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Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal

Published online by Cambridge University Press:  15 May 2009

F. Bacellar ,
R. L. Regnery ,
M. S. Núncio  and
A. R. Filipe
F. Bacellar
Affiliation:
Centra de Estudos de Vectores e Doenças Infecciosas do Institute National de Saúde, 2965 Áquas de Moura, Portugal
R. L. Regnery
Affiliation:
Centers for Disease Control and Prevention, Division of Viral and Rickettsial Diseases, US Department of Health and Human Services, Public Health Service, Atlanta, Georgia 3033, USA
M. S. Núncio
Affiliation:
Centra de Estudos de Vectores e Doenças Infecciosas do Institute National de Saúde, 2965 Áquas de Moura, Portugal
A. R. Filipe*
Affiliation:
Centra de Estudos de Vectores e Doenças Infecciosas do Institute National de Saúde, 2965 Áquas de Moura, Portugal Escola National de Saúde Pública, Universidade Nova de Lisboa, 1699 Lisboa, Portugal
*
*Professor Armindo R. Filipe, Centre de Estudos de Vectores e Doenças Infecciosas, 2965 Aguas de Moura, Portugal.
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Summary

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Twelve rickettsial isolates, from Rhipicephalus sanguineus, R. turanicus, Dermacentor marginatus and Hyalomma marginatus, were subjected to genotypic analysis. Amplification of specific DNA sequences, restriction endonuclease digestion of amplified DNA products, and gel electrophoresis were used to identify specific DNA fragment-banding patterns. Five patterns were resolved. Four were homologous with those of previously described rickettsial genotypes, R. conorii, R. slovaca, R. rhipicephali and R. massiliae. The fifth pattern differed by only a single altered restriction endonuclease cleavage site. For the first time in Portugal a widely distributed spectrum of spotted fever group rickettsia was found among potential vector species stressing the need to determine their potential for human and domestic animals infection.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 169 - 178
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Péter, O, Raoult, D, Burgdorfer, W. Rickettsia helvetica, a new spotted fever group rickettsia: immunochemical analysis of the antigens of 5 spotted fever group rickettsiae. In Kazar, J, ed. Rickettsiae and rickettsial diseases. Bratislava, Czechoslovakia: Publishing House of the Slovak Academy of Sciences, 1985: 99–105.Google Scholar
2.Rehácek, J. Rickettsia slovaca, the organism and its ecology. Acta Slovaka Natur Brno, Czechoslovakia 1984; 18: 1–50.Google Scholar
3.Rehácek, J, Tasarevic, IV. Acari-borne Rickettsiae and Rickettsioses in Eurasia. Bratislava, Czechoslovakia: Publishing House of the Slovak Academy of Sciences, 1988.Google Scholar
4.Goldwasser, RA, Steiman, Y, Klingberg, W, Swartz, TA, Klingberg, MA. The isolation of strains of Rickettsiae of the spotted fever group in Israel and their differentiation from other members of the group by immunofluorescence methods. Scand J Infect Dis 1974; 6: 53–62.CrossRefGoogle Scholar
5.Manor, E, Ighbarieh, J, Sarov, B, Kassis, I, Regnery, R. Human and tick spotted fever group rickettsia isolates from Israel: a genotypic analysis. J Clin Microbiol 1992; 30: 2653–6.CrossRefGoogle Scholar
6. Direcção Geral Cuidados Saúde Primáries (DGCSP) (Serviço de Informação de Saúde). 1991. Doenças de declaração obrigatória 1985–1991, Lisboa, Portugal.Google Scholar
7.Filipe, AR, Bacellar, FC, David de Morais, JA. Anticorpos contra Rickettsiae na população do sul de Portugal. Rev Port Doen Infec 1990; 13: 85–9.Google Scholar
8.Tavares, L, Botas, J, Antunes, F, Araújo, FC. A febre escaro-nodular em Portugal. O Médico 1985; 113: 838–43.Google Scholar
