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Electrophoretic typing of Enterobacter cloacae with a limited set of enzyme stains

Published online by Cambridge University Press:  15 May 2009

M. A. Gaston  and
M. Warner
M. A. Gaston
Affiliation:
Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
M. Warner
Affiliation:
Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
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Summary

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Hospital isolates of Enterobacter cloacae were analysed by polyacrylamide gel electrophoresis for enzyme polymorphism and the results were compared with established serotyping, phage typing and biotyping techniques. Initially, the diversity of electromorphs of 13 enzymes was determined on a representative set of 62 distinct strains. Two broad clusters of strains were found in the species, and analysis by serotype suggested a limited diversity within the most frequent o serotvpes. A subset of three enzymes, lactate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase and an unidentified marker, were selected and used to type groups of hospital isolates. There was good general agreement between the two systems, although the enzyme method failed to distinguish between some strains with the same serotype. This method provided useful epidemiological information and, in the absence of established typing systems, it is a practical approach to subdividing the species.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 2 , October 1989 , pp. 255 - 264
Copyright
Copyright © Cambridge University Press 1989

References

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