Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-14T22:12:33.106Z Has data issue: false hasContentIssue false
  • English
  • Français

Legionella pneumophila serogroup 1 population in Italy by monoclonal subtyping

Published online by Cambridge University Press:  15 May 2009

M. Castellani Pastoris ,
M. McIntyre  and
P. Goldoni
M. Castellani Pastoris*
Affiliation:
Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy
M. McIntyre
Affiliation:
Department of Microbiology, John Radcliffe Hospital, Oxford 0X3 9DU, UK
P. Goldoni
Affiliation:
Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy
*
Correspondence and requests for reprints to: M. Castellani Pastoris, Istituto Superiore di Sanita. Department of Bacteriology and Medical Mycology, Viale Regina Elena 299, 00161 Rome, Italy.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The Oxford panel of monoclonal antibodies was used to subtype 83 strains of Legionella pneumophila serogroup 1 of human and environmental origin. The International panel was also used to subtype 50 of them. All the 18 patients' isolates were of the Pontiac subgroup, and 40/43 of the environmental strains of the Pontiac subgroup were associated with human infection. The remaining environmental strains were subgroups Olda (15 strains), Camperdown (5 strains), and Bellingham (2 strains). The Philadelphia subgroup was the commonest among the environmental strains tested with the international MABs panel.

This study confirms previous findings that L. pneumophila serogroup 1 isolates with the Pontiac (Oxford panel) or MAB-2 (international panel) reacting antigen marker seem to be more virulent than the other subgroups.

Type
Special Article
Information
Epidemiology & Infection , Volume 105 , Issue 1 , August 1990 , pp. 169 - 174
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Brown, A, Vickers, RM, Edler, EM, Lema, M, Garrity, GM. Plasmid and surface antigen markers of endemic and epidemic Legionella pneumophila strains. J. Clin Microbiol 1982; 16; 230–5.CrossRefGoogle ScholarPubMed
2.Collins, MT, Cho, SN, Hoiby, X, Espersen, F, Back, L, Rief, JS. Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens. Infect Immun 1983; 39: 1428–40.CrossRefGoogle ScholarPubMed
3.Joly, JR, Kenny, GE. Antigenic analysis of Legionella pneumophila and Tatlockia micdadei (Legionella micdadei) by two dimensional (crossed) immunoelectrophoresis. Infect Immun 1982:35:721–9.CrossRefGoogle ScholarPubMed
4.Thomason, BM, Bibb, WF. Use of absorbed antisera for demonstration of antigenic variation among strains of Legionella pneumophila serogroup 1. J Clin Microbiol 1984; 19: 794–9.CrossRefGoogle ScholarPubMed
5.Guillet, JG, Hoebeke, J, Tram, C, Marullo, S, Strosberg, D. Characterization serological specificity and diagnostic possibilities of monoclonal antibodies against Legionella pneumophila. J Clin Microbiol 1983; 18: 793–7.CrossRefGoogle ScholarPubMed
6.Joly, JR, Chen, YY, Ramsay, D. Serogrouping and subtyping of Legionella pneumophila with monoclonal antibodies. J Clin Microbiol 1983; 18: 1040–6.CrossRefGoogle ScholarPubMed
7.McKinney, RM, Thacker, L, Wells, DE, Wong, MC, Jones, WJ, Bibb, WF. Monoclonal antibodies to Leyionella pneumophila serogroup 1: Possible applications in diagnostic tests and epidemiologic studies. Zentralbl Bakteriol Abt 1: Orig [Reihe] A 1983; 255: 91–5.Google ScholarPubMed
8.Miyazaki, T, Koga, H, Nakashima, M et al. , Production of monoclonal antibodies against Legionella pneumophila serogroup 1. Microbiol Immunol 1985; 29: 275–84.CrossRefGoogle ScholarPubMed
9.Para, MF, Plouffe, JF. Production of monoclonal antibodies to Legionella pneumophila serogroups 1 and 6. Clin. Microbiol 1983; 18: 895–900.CrossRefGoogle ScholarPubMed
10.Sethi, KK, Driieke, V, Brandis, H. Hybridoma-derived monoclonal immunoglobulin M antibodies to Legionella pneumophila serogroup 1 with diagnostic potential. J Clin Microbiol 1983: 17: 953–7.CrossRefGoogle ScholarPubMed
11.Watkins, ID, Tobin, JO'H. Studies with monoclonal antibodies to Legionella species. In: Thornsberry, C, Balows, A, Feeley, JC, Jakubowski, W, eds. Legionella: Proceedings of the 2nd International Symposium. Washington DC: American Society for Microbiology 1984: 259–62.Google Scholar
12.Joly, JK, McKinney, RM, Tobin, JO'H.Bibb, WF, Watkins, ID, Ramsay, D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol 1986; 23: 768–71.CrossRefGoogle ScholarPubMed
13.Garbe, PL, Davis, BJ, Weisfeld, JS et al. , Nosocomial Legionnaires' disease: Epidemiologic demonstration of cooling towers as a source. JAMA 1985; 254: 521–4.CrossRefGoogle ScholarPubMed
14.Joly, JR, Winn, WC. Correlation of subtypes of Legionella pneumophila defined by monoclonal antibodies with epidemiological classification of cases and environmental sources. J Infect Dis 1984; 150: 667–71.CrossRefGoogle ScholarPubMed
15.Meenhorst, PL, Reingold, AL, Groothuis, DG et al. , Water-related nosocomial pneumonia caused by Legionella pneumophila serogroup 1 and 10. J Infect Dis 1985; 152: 356–63.CrossRefGoogle ScholarPubMed
16.Moiraghi, Ruggenini A, Castellani, Pastoris M, Dennis, PJ et al. Legionella pneumophila in a hospital in Torino. Italy-A retrospective one-year study. Epidemiol Infect 1989; 102: 21–9.Google Scholar
17.Plouffe, if, Para, MF, Maher, WE, Hackman, B, Webster, L. Subtypes of Legionella pneumophila serogroup 1 associated with different attack rates. Lancet 1983; ii. 649–50.CrossRefGoogle Scholar
18.Ribeiro, CD, Burge, SH, Palmer, SR, Tobin, JO'H, Watkins, ID. Legionella pneumophila in a hospital water system following a nosocomial outbreak: prevalence, monoclonal antibody subgrouping and effect of control measures. Epidemiol Infect 1987; 98: 253–62.CrossRefGoogle Scholar
19.Ruf, B, Schurtnann, D, Horbach, I, Seidel, K, Pohle, HD. Nosocomial legionella pneumonia: demonstration of potable water as the source of infection. Epidemiol Infect 1988; 101: 647–54.CrossRefGoogle ScholarPubMed
20.Watkins, ID, Tobin, JO'H, Dennis, PJ, Brown, W, Newnham, RS, Kurtz, JB. Legionella pneumophila serogroup 1 subgrouping by monoclonal antibodies-an epidemiological tool. J Hyg 1985; 95 211–6.CrossRefGoogle ScholarPubMed
21.Brindle, RJ, Stannett, PJ, Tobin, JO'H. Legionella pneumophila: monoclonal antibody typing of clinical and environmental isolates. Epidemiol Infect; 99: 235–9.CrossRefGoogle Scholar
22.Dournon, E, Bibb, WF, Rajagopalan, P, Desplaces, N, McKinney, RM. Monoclonal antibody reactivity as a virulence marker for Legionella pneumophila serogroup 1 strains. J Infect Dis 1988; 157: 496–501.CrossRefGoogle ScholarPubMed