Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-27T12:53:36.646Z Has data issue: false hasContentIssue false
  • English
  • Français

Flagellin gene polymorphism analysis of Campylobacter jejuni infecting man and other hosts and comparison with biotyping and somatic antigen serotyping

Published online by Cambridge University Press:  15 May 2009

R. J. Owen ,
C. Fitzgerald ,
K. Sutherland  and
P. Borman
R. J. Owen
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Avenue., London NW9 5HT
C. Fitzgerald
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Avenue., London NW9 5HT
K. Sutherland
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Avenue., London NW9 5HT
P. Borman
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Avenue., London NW9 5HT
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Flagellin gene sequence polymorphisms were used to discriminate amongst 77 strains of Campylobacter jejuni from sporadic and outbreak-associated human enteric infections, and from chickens, sheep and calves. The results were assessed in relation to Lior biotyping and serotyping (Penner somatic antigens). Eight DNA PCR-RFLP patterns (genotypes) were identified by analysis of HinfI fragment length polymorphisms in flagellin gene (flaA) polymerase chain reaction (PCR) products. One genotype (F-l) was a feature of 55% of strains. Strains within the genotypes were heterogeneous with respect to somatic antigens with 12 serogroups represented amongst the C. jejuni isolates of flaA type F-l. Serogroups Pen 1. 2 and 23 were the commonest (45%) amongst the 20 different serogroups represented. Several unique clusters of isolates with diverse biotypes were defined, and one cluster (F-7/Pen 23) contained epidemiologically implicated outbreak strains as well as sheep and calf isolates. We conclude that HinfI flaA typing is reproducible and offers high typability, and its combination with serogrouping provides a novel approach to characterizing isolates of C. jejuni with improved discrimination.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 2 , October 1994 , pp. 221 - 234
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Healing, TD, Greenwood, MH, Pearson, ADCampylobacter and enteritis. Rev Med Microbiol 1992; 3: 159–67.Google Scholar
2.PHLS Communicable Disease Surveillance Centre. Other gastrointestinal tract infections. England and Wales. Regular summary data. Comm Dis Rep 1993; 3: 11.Google Scholar
3.Communicable Disease Review. Campylobacteriosis. J Public Health Med 1993; 15: 202–8.Google Scholar
4.Patton, CM, Wachsmuth, IK, Evins, GM et al. . Evaluation of 10 methods to distinguish epidemic-associated Campylobacter strains. J Clin Microbiol 1991; 29: 680–8.CrossRefGoogle ScholarPubMed
5.Patton, CM, Wachsmuth, IK Typing schemes: are current methods useful ? In: Nachamkin, I, Blaser, MJ, Tompkins, LS eds. Campylobacter jejuni. Current status and future trends. Washington: ASM 1992: 110–23.Google Scholar
6.Hernandez, J, Owen, RJ, Costas, M, Lastovica, ADNA-DNA hybridization and analysis of restriction endonuclcase and rRNA gene patterns of atypical (catalase- weak/negative) Campylobacter jejuni from paediatrie blood and faecal cultures. J Appl Bacteriol 1991; 70: 71–80.CrossRefGoogle Scholar
7.Fayos, A, Owen, RJ, Desai, M, Hernandez, JRibosomal RNA gene restriction fragment diversity amongst Lior biotypes and Penner serotypes of Campylobacter jejuni and Campylobacter coli. FEMS Microbiol Lett 1992; 95: 87–94.CrossRefGoogle Scholar
8.Hernandez, J, Fayos, A, Owen, RJBiotypes and DNA ribopatterns of thermophilic eampylobacters from faeces and seawater in Eastern Spain. Lett Appl Microbiol 1991; 13: 207–11.CrossRefGoogle Scholar
9.Owen, RJ, Hernandez, J, Bolton, FDNA restriction digest and ribosomal RNA gene patterns of Campylobacter jejuni: a comparison with bio-, sero- and bacteriophage-types of United Kingdom outbreak strains. Epidemiol Infect 1990; 105: 265–75.CrossRefGoogle ScholarPubMed
