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Enterotoxin production by strains of Staphylococcus aureus isolated from clinical and non-clinical specimens with special reference to enterotoxin F and toxic shock syndrome

Published online by Cambridge University Press:  25 March 2010

Marianne P. De Nooij ,
Wijnanda J. van Leeuwen  and
Serve Notermans
Marianne P. De Nooij
Affiliation:
Laboratory for Zoonoses and Food Microbiology and Laboratory for Bacteriology. National Institute of Public Health. P.O. Box 1.Bilthoven, The Netherlands
Wijnanda J. van Leeuwen
Affiliation:
Laboratory for Zoonoses and Food Microbiology and Laboratory for Bacteriology. National Institute of Public Health. P.O. Box 1.Bilthoven, The Netherlands
Serve Notermans
Affiliation:
Laboratory for Zoonoses and Food Microbiology and Laboratory for Bacteriology. National Institute of Public Health. P.O. Box 1.Bilthoven, The Netherlands
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Summary

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Enterotoxin production by strains of Staphylococcus aureus isolated from clinical specimens of human and animal origin and from healthy human carriers was investigated. All nine patients admitted to hospital with symptoms of toxic shock syndrome (TSS) yielded enterotoxin-producing strains of S. aureus. Eight of these produced staphylococcal enterotoxin F (SEF). A significantly smaller proportion of strains (42% of 50 strains tested) isolated from other clinical specimens of hospitalized patients produced SEF. Production of SEF by strains isolated from clinical specimens of animal origin (48 strains) was not observed. Twenty-nine per cent of 24 S. aureus strains isolated from noses of hospital staff produced SEF. This result was not significantly different from that obtained from strains isolated from clinical specimens other than TSS. A similar percentage of strains isolated from healthy human carriers outside hospital produced SEF (25% of 24 strains tested).

The results indicated that enterotoxin production, especially that of SEF. is associated with S. aureus isolated from patients suspected of TSS. There was no indication of an association between S. aureus isolated from other staphylococcal infections and SEF production.

All strains were phage typed and 79%, of the strains belonging to the international phage-group I produced SEF. All strains lysed by phage 187 were found to produce SEF.

Type
Research Article
Information
Journal of Hygiene , Volume 89 , Issue 3 , December 1982 , pp. 499 - 505
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Baird-Parker, A. C. (1971). Factors affecting the production of bacterial food poisoning toxins. Journal of Applied Bacteriology 34, 181–197.CrossRefGoogle ScholarPubMed
Bergdoll, M. S., Crass, B. A., Reiser, R: F., Robbins, R. N. & Davis, J. P. (1981). A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphy-lococcus aureus isolates. The Lancet, i, 1017–1021.CrossRefGoogle Scholar
Berman, R. E., Gilpin, R. W. & Knight, R. A. (1981). Enterotoxin synthesis by clinical isolates of Staphylococci. Laboratory Medicine 12, 621–622, 654.CrossRefGoogle Scholar
Brückler, H., Klima, M., Schaeg, W., Manz, D. & Blobel, M. (1981). Staphylococcus aureus von Kühen mit subklinischen Euerinfektionen. Zentralblatt für Veterinärmedizin; Reihe B 28, 494–499.CrossRefGoogle Scholar
Casman, E. P., Bennet, R. W., Dorsey, A. E. & Issa, J. A. (1967). Identification of a fourth Staphylococcal enterotoxin, enterotoxin D. Journal of Bacteriology 94, 1875–1882.CrossRefGoogle ScholarPubMed
Davis, J. P., Chesney, P. J., Wand, P. & La Venture, M. (1980). Toxic-shock-syndrome: epidemiologic features recurrence, risk factors and prevention. New England Journal of Medicine 303, 1429–1435.CrossRefGoogle ScholarPubMed
Donelly, C. B., Leslie, J. E., Black, L. A. & Lewis, K. U. (1967). Serological identification of enterotoxigenic staphylococci from cheese. Applied Microbiology 15, 1382–1387.CrossRefGoogle Scholar
Notermans, S. & Dufrenne, J. (1982). A simple purification method for enterotoxin F produced by Staphylococcus aureus and some properties of the toxin. Antonie van Leeuwenhoek. (In the Press.)CrossRefGoogle Scholar
Ouchterlony, O. (1958). Diffusion-in-gel in methods from immunological analysis. Progress in Allergy 5, 1–77.Google ScholarPubMed
Report Subcommittee Staphylococcal Phage-Typing (1975). National Journal of Systematic Bacteriology 25, 241–242.CrossRefGoogle Scholar
Robbins, R., Gould, S. & Bergdoll, M. S. (1974). Detecting the enterotoxigenity of Staphylococcus aureus strains. Applied Microbiology 28, 946–950.CrossRefGoogle Scholar
Schlievert, P.M., Shands, K., Dan, B. B., Schmid, G. P. & Nishimura, R. D. (1981). Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock-syndrome. Journal of Infectious Diseases 143, 509–516.CrossRefGoogle ScholarPubMed
Shiozawa, K., Kato, E. & Shimiza, A. (1980). Enterotoxigenity of Staphylococcus aureus strains isolated from chickens. Journal of Food Protection 43, 683–685.CrossRefGoogle Scholar
Todd, J., Fishaut, M., Kapral, M. & Welch, T. (1978). Toxic-shock-syndrome associated with phage group I staphylococci. Lancet ii, 1116–1118.CrossRefGoogle Scholar
van Leeuwen, W. J. & Rost, J. A. (1976). An additional set of phages for the typing of Staphylococcus aureus strains of human origin and non-typable with the international basic set of phages. Zentralblatt für Bakteriologie Mikrobiologie und Hygiene, I. Abteilung. Supplement Heft 5, 1013–1019.Google Scholar
Wieneke, A. A. (1974). Enterotoxin production by strains of Staphylococcus aureus isolated from foods and human beings. Journal of Hygiene 73, 255–261.CrossRefGoogle ScholarPubMed