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Experimental colonization of broiler chicks with Campylobacter jejuni

Published online by Cambridge University Press:  19 October 2009

S. Shanker ,
A. Lee  and
T. C. Sorrell
S. Shanker
Affiliation:
Bacteriology Department, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, N.S.W. 2145, Australia
A. Lee
Affiliation:
School of Microbiology, University of New South Wales, P.O. Box 1, Kensington, N.S.W. 2033, Australia
T. C. Sorrell
Affiliation:
Infectious Diseases Unit, Westmead Hospital and Department of Medicine, University of Sydney, Westmead, N.S.W. 2145, Atistralia
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Minimal colonization inocula for two broiler strains of Campylobacter jejuni were determined in broiler chicks aged 2–3 days and 2 weeks. Individually housed chicks were exposed to a single oral or cloacal challenge. Diarrhoeal symptoms were absent in all 380 chicks included in the study. Chick susceptibility to the two C. jejuni strains varied. Colonization was effected by < 102−104 colony forming units (c.f.u.) via cloacal challenge and 104–106 c.f.u. via the oral route. Colonization inocula for 2-to 3-day and 2-week-old chicks were similar. Treatment of 1-day-old chicks with fresh adult caecal flora or an anaerobic broth culture of adult caecal flora did not inhibit colonization after challenge with low-dose C. jejuni. Susceptible chicks were colonized rapidly. C. jejuni was detected in 167 of 189 (88%) colonized chicks within 3 days of challenge and persisted during the 2-week monitoring period. Our data suggest that colonization of broiler chicks with C. jejuni is effected more easily by the cloacal than the oral route and is independent of age.

Type
Research Article
Information
Epidemiology & Infection , Volume 100 , Issue 1 , February 1988 , pp. 27 - 34
Copyright
Copyright © Cambridge University Press 1988

