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The Distribution of Antibody to Crystalline Egg Albumin in the Serum of Injected Rabbits

Published online by Cambridge University Press:  15 May 2009

Muriel E. Adair  and
G. L. Taylor
Muriel E. Adair
Affiliation:
From the Department of Pathology, University of Cambridge
G. L. Taylor
Affiliation:
From the Department of Pathology, University of Cambridge
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Two batches of pooled antisera obtained from rabbits injected with crystalline egg albumin have been fractionated with ammonium sulphate in order to investigate the distribution of the antibody to this antigen in the serum proteins.

The albumin fractions contained no antibody.

All the globulin fractions examined contained antibody, and flocculated readily when mixed with appropriate dilutions of the antigen.

The distribution of antibody throughout the serum globulin was fairly uniform, the ratio of the antibody content to the protein concentration being the smallest in a fraction with a solubility in ammonium sulphate slightly lower than that of pseudoglobulin.

Type
Research Article
Information
Journal of Hygiene , Volume 36 , Issue 4 , October 1936 , pp. 564 - 569
Copyright
Copyright © Cambridge University Press 1936

References

Adair, G. S. & Robinson, Muriel E. (1930). The specific refraction increments of serum-albumin and serum-globulin. Biochem. J. 24, 993.CrossRefGoogle ScholarPubMed
Boyd, W. C. & Hooker, S. B. (1934). The influence of the molecular weight of the antigen on the proportion of antibody to antigen in precipitates. J. Gen. Physiol. 17, 341.CrossRefGoogle ScholarPubMed
Culbertson, J. T. (1935). The role of the precipitin antibody in the removal of intravenously injected antigen. J. Immunol. 28, 277.CrossRefGoogle Scholar
Dean, H. R. & Webb, R. A. (1926). The influence of optimal proportions of antigen and antibody in the serum precipitation reaction. J. Path. Bact. 29, 473.Google Scholar
Heidelberger, M. & Kendall, F. E. (1934). The role of multiple reactive groups in antigen-antibody union as illustrated by an instance of cross precipitation. J. Exp. Med. 59, 519.CrossRefGoogle ScholarPubMed
Hooker, S. B. & Boyd, W. C. (1935). Equivalence-point ratio of antibody to antigen in ovalbumin precipitates. Proc. Soc. Biol. Med. 32, 1104.CrossRefGoogle Scholar
Parnas, J. K. & Wagner, R. (1921). Ueber die Ausfuehrung von Bestimmungen kleiner Stickstoffmengen nach Kjeldahl. Biochem. Z. 125, 253.Google Scholar
Taylor, G. L., Adair, G. S. & Adair, Muriel E. (1932). The estimation of proteins by the precipitation reaction. J. Hygiene, 32, 340.CrossRefGoogle ScholarPubMed
Taylor, G. L., Adair, G. S. & Adair, Muriel E. (1934). Optimal proportions, neutrality and maximal precipitation in mixtures of albumin and antiserum. Ibid. 34, 118.Google Scholar