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Correlation between esterase electrophoretic polymorphism and virulence–associated traits in extra–intestinal invasive strains of Escherichia coli

Published online by Cambridge University Press:  15 May 2009

Ph. Goullet ,
B. Picard ,
M. Contrepois ,
J. De Rycke  and
J. Barnouin
Ph. Goullet*
Affiliation:
Laboratoire de Microbiologie, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
B. Picard
Affiliation:
Laboratoire de Microbiologie, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
M. Contrepois
Affiliation:
Institute National de la Recherche Agronomique, Laboratoire de Microbiologie, Centre de Recherche de Clermont–Ferrand–Theix, Saint Genès Champanelle, France
J. De Rycke
Affiliation:
Institut National de la Recherche Agronomique, Station de Pathologie de la Reproduction, Centre de Recherche de Tours–Nouzilly, Monnaie, France
J. Barnouin
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire d'ecopathologie, Centre de Recherche de Clermont–Ferrand–Theix, Saint Genès Champanelle, France
*
*Correspondence: Ph. Goullet, Service de Microbiologie, Hôpital Beaujon (Faculté de Médecine Xavier Bichat, Université Paris 7), 100 Bd du General Leclerc, 92110 Clichy, France.
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The electrophoretic variations of carboxylesterase B and of esterases A, C and I, the presence of mannose resistant haemagglutinin, α–haemolysin, cytotoxic necrotizing factor type 1 (CNF1) and certain O antigens were compared in 150 strains of Escherichia coli responsible for extra-intestinal infections. Electrophoretic mobilities of outer membrane proteins (OMP) were also studied for strains belonging to O4, O6, O7, O8 and O75 serogroups. Fast migrating allozymes of carboxylesterase B (pattern B1) were correlated with slow migrating allozymes of esterase C, serogroups O7 and O8, lack of virulence factor, and particular OMP patterns, whereas slow migrating allozymes of carboxylesterase B (pattern B2) were correlated with fast migrating allozymes of esterase C, serogroups O2, O4, O6, O18 and O75, virulence factor production, and distinct OMP patterns. Allozymes of esterases A and I were not clearly correlated with the distribution of virulence factors. The pattern B2 was more strongly associated with CNF1 than with α–haemolysin and mannose resistant haemagglutinin. These results substantiate the view that the electrophoretic pattern B2 of carboxylesterase B identified most of the highly pathogenic strains implicated in extra-intestinal infection of humans.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 1 , February 1994 , pp. 51 - 62
Copyright
Copyright © Cambridge University Press 1994

References

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