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Characterization of human rotavirus strains causing gastroenteritis in Kenya

Published online by Cambridge University Press:  19 October 2009

Z. Gatheru ,
N. Kobayashi ,
N. Adachi ,
S. Chiba ,
J. Muli ,
P. Ogaja ,
J. Nyangao ,
E. Kiplagat  and
P. M. Tukei
Z. Gatheru
Affiliation:
Virus Research Centre, Kenya Medical Research Institute, P.O. Box 54S40, Nairobi, Kenya
N. Kobayashi*
Affiliation:
Department of Hygiene, Sapporo Medical College, S-l, W-17, Chuo-ku, Sapporo, Japan
N. Adachi
Affiliation:
Department of Pediatrics, Sapporo Medical College, S-l, W-16, Chuo-ku, Sapporo, Japan
S. Chiba
Affiliation:
Department of Pediatrics, Sapporo Medical College, S-l, W-16, Chuo-ku, Sapporo, Japan
J. Muli
Affiliation:
Virus Research Centre, Kenya Medical Research Institute, P.O. Box 54S40, Nairobi, Kenya
P. Ogaja
Affiliation:
Virus Research Centre, Kenya Medical Research Institute, P.O. Box 54S40, Nairobi, Kenya
J. Nyangao
Affiliation:
Virus Research Centre, Kenya Medical Research Institute, P.O. Box 54S40, Nairobi, Kenya
E. Kiplagat
Affiliation:
Virus Research Centre, Kenya Medical Research Institute, P.O. Box 54S40, Nairobi, Kenya
P. M. Tukei
Affiliation:
Virus Research Centre, Kenya Medical Research Institute, P.O. Box 54S40, Nairobi, Kenya
*
*Corresponding author.
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Summary

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Human rotavirus strains from Kenya, from children with gastroenteritis in an urban area (Nairobi) and three rural areas were characterized by antigenic and genomic analysis. While in all areas strains with subgroups II and G serotype 1 antigens were most common, two unusual strains were detected. One strain (NK59: subgroup II. G serotype 4) possessed an additional RNA band on polyacrylamide gel electrophoresis, the other (D202) which had antigenic specificity of subgroup II and G serotype 1 showed a ‘short’ RNA pattern. The latter strain was adapted to growth in cell culture.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 2 , April 1993 , pp. 419 - 423
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Az, Kapikian, Rm, Chanock. Rotaviruses. In: Bn, Fields. Dm, Knipe, Rm, Chanock, Ms, Hirsch, Jl, Melnick, It, Monath et al. , eds. Virology. New York: Raven Press, 1990: 1353–404.Google Scholar
2.Hoshino, Y.Wyatt, Rg.Greenberg, Hb.Flores, J.Kapikian, Az.. Serotypic similarity and diversity of human rotavirus of mammalian and avian origin as studied by plaquereduction neutralization. J Infect Dis 1984: 149: 694–702.Google ScholarPubMed
3.Matsumo, Y, Hasegawa, A, Mukoyama, A, Inouve, S. A candidate for a new serotype of human rotavirus. J Virol 1985; 54: 623–4.Google Scholar
4.Hf, Clark, Hoshino, Y, Lm, Bell, Groff, J.Hess, G.Bachman, P. et al. Rotavirus isolate W161 representing a presumptive new human serotype. J Clin Mierobiol 1087: 25: 1757 62.Google Scholar
5.Urasawa, S, Urasawa, T, Wakasugi, F.Kobayashi, N.Taniguchi, K.Lintag, Ic et al. Presumptive seventh serotype of human rotavirus. Arch Virol 1000: 113: 279 82.Google Scholar
6.Gorziglia, M, Larralde, G.AZ, KapikianRm, Chanoek. Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Xatl Acad Sci USA 1990; 87: 7155–9.Google ScholarPubMed
7.Qian, Y, Ky, Green. Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology 1991: 182: 407–12.Google Scholar
8.Nakagomi, O.Nakagomi, T.Hoshino, Y.Flores, J.AZ., KapikianGenetic analysis of human rotavirus that belongs to subgroup I but has an RNA pattern typical of subgroup II human rotaviruses. J Clin Mierobiol 1987: 25: 1159–64.Google ScholarPubMed
10.Kobayashi, N, IC., LintageUrasawa, T.Taniguchi, K, Mc., SanielUrasawa, S.Unusual human rotavirus strains having subgroup 1 specificity and ‘long’ RNA electropherotype. Arch Virol 1990: 112: 27–40.Google Scholar
10.Taniguchi, K, Urasawa, T.Urasawa, S.Yasuhara, T. Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzymelinked immunosorbent assay. J Med Virol 1984; 14: 115–25.Google Scholar
11.Taniguchi, K.Urasawa, T.Morita, Y.Greenberg, Hb.Urasawa, S.Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotypes 1–. 2–. 3–. and 4-spccific monoclonal antibodies to VP7. J. Infect Dis 1987: 15: 1159–66.Google Scholar
12.Mk, Estes, Cohen, J.Rotavirus gene structure and function. Mierobiol Rev 1989: 53: 410–49.Google Scholar
13.Urasawa, T., Urasawa, S.. Taniguchi, K.Sequential passages of human rotavirus in MA-104 cells. Mierobiol Immunol 1981: 25: 1025–35.Google ScholarPubMed
14.Taniguchi, K.Wakasugi, F.Pongsuwanna, Y.Urasawa, T.Ukae, S.Chiba, S.Urasawa, S.Serotyping of human and bovine rotaviruses bv polvmerase chain reaction. Epidemiol Infect 1992; 109: 303–12.Google Scholar
15.Kobayashi, N, Taniguchi, K, Urasawa, S. Identification of operationally overlapping and independent cross-reactive neutralization regions on human rotavirus VP4. J. Gen Virol 1990: 71: 2615–23.Google ScholarPubMed
16.Kobayashi, N., Taniguchi, K.Urasawa, T.Urasawa, S.Preparation and characterization of a neutralizing monoelonal antibody directed at VP4 of rotavirus strain K8 which has unique VP4 neutralization epitopes. Arch Virol 1991: 121: 15362.Google ScholarPubMed