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Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains

Published online by Cambridge University Press:  25 March 2010

P. F. Nettleton ,
Marilyn J. Davies  and
M. M. Rweyemamu
P. F. Nettleton
Affiliation:
Wellcome Foot-and-Mouth Disease Vaccine Laboratory, Wellcome Biotechnology Limited, Ash Road, Pirbright, Surrey
Marilyn J. Davies
Affiliation:
Wellcome Foot-and-Mouth Disease Vaccine Laboratory, Wellcome Biotechnology Limited, Ash Road, Pirbright, Surrey
M. M. Rweyemamu
Affiliation:
Wellcome Foot-and-Mouth Disease Vaccine Laboratory, Wellcome Biotechnology Limited, Ash Road, Pirbright, Surrey
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Summary

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A study of the ability of 49 strains of FMD virus to replicate in BHK-21 monolayer cells maintained under a standard agar overlay containing 5·2 mM guanidine hydrochloride and to withstand heat inactivation at 54°C for 1 h showed that strains belonging to serotypes C, O and Asia 1 were generally more resistant to guanidine and heat stable than the SAT 1, 2 and 3 serotypes. The type A viruses as a whole occupied an intermediate position between these two groups.

In vitro passage in BHK-21 cells influenced the guanidine sensitivity of 3(O, C and SAT 3) of the 7 FMD serotypes suggesting that this is not a stable genetic marker. Heat stability of the FMD viruses, however, did not change on passage, suggesting that this is a stable characteristic inherent in any homogeneous FMD virus population.

Type
Research Article
Information
Journal of Hygiene , Volume 89 , Issue 1 , August 1982 , pp. 129 - 138
Copyright
Copyright © Cambridge University Press 1982

References

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