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Cytomegalovirus excretion in gnotobiotic pigs

Published online by Cambridge University Press:  15 May 2009

N. Edington ,
R. G. Watt  and
W. Plowright
N. Edington
Affiliation:
Department of Pathology, Royal Veterinary College London, NW1 0TU
R. G. Watt
Affiliation:
Department of Pathology, Royal Veterinary College London, NW1 0TU
W. Plowright
Affiliation:
Department of Pathology, Royal Veterinary College London, NW1 0TU
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Summary

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Germ-free piglets were infected intranasally with porcine cytomegalovirus (PCMV) at 1 day (group A) or 3 weeks of age (group B). Viraemia and virus excretion by the nasal, pharyngeal and conjunctival routes was studied up to the time of death or to 12 weeks. Virus was also sought in tissues at death or at slaughter, as well as in a few urine samples.

Viraemia was detected in group A between days 5 and 19 after infection and in group B between days 14 and 16 inclusive. The chief route of virus excretion was the nasal mucosa, followed by the pharynx and conjunctiva; the maximal duration of excretion by these routes was 32, 25 and 14 days for pigs of group A and 9, 7 and 4 days for group B. The quantity of virus was also greater in the former group, of which 3 died of generalized PCMV infection.

A viruria was demonstrated in 2 animals.

Antibody detectable in indirect immunofluorescence (IIF) tests appeared towards the end of the third week, reaching maximal titres at 5 to 7 weeks after infection. The mean peak titre of antibody in group B was lower than in group A.

Corticosteroid treatment at days 56–62 after infection resulted in some recrudescence of virus excretion, accompanied in group B by about a twofold increase in IIF antibody. PCMV was isolated in cultures of lung macrophages from 4 of 7 animals killed at about 12 weeks after inoculation.

Type
Research Article
Information
Journal of Hygiene , Volume 77 , Issue 2 , October 1976 , pp. 283 - 290
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Corner, A. H., Mitchell, D., Julian, R. J. & Meads, E. B. (1964). A generalised disease in piglets associated with the presence of cytomegalic inclusions. Journal of Comparative Pathology 74, 192.Google Scholar
Edington, N., Plowright, W. & Watt, R. G. (1976). Generalised porcine cytomegalic inclusion disease: distribution of cytomegalic cells and virus. Journal of Comparative Pathology 86, 191.CrossRefGoogle ScholarPubMed
Henson, D., Smith, G. R., Gehrike, J. & Neapolitan, C. (1967). Effect of cortisone on non-fatal mouse cytomegalovirus infection. American Journal of Pathology 51, 1001.Google Scholar
Lang, D. J. (1972). Cytomegalovirus infections in organ transplantation and post transfusion. Archiv für die gesamte Virusforschung 37, 365.CrossRefGoogle ScholarPubMed
Levinsohn, E. M., Foy, H. M., Kenny, G. E., Wentworth, B. B. & Grayston, J. T. (1969). Isolation of CMV from a cohort of 100 infants throughout the first year of life. Proceedings of the Society for Experimental Biology and Medicine 132, 957.Google Scholar
Medearis, D. N. (1964). Mouse cytomegalovirus infection. II. Observations during prolonged infections. American Journal of Hygiene 80, 103.Google Scholar
Numazaki, Y., Yano, N., Morizuka, T., Takai, S. & Ishida, N. (1970). Primary infection with human cytomegalovirus: virus isolation from healthy infants and pregnant women. American Journal of Epidemiology 91, 410.CrossRefGoogle ScholarPubMed
Plowright, W., Edington, N. & Watt, R. G. (1976). The behaviour of porcine cytomegalovirus in commercial pig herds. Journal of Hygiene 75, 125.Google Scholar
Plummer, G. (1973). Cytomegaloviruses of man and animals. Progress in Medical Virology 15, 92.Google Scholar
Rowe, W. P., Hartley, J. W., Cramblett, J. G. & Mastrota, F. M. (1958). Detection of human salivary gland virus in the mouth and urine of children. American Journal of Hygiene 67, 57.Google ScholarPubMed
Sheffey, B. E. & Davies, D. H. (1972). Reactivation of bovine herpes virus after corticosteroid treatment. Proceedings of the Society for Experimental Biology and Medicine 140, 1974.Google Scholar
Trexler, P. C. (1971). Microbiological isolation of large animals. Veterinary Record 88, 15.CrossRefGoogle ScholarPubMed
Watt, R. G., Plowright, W., Sabo, A. & Edington, B. (1973). A sensitive cell culture system for the virus of porcine inclusion body rhinitis. Research in Veterinary Science 14, 119.Google Scholar