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Incubation at 44°C. as a test for faecal coli

Published online by Cambridge University Press:  15 May 2009

L. F. L. Clegg ,
H. P. Sherwood  and
R. W. Dodgson
L. F. L. Clegg
Affiliation:
Fisheries Experiment Station, Conway, Caernarvonshire
H. P. Sherwood
Affiliation:
Fisheries Experiment Station, Conway, Caernarvonshire
R. W. Dodgson
Affiliation:
Consultant for Shellfish Services (late Director), Ministry of Agriculture and Fisheries
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1. The use of Esch. coli alone as an index of faecal pollution for shellfish, and the correlation between the 44° C. MacConkey test and citrate tests are discussed.

2. The mercury-toluene thermo-regulator used in these experiments, which gives a maximum variation of ± 0.1° C., is discussed briefly and illustrated.

3. Experiments are described in which 522 colonies from polluted shellfish were isolated, inoculated into MacConkey's broth and incubated at temperatures of 37° C. and at successive 1° intervals from 41 to 46° C., in accurately controlled water-baths. An almost perfect negative correlation was found to exist between 44° C. incubation and the citrate test.

4. It appeared that temperatures above 44° C. are detrimental to the growth of Esch. coli.

5. Certain cultures of citrate-negative lactose fermenters at 37° C., which were inhibited at 44° C., were found on further investigation to be mostly intermediate types.

Type
Research Article
Information
Journal of Hygiene , Volume 39 , Issue 4 , July 1939 , pp. 361 - 374
Copyright
Copyright © Cambridge University Press 1939

References

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