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Detection of Legionella pneumophila in environmental water samples using a fluorescein conjugated monoclonal antibody

Published online by Cambridge University Press:  15 May 2009

T. Makin  and
C. A. Hart
T. Makin
Affiliation:
Department of Medical Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
C. A. Hart
Affiliation:
Department of Medical Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
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Summary

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Sixty-three environmental water samples from various sources were examined for the presence of Legionella pneumophila with a commercially available direct fluorescent monoclonal antibody (GS), an indirect fluorescent antibody test (IFAT) and culture. GS detected L. pneumophila in 94% and 100% of environmental water samples which were culture and IFAT positive for L. pneumophila, respectively. IFAT detected 69% of L. pneumophila culture positive samples. Cultures of L. pneumophila serogroups 1 to 12, 14 and non L. pneumophila bacteria which may be found in water, and bacteria containing non-specific binding proteins, were stained by GS and IFAT. GS identified all serogroups of L. pneumophila and did not cross react with any non-L. pneumophila bacteria. L. pneumophila in environmental samples was easy to detect against a clear dark background when stained with GS.

Type
Special Article
Information
Epidemiology & Infection , Volume 103 , Issue 1 , August 1989 , pp. 105 - 112
Copyright
Copyright © Cambridge University Press 1989

References

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