9.Nobre, G. A cultura de Rickettsia conorii e a sua utilização em provas de imunofluorescência. Arq Inst Bact Câmara Pestana 1971; 11: 101–216.Google Scholar
10.Bacellar, F, Núncio, MS, Rehaeek, J, Filipe, AR. Rickettsiae and rickettsioses in Portugal. Eur J Epidemiol 1991; 7: 291–3.CrossRefGoogle ScholarPubMed
11.Filipe, AR, Rehacek, J, Bacellar, FC.Núncio, MS. Microbiologia e parasitologia da hemolinfa dos Ixodídeos do Distrito de Setúbal. Rev Port Ciên Vet 1992; 87: 46–52.Google Scholar
12.Júlio, S, Núncio, MS, Bacellar, F, Filipe, AR. Contribuiçáo para o estudo da dinâmica das populaões de Ixodídeos de uma zona rural ao sul do Tejo. 1° Simpósio Ibérico sobre Ixodoidea e Doenças Transmissíveis. Zaragoza, Spain, 12 1991.Google Scholar
13.Péter, O, Raoult, D, Gilot, B. Isolation by a sensitive centrifugation cell culture system of 52 strains of spotted fever group Rickettsiae from ticks collected in France. J Clin Microbiol 1990; 28: 1597–9.CrossRefGoogle ScholarPubMed
14.Regnery, RL, Spruill, CL, Plikaytis, BD. Genotypic identification of Rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol 1991; 173: 1576–89.CrossRefGoogle ScholarPubMed
15.Burgdorfer, W. Hemolymph test. Am J Trop Med Hyg 1970; 19: 1010–4.CrossRefGoogle ScholarPubMed
16.Giménez, DF. 1964. Staining Rickettsiae in yolk-sac cultures. Stain Tech 1964; 39: 135–40.CrossRefGoogle ScholarPubMed
17.Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning: a laboratory manual, 2nd ed.Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 1989.Google Scholar
18.Anderson, Be, McDonald, GA.Jones, DC, Regnery, RL. A protective antigen of Rickettsia rickettsii has tandemly repeated, near-identical sequences. Infect Immun 1990; 58: 2760–9.CrossRefGoogle ScholarPubMed
19.Beati, L, Raoult, D. Rickettsia massiliae sp. nov., a new spotted fever group rickettsia. Intern J Sys Bacteriol 1993; 43: 839–40.CrossRefGoogle Scholar
20.Drancourt, M, Regnery, R, Raoult, D. Identification of tick-isolates by centrifugation-shell vial assay followed by polymerase chain reaction and restriction-endonuclease length polymorphism analysis. In Kazar, J, Raoult, D, eds. Rickettsiae of rickettsial disease Bratislava, Czechoslovakia: Publishing House of the Slovak Academy of Sciences, 1991: 232–7.Google Scholar
21.Beati, L, Finidori, J-P, Raoult, D. First isolation of Rickettsia slovaca from Dermacentar marginatus in France. Am J Trop Med Hyg 1993; 48: 257–68.CrossRefGoogle Scholar
22.Caeiro, VMP. As carraças em Portugal: Seus hospedeiros domésticos e silvestres. Ciclos vitais, preferências de vegetação e de clima. O Médico Veterinário 1992; 28: 7–25.Google Scholar
23.Burgdorfer, W, Sexton, DJ, Gerloff, RK, Anacker, RL, Philip, RN, Thomas, LA. Rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the Unites States. Infect Immun 1975; 12: 205–10.CrossRefGoogle Scholar
24.Beati, L, Finidori, J-P, Gilot, B, Raoult, D. Comparison of serologic typing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein analysis, and genetic restriction fragment length polymorphism analysis for identification of rickettsiae: characterization of two new rickettsial strains. J Clin Microbiol 1992; 30: 1922–30.CrossRefGoogle ScholarPubMed
25.Dessouky, E, Manor, EA, Lange, JV, Azad, AF. Detection of spotted fever group Rickettsiae in ticks from the North Sinai Egypt. In Proc. IVth Intern. Symp. Rickettsia and Rickettsial Diseases. Bratislava, Czechoslovakia: Publishing House of the Slovak Academy of Sciences, 1991: 383–8.Google Scholar
26.Kelly, PJ, Mason, PR. Serological typing of spotted fever group rickettsia isolates from Zimbabwe. J Clin Microbiol 1990; 28: 2302–4.CrossRefGoogle ScholarPubMed