10.Owen, RJ, Hernandez, J Ribotyping and arbitrary-primer PCR fingerprinting of campylobacters. In: Kroll, RG, Gilmour, A, Sussman, M eds. New techniques in food and beverage microbiology. SAB Technical Series 31. London: Academic Press. 1993; 265–85.Google Scholar
11.Taylor, DEGenetics of Campylobacter and Helicobacter. Ann Rev Microbiol 1992; 46: 35–64.CrossRefGoogle ScholarPubMed
12.Khawaja, R, Neote, K, Bingham, HL et al. . Cloning and sequence analysis of the flagellin gene of Campylobacter jejuni TGH 9011. Curr Microbiol 1992; 24: 217–21.CrossRefGoogle Scholar
13.Nuijten, PMM, Van Asten, FJAM, Gaastra, W, van der Zeijst, BAMStructural and functional analysis of two Campylobacter jejuni flagellin genes. J Biol Chem 1990; 265: 17798–804.CrossRefGoogle ScholarPubMed
14.Fischer, SH, Nachamkin, ICommon and variable domains of the flagellin gene. flaA. in Campylobacter jejuni. Molec Microbiol 1991; 5: 1151–8.CrossRefGoogle ScholarPubMed
15.Nuijten, PGM, Wassenaar, TM, Newell, DG, van der Zeijst, BAM Molecular characterization and analysis of Campylobacter jejuni flagellin genes and proteins. In: Nachamkin, I, Blaser, MJ, Tompkins, LS eds. Campylobacter jejuni. Current status and future trends. Washington: ASM. 1992: 282–95.Google Scholar
16.Nachamkin, I, Bohachick, K, Patton, CMFlagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol 1993; 31: 1531–6.CrossRefGoogle ScholarPubMed
17.Owen, RJ, Fayos, A, Hernandez, JPCR-based RFLP analysis of DNA sequence diversity of flagellin genes of Campylobacter jejuni and allied species. Mol Cell Probes 1993; 7: 471–80.CrossRefGoogle ScholarPubMed
18.Birkenhead, D, Hawkey, PM, Heritage, J, Gascoyne-Binzi, DM, Kite, PPCR for the detection and typing of campylobacters. Lett Appl Microbiol 1993; 17: 235–7.CrossRefGoogle ScholarPubMed
19.Penner, JL, Hennessey, JNPassive haemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat stable antigens. J Clin Microbiol 1980; 12: 732–7.CrossRefGoogle ScholarPubMed
20.Barrow, GI, Feltham, RRA eds. Cowan and Steel's Manual for the identification of medical bacteria, 3rd edn.Cambridge: Cambridge University Press. 1993.CrossRefGoogle Scholar
21.Benjamin, J, Leaper, S, Owen, RJ, Skirrow, MBDescription of Campylobacter laridis. a new species comprising the nalidixic acid resistant thermophilic Campylobacter (NARTC) group. Curr Microbiol 1983; 8: 231–8.CrossRefGoogle Scholar
22.Lior, HNew extended biotyping scheme for Campylobacter jejuni. Campylobacter coli. and Campylobacter laridis. J Clin Microbiol 1984; 20: 636–40.CrossRefGoogle ScholarPubMed
23.King, V, Clayton, CLGenomic investigation of phenotypic variation in Campylobacter jejuni flagellin. FEMS Microbiol Lett 1991; 84: 107–12.CrossRefGoogle Scholar
24.Wilson, K Preparation of genomic DNA from bacteria. In: Ausubel, FM, Brent, R, Kingston, RE et al. , eds. Current protocols in molecular biology. New York: Wiley, 1987; 2.4.1–2.4.2.Google Scholar
25.Sneath, PHA, Sokal, RRNumerical taxonomy. San Francisco: W. H. Freeman Co., 1973.Google Scholar
26.Costas, MNumerical analysis of sodium dodecylsulphate-polyacrylamide gel electrophoretic protein patterns for the classification, identification and typing of medically important bacteria. Electrophoresis 1990; 11: 382–91.CrossRefGoogle Scholar
27.Wassenaar, TM, Newell, DG, van der Zeijst, BAMIntermoleeular and intramolecular recombinations between flagellin genes of Campylobacter jejuni. Acta Gastro-Enterologica Belgica 1993; 56 (suppl): 16.Google Scholar
28.Preston, MA, Penner, JLCharacterisation of cross-reacting serotypes of Campylobacter jejuni. Can J Microbiol 1989; 35: 265–73.CrossRefGoogle ScholarPubMed
29.Fayos, A, Owen, RJ, Hernandez, J, Jones, C, Lastovica, AMolecular sub-typing by genome and plasmid analysis of Campylobacter jejuni serogroups 01 and 02 (Penner) from sporadic and outbreak cases of human diarrhoea. Epidemiol Infect 1993; 111: 415–27.CrossRefGoogle Scholar