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Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis

Published online by Cambridge University Press:  26 June 2006

P. FARNIA ,
M. R. MASJEDI ,
B. NASIRI ,
M. MIRSAEDI ,
S. SOROOCH ,
M. KAZEAMPOUR  and
A. A. VELAYATI
P. FARNIA
Affiliation:
National Research Institute of Tuberculosis and Lung Disease (NRITLD), Darabad, Tehran, Iran
M. R. MASJEDI
Affiliation:
National Research Institute of Tuberculosis and Lung Disease (NRITLD), Darabad, Tehran, Iran
B. NASIRI
Affiliation:
National Research Institute of Tuberculosis and Lung Disease (NRITLD), Darabad, Tehran, Iran
M. MIRSAEDI
Affiliation:
National Research Institute of Tuberculosis and Lung Disease (NRITLD), Darabad, Tehran, Iran
S. SOROOCH
Affiliation:
National Research Institute of Tuberculosis and Lung Disease (NRITLD), Darabad, Tehran, Iran
M. KAZEAMPOUR
Affiliation:
National Research Institute of Tuberculosis and Lung Disease (NRITLD), Darabad, Tehran, Iran
A. A. VELAYATI
Affiliation:
National Research Institute of Tuberculosis and Lung Disease (NRITLD), Darabad, Tehran, Iran
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Abstract

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The stability of IS6110 restriction fragment length polymorphism (RFLP) pattern was determined in 31 isolates from patients with multidrug-resistant tuberculosis (MDR-TB). These patients were in actual chains of transmission and they referred to the National Institute of Tuberculosis and Lung Diseases, Tehran, Iran. Susceptibility testing against first- and second-line drugs were performed by the proportional method on Lowenstein–Jensen culture media. Thereafter, DNA fingerprinting by IS6110 with direct repeat (DR) region as a probe was performed by standard protocols. The rate of IS6110 changes was 16%, although, no variation was found in the DR region, in a time-span of 1–63 months. The strains with unstable IS6110 patterns were resistant to all drugs tested, and the majority of them (60%) were collected from HIV-positive patients. The results demonstrated that for a reliable interpretation of strain typing, it is better to use an additional marker along with IS6110 RFLP.

Type
Research Article
Information
Epidemiology & Infection , Volume 135 , Issue 2 , February 2007 , pp. 346 - 352
Copyright
2006 Cambridge University Press