References

REFERENCES

Barnes, E. M. (1979). The intestinal microflora of poultry and game birds during life and after storage. Journal of Applied Bacteriology 46, 407–419.CrossRefGoogle ScholarPubMed
Barnes, E. M., Impey, C. S. & Stevens, B. J. H. (1979). Factors affecting the incidence and anti-salmonella activity of the anaerobic caecal flora of the young chick. Journal of Hygiene 82, 263–283.CrossRefGoogle ScholarPubMed
Brouwer, R., Mertens, M. J. A., Siem, T. H. & Katchaki, J. (1979). An explosive outbreak of campylobacter enteritis in soldiers. Antonie van Leeuwenhoek 45, 517–519.CrossRefGoogle ScholarPubMed
Brownell, J. R., Sadler, W. M. & Fanelli, M. J. (1969). Factors influencing the intestinal infection of chickens with Salmonella typhimirium. Avian Diseases 13, 804–816.CrossRefGoogle Scholar
Christenson, B., Ringner, A., Blucher, C., Billaudelle, H., Gundtoft, K. N., Eriksson, G. & Bottiger, M. (1983). An outbreak of campylobacter enteritis among the staff of a poultry abattoir in Sweden. Scandinavian Journal of Infectious Diseases 15, 167–172.CrossRefGoogle ScholarPubMed
Genigeorgis, C., Hassuneh, M. & Collins, P. (1986). Campylobacter jejuni infection on poultry farms and its effect on poultry meat contamination during slaughtering. Journal of Food Protection 49, 895–903.CrossRefGoogle ScholarPubMed
Harris, N. V., Thompson, D., Martin, D. C. & Nolan, C. M. (1986). A survey of campylobacter and other bacterial contaminants of pre-market chicken and retail poultry and meats, King County, Washington. American Journal of Public Health 76, 401–406.CrossRefGoogle ScholarPubMed
Harris, N. V., Weiss, N. S. & Nolan, C. M. (1986). The role of poultry and meats in the etiology Campylobacter jejuni/coli enteritis. American Journal of Public Health 76, 407–411.CrossRefGoogle ScholarPubMed
Hopkins, R. S. & Scott, A. S. (1983). Handling raw chicken as a source for sporadic Campylobacter jejuni infections. The Journal of Infectious Diseases 148, 770.CrossRefGoogle ScholarPubMed
Kapperud, G., Lassen, J., Lauwers, S. & Rosef, O. (1984). Serotyping and biotyping of Campylobacter jejuni and Campylobacter coli from sporadic cases and outbreaks in Norway. Journal of Clinical Microbiology 19, 157–16O.CrossRefGoogle ScholarPubMed
Leaney, N., Cooper, G. N., Jackson, G. D. F. (1978). Percloacal infection of chickens with Salmonella typhimurium. Veterinary Microbiology 3, 155–165.CrossRefGoogle Scholar
Lindblom, G. B., Sjogren, E. & Kaijser, B. (1986). Natural campylobacter colonisation in chickens raised under different environmental conditions. Journal of Hygiene 96, 385–391.CrossRefGoogle ScholarPubMed
Lloyd, A. B., Cumming, R. B. & Kent, R. D. (1977). Prevention of Salmonella typhimurium infection in poultry by pretreatment of chickens and poults with intestinal extracts. Australian Veterinary Journal 53, 82–87.CrossRefGoogle ScholarPubMed
Manninen, K. I., Prescott, J. F. & Dohoo, I. R. (1982). Pathogenicity of Campylobacter jejuni isolates from animals and humans. Infection and Immunity 38, 46–52.CrossRefGoogle Scholar
Meynell, G. C. (1963). Antibacterial mechanisms of the mouse gut II. The role of En and volatile fatty acids in the normal gut. British Journal of Experimental Pathology 44, 209–219.Google Scholar
Montrose, M. S., Shane, S. M. & Harrington, K. S. (1985). Role of litter in the transmission of Campylobacter jejuni. Avian Diseases 29, 392–399.CrossRefGoogle ScholarPubMed
Neill, S. D., Campbell, J. N. & Greene, J. A. (1984). Campylobacter species in broiler chickens. Avian Pathology 13, 777–785.CrossRefGoogle ScholarPubMed
Norkrans, G. & Svedhem, A. (1982). Epidemiological aspects of Campylobacter jejuni enteritis. Journal of Hygiene 89, 163–170.CrossRefGoogle ScholarPubMed
Oosterom, J., Notermans, S., Karman, H. & Engel, G. B. (1983). Origin and prevelance of Campylobacter jejuni in poultry processing. Journal of Food Protection 46, 339–344.CrossRefGoogle Scholar
Oosterom, J., Uyl, C. H. dEN., Banffer, J. R. J. & Huisman, J. (1984). Epidemiological investigations on Campylobacter jejuni in households with a primary infection. Journal of Hygiene 93, 325–332.CrossRefGoogle ScholarPubMed
Penner, J. L. & Hennessy, J. N. (1980). Passive haemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. Journal of Clinical Microbiology 12, 732–737.CrossRefGoogle ScholarPubMed
Rantala, M. & Nurmi, E. (1973). Prevention of the growth of Salmonella infantis in chicks by the flora of the alimentary tract of chickens. British Poultry Science 14, 627–630.CrossRefGoogle ScholarPubMed
Rosenfield, J. A., Arnold, G. J., Davey, G. R., Archer, R. S. & Woods, W. H. (1985). Serotyping of Campylobacter jejuni from an outbreak of enteritis implicating chicken. Journal of Infection 11, 159–165.CrossRefGoogle ScholarPubMed
Ruis-Palacios, G. M., Escamilla, E. & Torres, N. (1981). Experimental Campylobacter diarrhoea in chickens. Infection and Immunity 34, 250–255.CrossRefGoogle Scholar
Sanyal, S. C., Islam, K. M. N., Neogy, P. K. B., Islam, M., Speelman, P. & Huq, M. I. (1984). Campylobacter jejuni diarrhoea model in infant chickens. Infection and Immunity 43, 931–936.CrossRefGoogle ScholarPubMed
Seuna, E., Nagaraja, K. V. & Pomeroy, B. S. (1985). Gentamicin and bacterial culture (Nurmi culture) treatments either alone or in combinations against experimental Salmonella hadar infection in turkey poults. Avian Diseases 29, 617–629.CrossRefGoogle ScholarPubMed
Shanker, S., Rosenfield, J. A., Davey, G. R. & Sorrell, T. C. (1982). Campylobacter jejuni: incidence in processed boilers and biotype distribution in human and broiler isolates. Applied and Environmental Microbiology 43, 1219–1220.CrossRefGoogle Scholar
Shanker, S., Lee, A. & Sorrell, T. C. (1986). Campylobacter jejuni in broilers: the role of vertical transmission. Journal of Hygiene 96, 153–159.CrossRefGoogle ScholarPubMed
Skirrow, M. B. (1977). Campylobacter enteritis; a ‘new’ disease. British Medical Journal 2, 9–11.CrossRefGoogle ScholarPubMed
Skirrow, M. B. & Benjamin, J. (1980). Differentiation of enteropathogenic campylobacters. Journal of Clinical Pathology 33, 1122.CrossRefGoogle Scholar
Smitherman, R. E., Genigeorigis, C. A. & Farver, T. B. (1984). Preliminary observations on the occurrence of Campylobacter jejuni at four California chicken ranches. Journal of Food Protection 47, 293–298.CrossRefGoogle ScholarPubMed
Snoeyenbos, G. H., Weinack, O. M. & Smyser, C. F. (1978). Protecting chicks and poults from salmonella by oral administration of ‘normal’ gut microflora. Avian Diseases 22, 273–287.CrossRefGoogle ScholarPubMed
Soerjadi, A. S., Snoeyenbos, G. H. & Weinack, O. M. (1982). Intestinal colonisation and competitive exclusion of Campylobacter fetus subsp. jejuni in young chicks. Avian Diseases 26, 520–524.CrossRefGoogle ScholarPubMed
Soerjadi-Liem, A. S., Synoeyenbos, G. H. & Weinack, D. M. (1984). Comparative studies on competitive exclusion of three isolates of Campylobacter fetus subsp. jejuni in chickens by native gut microflora. Avian Diseases 28, 139–146.CrossRefGoogle ScholarPubMed
Welkos, S. L. (1984). Experimental gastroenteritis in newly-hatched chicks infected with Campylobacter jejuni. Journal of Medical Microbiology 18, 233–248.CrossRefGoogle ScholarPubMed
Wempe, J. M., Genigeorgis, C. A., Farver, T. B. & Yusufu, H. I. (1983). Prevalence of Campylobacter jejuni in two California chicken processing plants. Applied and Environmental Microbiology 45, 355–359.CrossRefGoogle ScholarPubMed