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Experimental phycomycosis in mice; examination of the role of acquired immunity in resistance to Absidia ramosa

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel  and
Susan M. Eades
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
Susan M. Eades
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
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Attempts were made to stimulate acquired immunity to experimental Absidia ramosa infection in mice. Unprotected animals inoculated with large doses of A. ramosa spores frequently developed acute phycomycosis of the central nervous system. Mice previously exposed to sub-lethal doses of spores showed a high resistance to subsequent challenge with A. ramosa. No consistent increase in resistance was observed in mice vaccinated with killed A. ramosa spores, hyphal walls, intracellular mycelial antigens or various combinations of these, with Freund's incomplete adjuvant.

Antibodies to soluble mycelial antigens were inconsistently present in the sera of mice vaccinated with sub-lethal doses of viable spores. They were generally present in the sera of animals vaccinated with mycelial extracts or hyphal walls but not killed spores.

Delayed hypersensitivity reactions to A. ramosa mycelial antigens could usually be elicited by intradermal tests in mice exposed to viable spores but irregularly in those vaccinated with non-viable preparations. Positive reactions were also frequently given by older mice not deliberately exposed to A. ramosa.

Although mice previously exposed to viable A. ramosa spores were highly resistant to intravenous or intracerebral challenge with this fungus, they were more likely to develop persistent local granulomata on subcutaneous injection of spores than were unvaccinated animals.

Type
Research Article
Information
Journal of Hygiene , Volume 77 , Issue 2 , October 1976 , pp. 221 - 233
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Ainsworth, G. C. & Austwick, P. K. C. (1959). In Fungal Diseases of Animals. Farnham Royal, Slough: Commonwealth Agricultural Bureaux.Google Scholar
Amos, W. M. G. (1970). The extraction of fungal antigens and their use in serological tests as an aid to the diagnosis of bronchial disorders. Journal of Medical Laboratory Technology 27, 16.Google Scholar
Baker, R. D. (1971). In Handbuch der speziellen pathologischen Anatomie and Histologie(ed. Uehlinger, E.), Vol. 5/part 3. The pathologic anatomy of mycoses. Berlin, Heidelberg and New York: Springer-Verlag.Google Scholar
Cohn, Z. A. (1963). The fate of bacteria within phagocytic cells. II. The modification of intracellular degradation. Journal of Experimental Medicine 117, 43.CrossRefGoogle ScholarPubMed
Corbel, M. J. & Eades, S. M. (1973). The effect of soluble extracts of bovine placenta on the growth of fungi implicated in bovine mycotic abortion. British Veterinary Journal 129, lxxv.Google Scholar
Corbel, M. J. & Eades, S. M. (1975). Factors determining the susceptibility of mice to experimental phycomycosis. Journal of Medical Microbiology 8, 551.Google Scholar
Corbel, M. J., Pepin, G. A. & Millar, P. G. (1973). The serological response to Aspergillus fumigatus in experimental mycotic abortion in sheep. Journal of Medical Microbiology 6, 539.CrossRefGoogle ScholarPubMed
Eades, S. M. & Corbel, M. J. (1975). Metastatic sub-cutaneous zygomycosis following intravenous and intracerebral inoculation of Absidia corymbifera spores. Sabouraudia 13, 200.CrossRefGoogle Scholar
Emmons, C. W., Binford, C. H. & Utz, J. P. (1970). In Medical Mycology, 2nd edn. Philadelphia: Lea and Febiger.Google Scholar
Kong, Y. C. M. & Levine, H. B. (1967). Experimentally induced immunity in the mycoses Bacteriological Reviews 31, 35.CrossRefGoogle ScholarPubMed
Mariano, M. & Spector, W. G. (1974). The formation and properties of macrophage polykaryons (inflammatory giant cells). Journal of Pathology 113, 1.Google Scholar
Medawar, P. B. (1948). Immunity to homologous grafted skin. III. Fate of skin homografts transplanted to brain, to subcutaneous tissue and to anterior chamber of eye. British Journal of Experimental Pathology 29, 58.Google Scholar
Ouchterlony, Ö. (1953). Antigen-antibody reactions in gels. Acta pathologica et microbiologica scandinavica 32, 231.Google Scholar
Smith, G. R. (1972). Experimental aspergillosis in mice: aspects of resistance. Journal of Hygiene 70, 741.Google Scholar
Smith, J. M. B. & Jones, R. H. (1973). Localisation and fate of Absidia ramosa spores after intravenous inoculation of mice. Journal of Comparative Pathology 83, 49.CrossRefGoogle ScholarPubMed
Spector, W. G., Reichhold, N. & Ryan, G. B. (1970). Degradation of granuloma-inducing micro-organisms by macrophages. Journal of Pathology 101, 339.Google Scholar