Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-14T04:37:50.649Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of haemagglutination-inhibition and single radial haemolysis techniques for detecting antibodies to influenza A and B viruses

Published online by Cambridge University Press:  19 October 2009

G. Mancini ,
I. Donatelli ,
G. Arangio-Ruiz ,
C. Rozera  and
T. Macchia
G. Mancini
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
I. Donatelli
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
G. Arangio-Ruiz
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
C. Rozera
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
T. Macchia
Affiliation:
Departments of Clinical Biochemistry, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The sensitivities of haemagglutination-inhibition (HI) and single radial haemolysis (SRH) techiques in detecting antibodies against influenza A/Bangkok/1/79, A/Brazil/11/78, B/Singapore/222/79, B/Hong Kong/5/72 strains, in human sera were compared. For antibodies to influenza B viruses the HI tests employing ether-treated antigens were also evaluated. The SRH technique appears to be more sensitive for detecting protective titres of antibodies against influenza B strains and influenza A/Brazil/11/78.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 1 , August 1983 , pp. 157 - 162
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Advanced Laboratory Techniques for Influenza Diagnosis. (1975). Department Health Education and Welfare, Atlanta.Google Scholar
Berlin, B. S., McQueen, J., Minuse, E. & Davenport, F. M. (1963). A method for increasing the sensitivity of the haemagglutination-inhibition test with equine influenza virus. Virology 21, 665–666.Google Scholar
Chakraverty, P. (1980). Comparison of haemagglutination-inhibition and single-radial-haemolysis techniques for detection of antibodies to influenza B virus. Archives of Virology 63, 285–289.Google Scholar
Draper, N. R. & Smith, H. (1966). Weighted Least Squares (Applied Regression Analysis), pp. 77–81. New York: J. Wiley.Google Scholar
Monto, A. S. & Maassab, H. F. (1981). Ether-treatment of type B influenza antigen for the haemagglutination-inhibition test. Journal of Clinical Microbiology 13, 54–57.Google Scholar
Oxford, J. S., Yetts, R. & Schild, G. C. (1982). Quantitation and analysis of the specificity of post-immunization antibodies to influenza B viruses using single radial haemolysis. Journal of Hygiene 88, 325–333.CrossRefGoogle ScholarPubMed
Pettersson, R. S. (1980). Correlation between antibody titers to influenza A virus subtypes H1N1 and H3N2 as measured by haemagglutination-inhibition and haemolysis-in-gel tests. Journal of Infectious Diseases 142, 450.Google Scholar
Schild, G. C. & Dowdle, W. R. (1975). Characterization and diagnositic serology. In The Influenza Viruses and Influenza (ed. Kilbourne, E. D.), p. 324. New York: Academic Press.Google Scholar
Schild, G. C., Pereira, M. S. & Chakraverty, P. (1975). Single radial haemolysis: a new method for assay of antibodies to influenza haemagglutinin. Bulletin of the World Health Organization 52, 43–50.Google ScholarPubMed