Hostname: page-component-745bb68f8f-f46jp Total loading time: 0 Render date: 2025-01-27T04:42:12.208Z Has data issue: false hasContentIssue false
  • English
  • Français

Factors affecting the growth of virus of foot-and-mouth disease on surviving explants of bovine tongue epithelium

Published online by Cambridge University Press:  15 May 2009

A. W Morrow  and
L. S Buckley
A. W Morrow
Affiliation:
Research Institute(Animal Virus Diseases), Pirbright, Surrey
L. S Buckley
Affiliation:
Research Institute(Animal Virus Diseases), Pirbright, Surrey
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A simple, laboratory scale, stirred system has been developed to examine conditions of culture necessary for optimum return of infective virus from explants of surviving bovine tongue epithelium, with particular bias towards large-scale procedures.

Control of hydrogen-ion concentration and aeration of the culture are emphasized.

The authors wish to thank Dr I. A. Galloway and Dr J. B. Brooksby for their helpful advice in preparing this paper, Mrs J. Lansley for her untiring technical assistance and Mr J. Goodridge who constructed the culture apparatus.

Type
Research Article
Information
Journal of Hygiene , Volume 62 , Issue 1 , March 1964 , pp. 101 - 113
Copyright
Copyright © Cambridge University Press 1964

References

REFERENCES

Baron, S., Porterfield, J. S. & Isaacs, A. (1961). Influence of oxygenation on virus growth. Virology, 14, 444.CrossRefGoogle ScholarPubMed
Brooksby, J. B. & Wardle, E. (1954). Titration of the virus of foot-and-mouth disease in culture. J. Hyg., Camb., 52, 87.CrossRefGoogle ScholarPubMed
Cooper, C. M., Fernstrom, G. A. & Miller, S. A. (1944). Performances of agitated gasliquid contactors. Industr. Engng. Chem. (industr.), 36, 504.CrossRefGoogle Scholar
Dickens, F. (1959). Intracellular regulation of carbohydrate metabolism. Aust. J. exp. Biol. med. Sci. (Spec. Suppl.), 37, 1.Google Scholar
Finn, R. K. (1954). Agitation-aeration in the laboratory and in industry. Bact. Rev. 18, 254.CrossRefGoogle ScholarPubMed
Frenkel, H. S. (1947). La culture du virus do la flèvre aphteuse sur l'épithélium de Ia langue des bovidés. Bull. Off. int. Epiz. 28, 155.Google Scholar
Frenkel, H. S. (1951). Research on foot-and-mouth disease. III. The cultivation of the virus on a practical scale in oxplantations of bovine tongue epithelium. Amer. J. vet. Res. 12, 187.Google ScholarPubMed
Frenkel, H. S. (1953). Recherches sur la fièvre aphteuse. La culture du virus sur une échelle pratique par explantation d'épithélium lingual bovin. Bull. Off. Int. Epiz. 39, 91.Google Scholar
Frenkel, H. S. (1954). Research on foot-and-mouth disease. VII. Cultivation of the virus on a practical scale in explantations of tongue epithelium. A modification of the culture technique. Amer. J. vet. Res. 15, 346.Google ScholarPubMed
Frenkel, H. S. & Ribelin, W. E. (1956). Cultivation of the foot-and-mouth disease virus in explanted epithelium of the bovine tongue. IX. Growth of the virus in large scale tissue culture. Amer. J. vet. Res. 17, 40.Google Scholar
Galloway, I. A. (1962). Results of the use of two live attenuated strain vaccines in controlling outbreaks of foot-and-mouth disease. Bull. Off. Int. Epiz. 57, 748.Google Scholar
Harding, V. J. & Downs, C. E. (1933). Notes on the Schaffer-Somogyi copper reagent. J. biol. Chem. 101, 487.CrossRefGoogle Scholar
Harding, V. J., Nicholson, T. F., Grant, G. A., Hern, G. & Dowins, C. E. (1932). Preliminary account of analytical methods for individual carbohydrates. Trans. roy. Soc. Can., 3rd Series, 26, Sect. V.Google Scholar
Henderson, W. M. (1953). The use of virus culture in foot-and-mouth disease research. Proc. XVth Int. vet. Congr. Part 1, 191.Google Scholar
Hixon, A. W. & Gaden, E. L. (1950). Oxygen transfer in submerged fermentation. Industr. Engng. Chem. (industr.), 42, 1792.CrossRefGoogle Scholar
Minami, S. (1923). Versuche an überlebendem Carcinomgewebe. Biochem. Z. 142, 334.Google Scholar
Patty, R. E., Bachrach, H. L. & Hess, W. R. (1960). Growth of foot-and-mouth disease virus in bovine kidney cell suspension. Amer. J. vet. Res. 21, 144.Google Scholar
Sellers, R. F. (1959). The growth and antigenicity of the virus of foot-and-mouth disease in pig kidney tissue cultures. Arch. ges. Virusforsch. 9, 621.CrossRefGoogle Scholar
Skinner, H. H. (1951). Propagation of strains of foot-and-mouth disease virus in unweaned white mice. Proc. R. Soc. Med. 44., 1041.Google ScholarPubMed
Subak-Sharpe, H. (1961). The quantitative study of foot-and-mouth disease virus in unweaned mice. Arch. ges. Virusforsch. 11, 1.CrossRefGoogle Scholar
Taylor-Robinson, D., Zwartow, H. T. & Westwood, J. C. N. (1961). Oxygen requirement for poliovirus replication in concentrated suspensions of ERK cells. Brit. J. exp. Path. 42, 317.Google ScholarPubMed