecine Alexis Carrel, rue Guillaume Paradin, 69372 Lyon Cedex 08, France
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Biotyping, slime production, antibiograms, extrachromosomal DNA banding and total DNA restriction analysis were used to characterize Staphylococcus epidermidis strains causing cerebrospinal fluid shunt infections in 11 patients. Infections considered to be community acquired and those acquired in the first 2 weeks of hospital admission were due to oxacillin-susceptible isolates. Multiply resistant strains were isolated from patients who were in hospital for more than 1 month before tube implantation. Slime was detected in staphylococci for 54% of cases, but its expression varied. Strains from different patients could be differentiated from one another by the extrachromosomal DNA bandings and total DNA restriction patterns, but isolates from the same patient were usually similar. During the period of external drainage, epidemiological markers were useful in differentiating persistence of infection from contamination or re-infection by a new strain.

Type
Research Article
Information
Epidemiology & Infection , Volume 106 , Issue 3 , June 1991 , pp. 467 - 475
Copyright
Copyright © Cambridge University Press 1991

References

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