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Development of an enzyme-linked immunosorbent assay (ELISA) for the detection of specific antibodies against an H7N7 and an H3N8 equine influenza virus

Published online by Cambridge University Press:  19 October 2009

M. S. Denyer ,
J. R. Crowther ,
R. C. Wardley  and
R. Burrows
M. S. Denyer
Affiliation:
Animal Virus Research Institute, Pirbright, Woking, Surrey
J. R. Crowther
Affiliation:
Animal Virus Research Institute, Pirbright, Woking, Surrey
R. C. Wardley
Affiliation:
Animal Virus Research Institute, Pirbright, Woking, Surrey
R. Burrows
Affiliation:
Animal Virus Research Institute, Pirbright, Woking, Surrey
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This paper describes a solid-phase microtitre plate enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to equine influenza viruses. Using egg-grown influenza viruses as the antigens attached to the solid phase, crossreactions were observed between an H7N7 equine virus (designated A1) and an H3N8 equine influenza virus (designated A2) when untreated antisera were tested. Absorption of antisera with egg-grown A/Porcine/Shope/1/33 influenza virus eliminated cross-reactive antibodies so that specific detection of anti-equine influenza A1 or A2 antibodies was possible.

Examination of horse sera following vaccination with A1 and/or A2 isolates showed that antibodies were produced against antigen associated with egg allantoic fluid as well as against virus. Such antibodies were eliminated following the absorption of antisera with porcine influenza virus. Results using sera from horses with known vaccination histories confirmed that the ELISA preferentially detected antibodies homologous to the antigen attached to the solid phase and methods to evaluate the current serological state of individual horses by relating the titres of specific antibodies against equine influenza A1 and A2 isolates are shown. This ELISA provides a simple and rapid method of assessing specific antibodies from horse sera and offers advantages over the ‘routine’ HI and SRH assessments since it gives high precision, is economical of reagents and has the capacity to handle large numbers of serum samples.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 3 , December 1984 , pp. 609 - 620
Copyright
Copyright © Cambridge University Press 1984

References

Abu Elzein, E. M. E. & Crowther, J. R. (1982). Differentiation of foot-and-mouth disease virus strains using a competition enzyme-linked immunosorbent assay. Journal of Virological Methods 3, 355–365.CrossRefGoogle ScholarPubMed
Bürki, F. & Sibalin, M. (1973). Standardization of the haemagglutination-inhibition test for two equine influenza viruses. Zentralblall für Bakteriologie Parasiienkunde Infektionskrankheiten und Hygiene (Abt. a: Orig. Reihe A) 223, 163–172.Google ScholarPubMed
Burrows, R. & Denyer, M. (1982). Antigenic properties of some equine influenza viruses. Archives of Virology 73, 15–24.CrossRefGoogle ScholarPubMed
John, T. J. & Fulginiti, V. A. (1966). Para influenza 2 virus: increase in haemagglutinin titre on treatment with Tween 80 and ether. Proceedings of the Society of Experimental Biology, N. Y. 121, 100–111.Google Scholar
Nakane, P. L. & Kawaoi, A. (1974). Peroxidase-labelled antibody. A new method of conjugation. Journal of Histochemistry and Cytochemistry 22, 1084–1001.CrossRefGoogle Scholar
Palmer, D. F., Coleman, M. T., Dowdle, W. R. & Sohild, G. C. (1975). Advanced Laboratory Techniques for Influenza Diagnosis. Immunology Series 6, Procedural Guide. U.S. Dept. Health Ed. and Welfare.Google Scholar
Schild, G. C., Pereira, M. S. & Chakraverty, P. (1975). Single-radialhaemolysis: a new method for the assay of antibody to influenza haemagglutinin. WHO Bulletin 52, 43–50.Google ScholarPubMed