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Characterization of resistance plasmids and carried phages in an epidemic clone of multi-resistant Salmonella typhimurium in India

Published online by Cambridge University Press:  25 March 2010

J. A. Frost ,
B. Rowe ,
L. R. Ward  and
E. J. Threlfall
J. A. Frost
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale Avenue, London, NW9 5HT
B. Rowe
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale Avenue, London, NW9 5HT
L. R. Ward
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale Avenue, London, NW9 5HT
E. J. Threlfall
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale Avenue, London, NW9 5HT
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Hospital outbreaks of severe gastroenteritis caused by multi-resistant Salmonellatyphimurium have occurred in a number of cities throughout India since 1977. The strains involved belong to phage types 66 or 122, or are untypable; the latter are derived from types 66 or 122 by acquisition of one or more of a number of temperate bacteriophages. Types 66 and 122 are closely related and react with the same phages of the S. typhimurium typing scheme.

A plasmid belonging to compatibility group FIme encoding resistance to ampicillin, chloramphenicol, kanamycin, streptomycin, sulphonamides, spectinomycin, tetracyclines, gentamicin and trimethoprim (R-type ACKSSuSpTGTm) is present in all of the multi-resistant strains. Several other plasmids have been identified including an SSu resistance determinant, a group I2, transfer factor and an R factor coding for resistance to kanamycin, streptomycin and sulphonamides which is compatible with plasmids of all the standard compatibility groups. These plasmids are only present in a proportion of the strains examined.

Examination of strains from other sources has identified a paediatric hospital outbreak in Saudi Arabia and a number of sporadic infections in Great Britain which have been caused by the same organisms. These studies show that, despite differences in phage type and plasmid content, this group of strains belongs to a single clone which has become widespread in India with some extension to other countries.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 193 - 204
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Agarwal, K. C., Garg, R. K., Panhotra, B. R., Verma, A. D., Ayyagari, A. & Mahanta, J. (1980). Drug resistance in Salmonellae isolated at Chandigarh (India) during 1972–1978. Antonie van Leeuwenhoek 146, 383–390.CrossRefGoogle Scholar
Ahuja, S., Mago, M. L., Singha, H. & Saxena, S. N. (1979). Multiple drug resistance patterns of Salmonella strains in India during 1976. Indian Journal of Medical Research 70, 702–709.Google ScholarPubMed
Anderson, E. S. & Threlfall, E. J. (1974). The characterisation of plasmids in the Enterobacteria. Journal of Hygiene 72, 471–487.CrossRefGoogle ScholarPubMed
Anderson, E. S., Threlfall, E. J., Carr, J. M., McConnell, M. M. & Smith, H. R. (1977 a). Clonal distribution of resistance plasmid-carrying Salmonella typhimurium mainly in the Middle East. Journal of Hygiene 79, 425–448.CrossRefGoogle ScholarPubMed
Anderson, E. S., Ward, L. R., de Saxe, M. J. & de Sa, J. D. H. (1977 b). Bacteriophage-typing designations of Salmonella typhimurium. Journal of Hygiene 78, 297–300.CrossRefGoogle ScholarPubMed
Callow, B. R. (1959). A new phage-typing scheme for Salmonella typhimurium. Journal of Hygiene 57, 346–359.CrossRefGoogle Scholar
Diarrhoeal Diseases Control Programme (1979). Multiresistant strains of Salmonella typhimurium. WHO Weekly Epidemiological Record 40, 310–311.Google Scholar
Frost, J. A., de Saxe, M. J. & Anderson, E. S. (1976). Distribution of subtypes of Salmonella typhimurium phage type 193 and associated drug resistance transfer systems. Proceedings of the Society for General Microbiology 3, 88.Google Scholar
Grindley, N. D. F., Grindley, J. N.& Anderson, E. S. (1972). R factor compatibility groups. Molecular and General Genetics 119, 287–297.CrossRefGoogle ScholarPubMed
Hedges, R. J. & Jacob, A. E. (1974). Transposition of ampicillin resistance from RP4 to other replicons. Molecular and General Genetics 132, 31–40.CrossRefGoogle ScholarPubMed
Jacob, A. E., Shapiro, J. A., Yamamoto, L., Smith, D. I., Cohen, S. N. & Berg, D. (1977). Appendix B. Bacterial Plasmids. In DNA Insertion Elements, Plasmids and Episomes (ed. Bukhari, A. I.Shapiro, J. A. and Adhya, S. L.), pp. 607–670. Cold Spring Harbor Laboratory.Google Scholar
McConnell, M. M., SMITH, H. R., Leonardopoulis, J. & Anderson, E. S. (1979). The value of plasmid studies in the epidemiology of infections due to drug-resistant Salmonella wien. Journal of Infectious Diseases 139, 178–189.CrossRefGoogle ScholarPubMed
Rowe, B., Frost, J. A., Threlfall, E. J. & Ward, L. R. (1980). Spread of a multiresistant clone of Salmonella typhimurium phage type 66/122 in S.E. Asia and the Middle East. Lancet i, 1070–1071.CrossRefGoogle Scholar
Threlfall, E. J., Ward, L. R. & Rowe, B. (1978). Spread of multiresistant strains of Salmonella typhimurium phage types 204 and 193 in Britain. British Medical Journal ii, 997.CrossRefGoogle Scholar
WHO (1974). Transferable drug resistance in salmonellae in South and Central America. WHO Weekly Epidemiological Record 8, 65–69.Google Scholar
Willshaw, G. A., Smith, H. R. & Anderson, E. S. (1978). Molecular studies of FIme resistance plasmids particularly in epidemic Salmonella typhimurium. Molecular and General Genetics 159 111–116.CrossRefGoogle ScholarPubMed