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Genomic fingerprints of Staphylococcus aureus of bovine origin by polymerase chain reaction-based DNA fingerprinting

Published online by Cambridge University Press:  15 May 2009

K. R. Matthews ,
S. J. Kumar ,
S. A. O'Conner ,
R. J. Harmon ,
J. W. Pankey ,
L. K. Fox  and
S. P. Oliver
K. R. Matthews*
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Knoxville, Tennessee, USA
S. J. Kumar
Affiliation:
Department of Animal Science, Institute of Agriculture, The University of Tennessee, Knoxville, Tennessee 37901–1071, USA
S. A. O'Conner
Affiliation:
Department of Animal Science, Institute of Agriculture, The University of Tennessee, Knoxville, Tennessee 37901–1071, USA
R. J. Harmon
Affiliation:
Department of Animal Science, University of Kentucky, Lexington, Kentucky 40546–0215, USA
J. W. Pankey
Affiliation:
Department of Animal Sciences, University of Vermont, South Burlington, Vermont 05403, USA
L. K. Fox
Affiliation:
Department of Veterinary Clinical Medicine and Surgery, Washington State University, Pullman, Washington 99164, USA
S. P. Oliver
Affiliation:
Department of Animal Science, Institute of Agriculture, The University of Tennessee, Knoxville, Tennessee 37901–1071, USA
*
*Author for correspondence to: K. R. Matthews, Department of Animal Science, 109 McCord Hall, 2460 Morgan Circle, Knoxville, TN 37901–1071, USA
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Summary

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Staphylococcus aureus (n = 75) isolated from mammary secretions of cows with subclinical and clinical mastitis from several geographic locations in the USA were examined using polymerase chain reaction-based DNA fingerprinting. DNA fingerprints were produced using a synthetic oligonucleotide primer (5‘GTAACGCC3’) to produce a distinct spectrum of amplified DNA fragments facilitating a high degree of resolution for differentiating S. aureus strains. PCR-based DNA fingerprinting grouped the 75 S. aureus isolates into 19 distinct profiles. The technique differentiated closely related strains within and between geographic locations. Findings suggest that certain types are found across geographic regions suggesting a common clonal type. Within herd data suggest heterogeneity among subclinical and clinical isolates of S. aureus strains. Compared to existing typing methods, PCR-based DNA fingerprinting is easy to perform and interpret. Use of PCR-based DNA fingerprinting may allow for a more detailed investigation of the epidemiology of S. aureus mastitis in dairy cows.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 1 , February 1994 , pp. 177 - 186
Copyright
Copyright © Cambridge University Press 1994

References

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