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Isolation and properties of an RNA fraction present in Brucella culture supernatants

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel  and
R. A. Brewer
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
R. A. Brewer
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
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The supernatant fluids of batch and continuous cultures of Brucella strains contained up to 100 mg/i of soluble RNA which could be recovered by precipitation with lysozyme. This RNA fraction had many of the properties of ribosomal RNA and was single-stranded, sensitive to ribonuclease, with an approximate sedimentation constant of 5S, a molecular weight of about 35000 daltons and an adenine; guanine; cytosine; uracil content of 17·5 26·5 33; 23 mol% respectively. RNA fractions from lysozyme precipitates evoked high titres of Brucella agglutinins on injection into rabbits and induced acute inflammatory responses in guinea-pig skin. Highly purified RNA fractions prepared by phenol extraction of lysozyme precipitates did not evoke antibodies to Brucella abortus.

Type
Research Article
Information
Journal of Hygiene , Volume 84 , Issue 2 , April 1980 , pp. 223 - 236
Copyright
Copyright © Cambridge University Press 1980

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